LOCUS       sequence-68957-e         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9m4 Activator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Mali P, Aach J, Stranges PB, Esvelt KM, Moosburner M, Kosuri S, Yang
            L, Church GM
  TITLE     CAS9 transcriptional activators for target specificity screening and
            paired nickases for cooperative genome engineering.
  JOURNAL   Nat Biotechnol. 2013 Aug 1. doi: 10.1038/nbt.2675.
  PUBMED    23907171
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2013 Aug 1. doi: 10.1038/nbt.2675."
COMMENT     Sequence Label: Cas9m4-VP64
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     5..29
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             126..146
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
       61 tccatagaag acaccgggac cgatccagcc tccggactct agaggatcga acccttgcca
      121 ccatgcccaa gaagaagagg aaggtgggaa gggggatgga caagaagtac tccattgggc
      181 tcgctatcgg cacaaacagc gtcggctggg ccgtcattac ggacgagtac aaggtgccga
      241 gcaaaaaatt caaagttctg ggcaataccg atcgccacag cataaagaag aacctcattg
      301 gcgccctcct gttcgactcc ggggagacgg ccgaagccac gcggctcaaa agaacagcac
      361 ggcgcagata tacccgcaga aagaatcgga tctgctacct gcaggagatc tttagtaatg
      421 agatggctaa ggtggatgac tctttcttcc ataggctgga ggagtccttt ttggtggagg
      481 aggataaaaa gcacgagcgc cacccaatct ttggcaatat cgtggacgag gtggcgtacc
      541 atgaaaagta cccaaccata tatcatctga ggaagaagct tgtagacagt actgataagg
      601 ctgacttgcg gttgatctat ctcgcgctgg cgcatatgat caaatttcgg ggacacttcc
      661 tcatcgaggg ggacctgaac ccagacaaca gcgatgtcga caaactcttt atccaactgg
      721 ttcagactta caatcagctt ttcgaagaga acccgatcaa
//
