LOCUS       sequence-68956-e         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9m3 Activator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Mali P, Aach J, Stranges PB, Esvelt KM, Moosburner M, Kosuri S, Yang
            L, Church GM
  TITLE     CAS9 transcriptional activators for target specificity screening and
            paired nickases for cooperative genome engineering.
  JOURNAL   Nat Biotechnol. 2013 Aug 1. doi: 10.1038/nbt.2675.
  PUBMED    23907171
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2013 Aug 1. doi: 10.1038/nbt.2675."
COMMENT     Sequence Label: Cas9m3-VP64
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    50..98
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     123..142
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(209..358)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(383..403)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggtggggtat cgacagagtg ccagccctgg gaccgaaccc cgcgtttatg aacaaacgac
       61 ccaacacccg tgcgttttat tctgtctttt tattgccgtc atagcgcggg ttccttccgg
      121 tattgtctcc ttccgtgttt cagttagcct cccccgttta aactcattac taaccggtag
      181 ggatcgaacc ctttcatcta gagttaatca gcatgtccag gtcgaaatca tcaagggcgt
      241 cactgccgag catgtcgagg tcaaagtcat caagggcatc cgaaccaagc atgtcaaggt
      301 caaaatcatc gagggcgtca cttcccagca tatccagatc aaaatcgtcc aatgcgtcag
      361 cccgtccgga accgctggcc tccaccttcc tcttcttctt ggggtcagcc ctgctgtctc
      421 caccgagctg agagaggtcg attcttgttt catagagccc cgtaattgac tgatgaatca
      481 gtgtggcgtc caggacctcc tttgtagagg tgtaccgctt tctgtctatg gtggtgtcga
      541 agtacttgaa ggctgcaggc gcgcccaagt tggtcagagt aaacaagtgg ataatgtttt
      601 ctgcctgctc cctgatgggc ttatccctgt gcttattgta agcagaaagc accttatcga
      661 ggttagcgtc ggcgaggatc actcttttgg agaattcgct tatttgctcg atgatctcat
      721 caaggtagtg tttgtgttgt tccacgaaca gctgcttctg ctcattatct tcgggagacc
      781 ctttgagctt ttcatagtgg ctggccagat acaagaaatt aacgtattta gagggcagtg
      841 ccagctcgtt acctttctgc agctcgcccg cactagcgag cattcgtttc cggccgtttt
      901 caagctcaaa gagagagtac ttgggaagct taatgatgag gtcttttt
//
