LOCUS       sequence-68966-e         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 D10A+H840A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Mali P, Aach J, Stranges PB, Esvelt KM, Moosburner M, Kosuri S, Yang
            L, Church GM
  TITLE     CAS9 transcriptional activators for target specificity screening and
            paired nickases for cooperative genome engineering.
  JOURNAL   Nat Biotechnol. 2013 Aug 1. doi: 10.1038/nbt.2675.
  PUBMED    23907171
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2013 Aug 1. doi: 10.1038/nbt.2675."
COMMENT     Sequence Label: Cas9m2
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    47..95
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     120..139
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(194..214)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggggtatcga cagagtgcca gccctgggac cgaaccccgc gtttatgaac aaacgaccca
       61 acacccgtgc gttttattct gtctttttat tgccgtcata gcgcgggttc cttccggtat
      121 tgtctccttc cgtgtttcag ttagcctccc ccgtttaaac tcattactaa ccggtaggga
      181 tcgaaccctt tcacaccttc ctcttcttct tggggtcagc cctgctgtct ccaccgagct
      241 gagagaggtc gattcttgtt tcatagagcc ccgtaattga ctgatgaatc agtgtggcgt
      301 ccaggacctc ctttgtagag gtgtaccgct ttctgtctat ggtggtgtcg aagtacttga
      361 aggctgcagg cgcgcccaag ttggtcagag taaacaagtg gataatgttt tctgcctgct
      421 ccctgatggg cttatccctg tgcttattgt aagcagaaag caccttatcg aggttagcgt
      481 cggcgaggat cactcttttg gagaattcgc ttatttgctc gatgatctca tcaaggtagt
      541 gtttgtgttg ttccacgaac agctgcttct gctcattatc ttcgggagac cctttgagct
      601 tttcatagtg gctggccaga tacaagaaat taacgtattt agagggcagt gccagctcgt
      661 tacctttctg cagctcgccc gcactagcga gcattcgttt ccggccgttt tcaagctcaa
      721 agagagagta cttgggaagc ttaatgatga ggtctttttt gacctcttta tatcctttcg
      781 cctcgagaaa gtcgatgggg tttttttcga agcttgatcg ctccatgatt gtgatgccca
      841 gcagttcctt gacgcttttg agttttttag acttcccttt ctccactttg gccacaacca
      901 gtacactgta agcgactgta ggagaatcga atccgccgta tttcttgg
//
