LOCUS       sequence-68958-e         958 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9m4 Activator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Mali P, Aach J, Stranges PB, Esvelt KM, Moosburner M, Kosuri S, Yang
            L, Church GM
  TITLE     CAS9 transcriptional activators for target specificity screening and
            paired nickases for cooperative genome engineering.
  JOURNAL   Nat Biotechnol. 2013 Aug 1. doi: 10.1038/nbt.2675.
  PUBMED    23907171
REFERENCE   2  (bases 1 to 958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2013 Aug 1. doi: 10.1038/nbt.2675."
COMMENT     Sequence Label: Cas9m4-VP64
FEATURES             Location/Qualifiers
     source          1..958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    50..98
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     123..142
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(208..357)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(382..402)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggtggggtat cgacagagtg ccagccctgg gaccgaaccc cgcgtttatg aacaaacgac
       61 ccaacacccg tgcgttttat tctgtctttt tattgccgtc atagcgcggg ttccttccgg
      121 tattgtctcc ttccgtgttt cagttagcct cccccgttta aactcattac taaccggtag
      181 ggatcgaacc cttcatctag agttaatcag catgtccagg tcgaaatcat caagggcgtc
      241 actgccgagc atgtcgaggt caaagtcatc aagggcatcc gaaccaagca tgtcaaggtc
      301 aaaatcatcg agggcgtcac ttcccagcat atccagatca aaatcgtcca atgcgtcagc
      361 ccgtccggaa ccgctggcct ccaccttcct cttcttcttg gggtcagccc tgctgtctcc
      421 accgagctga gagaggtcga ttcttgtttc atagagcccc gtaattgact gatgaatcag
      481 tgtggcgtcc aggacctcct ttgtagaggt gtaccgcttt ctgtctatgg tggtgtcgaa
      541 gtacttgaag gctgcaggcg cgcccaagtt ggtcagagta aacaagtgga taatgttttc
      601 tgcctgctcc ctgatgggct tatccctgtg cttattgtaa gcagaaagca ccttatcgag
      661 gttagcgtcg gcgaggatca ctcttttgga gaattcgctt atttgctcga tgatctcatc
      721 aaggtagtgt ttgtgttgtt ccacgaacag ctgcttctgc tcattatctt cgggagaccc
      781 tttgagcttt tcatagtggc tggccagata caagaaatta acgtatttag agggcagtgc
      841 cagctcgtta cctttctgca gctcgcccgc actagcgagc attcgtttcc ggccgttttc
      901 aagctcaaag agagagtact tgggaagctt aatgatgagg tcttttttga cctcttta
//
