LOCUS       sequence-68921-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Ailion M, Jorgensen EM
  TITLE     Improved Mos1-mediated transgenesis in C. elegans.
  JOURNAL   Nat Methods. 2012 Jan 30;9(2):117-8. doi: 10.1038/nmeth.1865.
  PUBMED    22290181
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-01-30- 30"; volume: "9"; 
            issue: "2"; pages: "117-8"
COMMENT     Sequence Label: pCFJ356 - MCS(cxTi10816, IV)
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(167..183)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(167..183)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(190..214)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tagagggtac cagagctcac ctaggtatct agaaccggtg acgtcaccat gggaagcttc
       61 gtggatccag atatcctgca ggaattcctc gagacgtacg gtgcgcgcga tgcattcgaa
      121 gatctgccca ctagtgagtc gtattacgta gcttggcgta atcatggtca tagctgtttc
      181 ctgcccgtga cccagctttc ttgtacaaag tggctcctag ggtttccacg caagtacacg
      241 attgagctga tgggtaagaa aagaactttg cagtttggtt gtagtgaaca ataaattcgt
      301 tttttatttt ggtttacttt ttcttggttt ttgtattttt acacgggccc tactgtactc
      361 actacagtag tcacacagtt catcgactag tgtgagacaa gtgtgaaact aaacttaggc
      421 gacattttgt taataatcgt aacttaaagg aggactaaca gttcgggcga ttttgatctt
      481 atagacccaa catgggctca aatgaacgga ttgaaacttc tcgaaaactt ttcaaacttt
      541 ttcatgccgg ttcaaaattt tgaaaaagct acactgattt tagtcaaaat ctcgaatttt
      601 ggccattttt ccgtctcaca tctgtcggaa gacaaaactg ttaacaaaac tgttaatacc
      661 caactttttt aactaaaaaa aattaatatg gaaacgttgg aaacagttca ttcgatctga
      721 aaagtataaa gctaaaaaaa taagtgaatg tagaaaatct ttattttcaa agaggatcct
      781 ttctttattt acgcaacgcc actttaatct ccttcataag accgtcttgc caggaagaat
      841 ggttcgattc aattgtcttg attccgttcg ccagttctcg cattctgaaa cacattcaaa
      901 taaggatatc gtgcctacga aagacctaca
//
