LOCUS       sequence-6893-ex        1611 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1611)
  TITLE     Collier lab plasmids
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET-22b DT 51E/148K
FEATURES             Location/Qualifiers
     source          1..1611
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             10..582
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
ORIGIN
        1 atgggcgctg atgatgttgt tgattcttct aaatcttttg tgatggaaaa cttttcttcg
       61 taccacggga ctaaacctgg ttatgtagat tccattcaaa aaggtataca aaagccaaaa
      121 tctggtacac aaggaaatta tgacgatgat tggaaagggt tttatagtac cgacaataaa
      181 tacgacgctg cgggatactc tgtagataat gaaaacccgc tctctggaaa agctggaggc
      241 gtggtcaaag tgacgtatcc aggactgacg aaggttctcg cactaaaagt ggataatgcc
      301 gaaactatta agaaagagtt aggtttaagt ctcactgaac cgttgatgga gcaagtcgga
      361 acggaagagt ttatcaaaag gttcggtgat ggtgcttcgc gtgtagtgct cagccttccc
      421 ttcgctgagg ggagttctag cgttgaatat attaataact gggaacaggc gaaagcgtta
      481 agcgtagaac ttgagattaa ttttgaaacc cgtggaaaac gtggccaaga tgcgatgtat
      541 gagtatatgg ctcaagcctg tgcaggaaat cgtgtcaggc gatcagtagg tagctcattg
      601 tcatgcataa atcttgattg ggatgtcata agggataaaa ctaagacaaa gatagagtct
      661 ttgaaagagc atggccctat caaaaataaa atgagcgaaa gtcccaataa aacagtatct
      721 gaggaaaaag ctaaacaata cctagaagaa tttcatcaaa cggcattaga gcatcctgaa
      781 ttgtcagaac ttaaaaccgt tactgggacc aatcctgtat tcgctggggc taactatgcg
      841 gcgtgggcag taaacgttgc gcaagttatc gatagcgaaa cagctgataa tttggaaaag
      901 acaactgctg ctctttcgat acttcctggt atcggtagcg taatgggcat tgcagacggt
      961 gccgttcacc acaatacaga agagatagtg gcacaatcaa tagctttatc atctttaatg
     1021 gttgctcaag ctattccatt ggtaggagag ctagttgata ttggtttcgc tgcatataat
     1081 tttgtagaga gtattatcaa tttatttcaa gtagttcata attcgtataa tcgtcccgcg
     1141 tattctccgg ggcataaaac gcaaccattt cttcatgacg ggtatgctgt cagttggaac
     1201 actgttgaag attcgataat ccgaactggt tttcaagggg agagtgggca cgacataaaa
     1261 attactgctg aaaatacccc gcttccaatc gcgggtgtcc tactaccgac tattcctgga
     1321 aagctggacg ttaataagtc caagactcat atttccgtaa atggtcggaa aataaggatg
     1381 cgttgcagag ctatagacgg tgatgtaact ttttgtcgcc ctaaatctcc tgtttatgtt
     1441 ggtaatggtg tgcatgcgaa tcttcacgtg gcatttcaca gaagcagctc ggagaaaatt
     1501 cattctaatg aaatttcatc ggattccata ggcgttcttg ggtaccagaa aacagtagat
     1561 cacaccaagg ttaattctaa gctatcgcta ttttttgaaa tcaaaagctg a
//
