LOCUS       sequence-68936-e         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 D10A+D839A+H840A+N863A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Mali P, Aach J, Stranges PB, Esvelt KM, Moosburner M, Kosuri S, Yang
            L, Church GM
  TITLE     CAS9 transcriptional activators for target specificity screening and
            paired nickases for cooperative genome engineering.
  JOURNAL   Nat Biotechnol. 2013 Aug 1. doi: 10.1038/nbt.2675.
  PUBMED    23907171
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2013 Aug 1. doi: 10.1038/nbt.2675."
COMMENT     Sequence Label: Cas9m4
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     5..29
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      117..126
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
       61 tccatagaag acaccgggac cgatccagcc tccggactct agaggatcga acccttgcca
      121 ccatggacaa gaagtactcc attgggctcg ctatcggcac aaacagcgtc ggctgggccg
      181 tcattacgga cgagtacaag gtgccgagca aaaaattcaa agttctgggc aataccgatc
      241 gccacagcat aaagaagaac ctcattggcg ccctcctgtt cgactccggg gagacggccg
      301 aagccacgcg gctcaaaaga acagcacggc gcagatatac ccgcagaaag aatcggatct
      361 gctacctgca ggagatcttt agtaatgaga tggctaaggt ggatgactct ttcttccata
      421 ggctggagga gtcctttttg gtggaggagg ataaaaagca cgagcgccac ccaatctttg
      481 gcaatatcgt ggacgaggtg gcgtaccatg aaaagtaccc aaccatatat catctgagga
      541 agaagcttgt agacagtact gataaggctg acttgcggtt gatctatctc gcgctggcgc
      601 atatgatcaa atttcgggga cacttcctca tcgaggggga cctgaaccca gacaacagcg
      661 atgtcgacaa actctttatc caactggttc agacttacaa tcagcttttc gaagagaacc
      721 cgatcaacgc atccggagtt gacgccaaag caatcctgag cgctaggctg tccaaatccc
      781 ggcggctcga aaacctcatc gcacagctcc ctggggagaa gaagaacggc ctgtttggta
      841 atcttatcgc cctgtcactc gggctgaccc ccaactttaa atctaacttc gacctggccg
      901 aa
//
