LOCUS       sequence-68923-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Ailion M, Jorgensen EM
  TITLE     Improved Mos1-mediated transgenesis in C. elegans.
  JOURNAL   Nat Methods. 2012 Jan 30;9(2):117-8. doi: 10.1038/nmeth.1865.
  PUBMED    22290181
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-01-30- 30"; volume: "9"; 
            issue: "2"; pages: "117-8"
COMMENT     Sequence Label: pCFJ676 - MCS(ttTi4348, I, unc-18(+))
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    111..132
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(147..152,153..170,171..177)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    185..201
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     190..212
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     209..225
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     209..225
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
       61 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg taatgtgagt
      121 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
      181 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatgg gtcaccaggt
      241 atcgcgcttt aattagggtg caagtttttt ataattttta atcccattaa aattcaaagc
      301 agaaaggttc tgatgctcta cgcaaaatat caaaatcaca atttatgaca atattgttac
      361 aaaaatccaa aaaaaaaatt gcagttcgaa atggtgattg aagaaaacgg attacgtgaa
      421 gctactcgac gaatcatatc ctatttggaa aaatatattc atgcactgca ctatcatcat
      481 aaggatgatg agatttaata atttttgtaa tttatcttac aattagcatt ttatttaatt
      541 ttaaaaaaat ttgaatggga acataaagtc aaattcctga gctgcatggc taagggtgcc
      601 ctcgaaaatt ttccgattgt tcgatttatt tcagagaaat tcggaaaatg cgaaatcgaa
      661 aaaaagtgta gaaaatcaaa aaatgcaaaa atttatacca aaccgtaaaa aattaccaaa
      721 atttaaagat attttacgga aacttacgct tgagttattc aaacacataa ctgatattat
      781 aacattaata ctatattaat actagattaa tatgtactat agcaattttc aaaatatttt
//
