LOCUS       sequence-68938-e         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 D10A+D839A+H840A+N863A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Mali P, Aach J, Stranges PB, Esvelt KM, Moosburner M, Kosuri S, Yang
            L, Church GM
  TITLE     CAS9 transcriptional activators for target specificity screening and
            paired nickases for cooperative genome engineering.
  JOURNAL   Nat Biotechnol. 2013 Aug 1. doi: 10.1038/nbt.2675.
  PUBMED    23907171
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2013 Aug 1. doi: 10.1038/nbt.2675."
COMMENT     Sequence Label: Cas9m4
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    50..98
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     123..142
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(197..217)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggtggggtat cgacagagtg ccagccctgg gaccgaaccc cgcgtttatg aacaaacgac
       61 ccaacacccg tgcgttttat tctgtctttt tattgccgtc atagcgcggg ttccttccgg
      121 tattgtctcc ttccgtgttt cagttagcct cccccgttta aactcattac taaccggtag
      181 ggatcgaacc ctttcacacc ttcctcttct tcttggggtc agccctgctg tctccaccga
      241 gctgagagag gtcgattctt gtttcataga gccccgtaat tgactgatga atcagtgtgg
      301 cgtccaggac ctcctttgta gaggtgtacc gctttctgtc tatggtggtg tcgaagtact
      361 tgaaggctgc aggcgcgccc aagttggtca gagtaaacaa gtggataatg ttttctgcct
      421 gctccctgat gggcttatcc ctgtgcttat tgtaagcaga aagcacctta tcgaggttag
      481 cgtcggcgag gatcactctt ttggagaatt cgcttatttg ctcgatgatc tcatcaaggt
      541 agtgtttgtg ttgttccacg aacagctgct tctgctcatt atcttcggga gaccctttga
      601 gcttttcata gtggctggcc agatacaaga aattaacgta tttagagggc agtgccagct
      661 cgttaccttt ctgcagctcg cccgcactag cgagcattcg tttccggccg ttttcaagct
      721 caaagagaga gtacttggga agcttaatga tgaggtcttt tttgacctct ttatatcctt
      781 tcgcctcgag aaagtcgatg gggttttttt cgaagcttga tcgctccatg attgtgatgc
      841 ccagcagttc cttgacgctt ttgagttttt tagacttccc tttctccact ttgg
//
