LOCUS       sequence-68933-e         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TF reporter for gRNA-AAVS1_T2 (RNA-guided dTomato expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Mali P, Aach J, Stranges PB, Esvelt KM, Moosburner M, Kosuri S, Yang
            L, Church GM
  TITLE     CAS9 transcriptional activators for target specificity screening and
            paired nickases for cooperative genome engineering.
  JOURNAL   Nat Biotechnol. 2013 Aug 1. doi: 10.1038/nbt.2675.
  PUBMED    23907171
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2013 Aug 1. doi: 10.1038/nbt.2675."
COMMENT     Sequence Label: reporter-gT2
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     12..28
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12..28
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        46..64
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     46..63
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     199..216
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    227..308
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     252..271
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(591..614)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(789..808)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 acaatttcac acaggaaaca gctatgacca tgattacgcc aagctattta ggtgacacta
       61 tagaatactc aagctatgca tcaagcttgg taccgagctc ggatccacta gtaacggccg
      121 ccagtgtgct ggaattcgcc ctttattacc gcctttgagt gagctgatac cgctcgccgc
      181 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag ctcgaggcag tgaaaaaaat
      241 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
      301 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
      361 aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcctctag
      421 agatatcgtc gacaagctta tcgatgcggc cgcgctagca cgcgtcagct gactagagga
      481 tccccgggta ccgagctcga attcttactt gtacagctcg tccatgccgt acaggaacag
      541 gtggtggcgg ccctcggagc gctcgtactg ttccacgatg gtgtagtcct cgttgtggga
      601 ggtgatgtcc agcttggtgt ccacgtagta gtagccgggc agttgcacgg gcttcttggc
      661 catgtagatg gtcttgaact ccaccaggta gtggccgccg tccttcagct tcagggcctg
      721 gtggatctcg cccttcagca cgccgtcgcg ggggtacagg cgctcggtgg aggcctccca
      781 gcccatggtc ttcttctgca ttacggggcc gtcgggggg
//
