LOCUS       sequence-68931-e         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TF reporter for gRNA-AAVS1_T1 (RNA-guided dTomato expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Mali P, Aach J, Stranges PB, Esvelt KM, Moosburner M, Kosuri S, Yang
            L, Church GM
  TITLE     CAS9 transcriptional activators for target specificity screening and
            paired nickases for cooperative genome engineering.
  JOURNAL   Nat Biotechnol. 2013 Aug 1. doi: 10.1038/nbt.2675.
  PUBMED    23907171
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2013 Aug 1. doi: 10.1038/nbt.2675."
COMMENT     Sequence Label: reporter-gT1
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    261..342
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(298..317)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(505..523)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(506..523)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(541..557)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(541..557)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(554..576)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    565..581
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(589..595,596..613,614..619))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    634..655
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gtacgagcgc tccgagggcc gccaccacct gttcctgtac ggcatggacg agctgtacaa
       61 gtaagaattc gagctcggta cccggggatc ctctagtcag ctgacgcgtg ctagcgcggc
      121 cgcatcgata agcttgtcga cgatatctct agaggatcat aatcagccat accacatttg
      181 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
      241 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
      301 atagcatcac aaatttcaca aataaagcat ttttttcact gcctcgagct tcctcgctca
      361 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
      421 taataaaggg cgaattccag cacactggcg gccgttacta gtggatccga gctcggtacc
      481 aagcttgatg catagcttga gtattctata gtgtcaccta aatagcttgg cgtaatcatg
      541 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
      601 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
      661 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
      721 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
      781 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
      841 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caa
//
