LOCUS       sequence-68980-e         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gram-Positive Bacterial Shuttle Vector for Nisin-Controlled 
            Inducible Expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Bryan EM, Bae T, Kleerebezem M, Dunny GM
  TITLE     Improved vectors for nisin-controlled expression in gram-positive 
            bacteria.
  JOURNAL   Plasmid. 2000 Sep;44(2):183-90.
  PUBMED    10964628
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid."; 
            date: "2000-09"; volume: "44(2)"; pages: "183-90"
COMMENT     Sequence Label: pMSP3535
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     216..233
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
       61 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      121 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      181 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      241 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcaggt
      301 taacctggct tatcgaaatt aatacgactc actataggga gaccggcctc gagtctagaa
      361 gcgctgtcga cgagctcgca tgcgaattcc tgcagcccgg gactagtggt accggatcca
      421 tgcagagtct cctgttttac aaccgggtgt acatagcgaa atacttgtaa tgcgtggtga
      481 tgcacctgaa tctttcttcg aaacagatac caaatccaag ctaaaatctt ttgtactcat
      541 tttgagtgcc tccttataat ttattttgta gttccttcga acgaaatcat tgtatctaac
      601 aaacttcaga atttaatcag agccgtttat tatgctcgcg ttatcgacaa taatattatt
      661 accaatactt tctcaagata gaattaagac tgttttagat ttgttaatgt ttctattgtc
      721 agtatagtta taagactaga tctgatccgt agcggttttc aaaatttgca accaggaatg
      781 aattactatc ccttttatca agaagcgcac aaaaagaaaa acgaaatgat acaccaatca
      841 gtgcaaaaaa agatataatg ggagataaga cggttcgtgt tcgtgctgac ttgcaccata
      901 tcataaaaat cgaaacagca aagaatggcg gaaacgtaa
//
