LOCUS       sequence-68954-e         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ecDHFR based destable domain function at low temperature for 
            regulating protein stability in Caenorhabditis elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Cho U, Zimmerman SM, Chen LC, Owen E, Kim JV, Kim SK, Wandless TJ
  TITLE     Rapid and Tunable Control of Protein Stability in Caenorhabditis 
            elegans Using a Small Molecule.
  JOURNAL   PLoS One. 2013 Aug 22;8(8):e72393. doi: 
            10.1371/journal.pone.0072393.
  PUBMED    23991108
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-08-22"; volume: "8(8):e72393. doi"; pages: " 
            10.1371/journal.pone.0072393"
COMMENT     Sequence Label: pBMN ltDHFR(DD)-YFP
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             153..836
                     /codon_start=1
                     /product="far-red fluorescent variant of Heteractis crispa 
                     chromoprotein"
                     /label=HcRed1
                     /note="mammalian codon-optimized"
                     /translation="MVSGLLKESMRIKMYMEGTVNGHYFKCEGEGDGNPFAGTQSMRIH
                     VTEGAPLPFAFDILAPCCEYGSRTFVHHTAEIPDFFKQSFPEGFTWERTTTYEDGGILT
                     AHQDTSLEGNCLIYKVKVHGTNFPADGPVMKNKSGGWEPSTEVVYPENGVLCGRNVMAL
                     KVGDRHLICHHYTSYRSKKAVRALTMPGFHFTDIRLQMLRKKKDEYFELYEASVARYSD
                     LPEKAN"
ORIGIN
        1 ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg
       61 tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct
      121 ttgaaaaaca cgatgataat atggccacaa ccatggtgag cggcctgctg aaggagagta
      181 tgcgcatcaa gatgtacatg gagggcaccg tgaacggcca ctacttcaag tgcgagggcg
      241 agggcgacgg caaccccttc gccggcaccc agagcatgag aatccacgtg accgagggcg
      301 cccccctgcc cttcgccttc gacatcctgg ccccctgctg cgagtacggc agcaggacct
      361 tcgtgcacca caccgccgag atccccgact tcttcaagca gagcttcccc gagggcttca
      421 cctgggagag aaccaccacc tacgaggacg gcggcatcct gaccgcccac caggacacca
      481 gcctggaggg caactgcctg atctacaagg tgaaggtgca cggcaccaac ttccccgccg
      541 acggccccgt gatgaagaac aagagcggcg gctgggagcc cagcaccgag gtggtgtacc
      601 ccgagaacgg cgtgctgtgc ggccggaacg tgatggccct gaaggtgggc gaccggcacc
      661 tgatctgcca ccactacacc agctaccgga gcaagaaggc cgtgcgcgcc ctgaccatgc
      721 ccggcttcca cttcaccgac atccggctcc agatgctgcg gaagaagaag gacgagtact
      781 tcgagctgta cgaggccagc gtggcccggt acagcgacct gcccgagaag gccaacagat
      841 ctcccgggat ggtgagcggc ctgctgaagg agagtatgcg catcaagatg tacatggagg
      901 gcaccgtgaa cggccactac ttcaagtgcg a
//
