LOCUS       sequence-68950-e         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Fairhead M, Krndija D, Lowe ED, Howarth M
  TITLE     Plug-And-Play Pairing Via Defined Divalent Streptavidins.
  JOURNAL   J Mol Biol. 2013 Sep 19. pii: S0022-2836(13)00600-1. doi: 
            10.1016/j.jmb.2013.09.016.
  PUBMED    24056174
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2013 Sep 19. pii: S0022-2836(13)00600-1. doi: 
            10.1016/j.jmb.2013.09.016."
COMMENT     Sequence Label: pET21-Streptavidin-Glutamate_Tag
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..74
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        55..73
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    74..98
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             113..135
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             572..589
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(642..660)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      656..703
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(827..846)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaattaatac
       61 gactcactat aggggaattg tgagcggata acaattcccc tctagaaata attttgttta
      121 actttaagaa ggagatatac atatggctga agctggtatc accggcacct ggtacaacca
      181 gctgggatcc accttcatcg ttaccgctgg tgctgacggt gctctgaccg gtacctacga
      241 atccgctgtt ggtaacgctg aatctagata cgttctgacc ggtcgttacg actccgctcc
      301 ggctaccgac ggttccggaa ccgctctggg ttggaccgtt gcttggaaaa acaactaccg
      361 taacgctcac tccgctacca cctggtctgg ccagtacgtt ggtggtgctg aagctcgtat
      421 caacacccag tggttgttga cctccggcac caccgaagct aacgcgtgga aatccaccct
      481 ggttggtcac gacaccttca ccaaagttaa accgtccgct gcttccgaag aggaagagga
      541 agagtaataa aagcttgcgg ccgcactcga gcaccaccac caccaccact gagatccggc
      601 tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc
      661 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat
      721 atccggattg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
      781 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
      841 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgg
//
