LOCUS       sequence-69014-e       12354 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12354)
  AUTHORS   Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S
  TITLE     A modular cloning system for standardized assembly of multigene 
            constructs.
  JOURNAL   PLoS One. 2011 Feb 18;6(2):e16765. doi: 
            10.1371/journal.pone.0016765.
  PUBMED    21364738
REFERENCE   2  (bases 1 to 12354)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011-02-18"; volume: "6(2):e16765. doi"; pages: " 
            10.1371/journal.pone.0016765"
COMMENT     Sequence Label: pAGM4673
FEATURES             Location/Qualifiers
     source          1..12354
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      6118..6127
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    8132..8156
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      8244..8954
                     /label=oriV
                     /note="incP origin of replication"
     promoter        8975..9079
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9080..9874
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      10045..10633
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10534..10553
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             10969..12117
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    12263..12287
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccttgtc ttccagtggt atggggtacc gcacggtctg ccaatccgac ggaggtttat
       61 gaattttcca ccttttccac aagctcaact agtattaacg atgtggattt agcaaaaaaa
      121 acctgtaacc ctaaatgtaa aataacgggt aagcctgcca accatgttat ggcagattaa
      181 gcgtcttttt gaagggcacc gcatctttcg cgttgccgta aatgtatccg tttagcaanv
      241 aggadnnnnn naatgaataa tccgtcgtta ctcaatcatg cggtcgaaac gatggcagtt
      301 ggctcgaaaa gttttgcgac agcctcaaag ttatttgatg caaaaacccg gcgcagcgta
      361 ctgatgctct acgcctggtg ccgccattgt gacgatgtta ttgacgatca gacgctgggc
      421 tttcaggccc ggcagcctgc cttacaaacg cccgaacaac gtctgatgca acttgagatg
      481 aaaacgcgcc aggcctatgc aggatcgcag atgcacgaac cggcgtttgc ggcttttcgg
      541 gaagtggcta tggctcatga tatcgccccg gcttacgcgt ttgatcatct ggaaggcttc
      601 gccatggatg tacgcgaagc gcaatacagc caactggatg atacgctgcg ctattgctat
      661 cacgttgcag gcgttgtcgg cttgatgatg gcgcaaatca tgggcgtgcg ggataacgcc
      721 acgctggacc gcgcctgtga ccttgggctg gcatttcagt tgaccaatat tgctcgcgat
      781 attgtggacg atgcgcatgc gggccgctgt tatctgccgg caagctggct ggagcatgaa
      841 ggtctgaaca aagagaatta tgcggcacct gaaaaccgtc aggcgctgag ccgtatcgcc
      901 cgtcgtttgg tgcaggaagc agaaccttac tatttgtctg ccacagccgg cctggcaggg
      961 ttgcccctgc gttccgcctg ggcaatcgct acggcgaagc aggtttaccg gaaaataggt
     1021 gtcaaagttg aacaggccgg tcagcaagcc tgggatcagc ggcagtcaac gaccacgccc
     1081 gaaaaattaa cgctgctgct ggccgcctct ggtcaggccc ttacttcccg gatgcgggct
     1141 catcctcccc gccctgcgca tctctggcag cgcccgctct aggcttacta nvaggadnnn
     1201 nnnaatgaaa ccaactacgg taattggtgc aggcttcggt ggcctggcac tggcaattcg
     1261 tctacaagct gcggggatcc ccgtcttact gcttgaacaa cgtgataaac ccggcggtcg
     1321 ggcttatgtc tacgaggatc aggggtttac ctttgatgca ggcccgacgg ttatcaccga
     1381 tcccagtgcc attgaagaac tgtttgcact ggcaggaaaa cagttaaaag agtatgtcga
     1441 actgctgccg gttacgccgt tttaccgcct gtgttgggag tcagggaagg tctttaatta
     1501 cgataacgat caaacccggc tcgaagcgca gattcagcag tttaatcccc gcgatgtcga
     1561 aggttatcgt cagtttctgg actattcacg cgcggtgttt aaagaaggct atctaaagct
     1621 cggtactgtc ccttttttat cgttcagaga catgcttcgc gccgcacctc aactggcgaa
     1681 actgcaggca tggagaagcg tttacagtaa ggttgccagt tacatcgaag atgaacatct
     1741 gcgccaggcg ttttctttcc actcgctgtt ggtgggcggc aatcccttcg ccacctcatc
     1801 catttatacg ttgatacacg cgctggagcg tgagtggggc gtctggtttc cgcgtggcgg
     1861 caccggcgca ttagttcagg ggatgataaa gctgtttcag gatctgggtg gcgaagtcgt
     1921 gttaaacgcc agagtcagcc atatggaaac gacaggaaac aagattgaag ccgtgcattt
     1981 agaggacggt cgcaggttcc tgacgcaagc cgtcgcgtca aatgcagatg tggttcatac
     2041 ctatcgcgac ctgttaagcc agcaccctgc cgcggttaag cagtccaaca aactgcagac
     2101 taagcgcatg agtaactctc tgtttgtgct ctattttggt ttgaatcacc atcatgatca
     2161 gctcgcgcat cacacggttt gtttcggccc gcgttaccgc gagctgattg acgaaatttt
     2221 taatcatgat ggcctcgcag aggacttctc actttatctg cacgcgccct gtgtcacgga
     2281 ttcgtcactg gcgcctgaag gttgcggcag ttactatgtg ttggcgccgg tgccgcattt
     2341 aggcaccgcg aacctcgact ggacggttga ggggccaaaa ctacgcgacc gtatttttgc
     2401 gtaccttgag cagcattaca tgcctggctt acggagtcag ctggtcacgc accggatgtt
     2461 tacgccgttt gattttcgcg accagcttaa tgcctatcat ggctcagcct tttctgtgga
     2521 gcccgttctt acccagagcg cctggtttcg gccgcataac cgcgataaaa ccattactaa
     2581 tctctacctg gtcggcgcag gcacgcatcc cggcgcaggc attcctggcg tcatcggctc
     2641 ggcaaaagcg acagcaggtt tgatgctgga ggatctgata tgagctttta cnvaggadnn
     2701 nnnnaatgca accgcattat gatctgattc tcgtgggggc tggactcgcg aatggcctta
     2761 tcgccctgcg acttcagcag cagcaacctg atatgcgtat tttgcttatc gacgccgcac
     2821 cccaggcggg cgggaatcat acgtggtcat ttcaccacga tgatttgact gagagccaac
     2881 atcgttggat agctccgctg gtggttcatc actggcccga ctatcaggta cgctttccca
     2941 cacgccgtcg taagctgaac agcggctact tttgtattac ttctcagcgt ttcgctgagg
     3001 ttttacagcg acagtttggc ccgcacttgt ggatggatac cgcggtcgca gaggttaatg
     3061 cggaatctgt tcggttgaaa aagggtcagg ttatcggtgc ccgcgcggtg attgacgggc
     3121 ggggttatgc ggcaaattca gcactgagcg tgggcttcca ggcgtttatt ggccaggaat
     3181 ggcgattgag tcacccgcat ggtttatcat ctccgattat catggatgcc acggtcgatc
     3241 agcaaaatgg ttatcgcttc gtgtacagcc tgccgctctc gccgaccaga ttgttaattg
     3301 aagatacgca ctatattgat aatgcgacat tagatcctga atgcgcgcgg caaaatattt
     3361 gcgactatgc cgcgcaacag ggttggcagc ttcagacact gctgcgagaa gaacagggcg
     3421 ccttacccat tactctgtcg ggcaatgccg acgcattctg gcagcagcgc cccctggcct
     3481 gtagtggatt acgtgccggt ctgttccatc ctaccaccgg ctattcactg ccgctggcgg
     3541 ttgccgtggc cgaccgcctg agtgcacttg atgtctttac gtcggcctca attcaccatg
     3601 ccattacgca ttttgcccgc gagcgctggc agcagcaggg ctttttccgc atgctgaatc
     3661 gcatgctgtt tttagccgga cccgccgatt cacgctggcg ggttatgcag cgtttttatg
     3721 gtttacctga agatttaatt gcccgttttt atgcgggaaa actcacgctg accgatcggc
     3781 tacgtattct gagcggcaag ccgcctgttc cggtattagc agcattgcaa gccattatga
     3841 cgactcatcg ttaagcttca ganvaggadn nnnnnaatga gttttgatat tgccaaatac
     3901 ccgaccctgg cactggtcga ctccacccag gagttacgac tgttgccgaa agagagttta
     3961 ccgaaactct gcgacgaact gcgccgctat ttactcgaca gcgtgagccg ttccagcggg
     4021 cacttcgcct ccgggctggg cacggtcgaa ctgaccgtgg cgctgcacta tgtctacaac
     4081 accccgtttg accaattgat ttgggatgtg gggcatcagg cttatccgca taaaattttg
     4141 accggacgcc gcgacaaaat cggcaccatc cgtcagaaag gcggtctgca cccgttcccg
     4201 tggcgcggcg aaagcgaata tgacgtatta agcgtcgggc attcatcaac ctccattagt
     4261 gccggaattg gtattgcggt tgctgccgaa aaagaaggca aaaatcgccg caccgtctgt
     4321 gtcattggcg atggcgcgat taccgcaggc atggcgtttg aagcgatgaa tcacgcgggc
     4381 gatatccgtc ctgatatgct ggtgattctc aacgacaatg aaatgtcgat ttccgaaaat
     4441 gtcggcgcgc tcaacaacca tctggcacag ctgctttccg gtaagcttta ctcttcactg
     4501 cgcgaaggcg ggaaaaaagt tttctctggc gtgccgccaa ttaaagagct gctcaaacgc
     4561 accgaagaac atattaaagg catggtagtg cctggcacgt tgtttgagga gctgggcttt
     4621 aactacatcg gcccggtgga cggtcatgat gtgctggggc ttatcaccac gctaaagaac
     4681 atgcgcgacc tgaaaggccc gcagttcctg catatcatga ccaaaaaagg tcgtggttat
     4741 gaaccggcag aaaaagaccc gatcactttc catgccgtgc ctaaatttga tccctccagc
     4801 ggttgtttgc cgaaaagtag cggcggtttg ccgagctatt caaaaatctt tggcgactgg
     4861 ttgtgcgaaa cggcagcgaa agacaacaag ctgatggcga ttactccggc gatgcgtgaa
     4921 ggttccggca tggtcgagtt ttcacgtaaa ttcccggatc gctacttcga cgtggcaatt
     4981 gccgagcaac atgcggtgac ctttgctgcg ggtctggcga ttggtgggta caaacccatt
     5041 gtcgcgattt actccacttt cctgcaacgc gcctatgatc aggtgctgca tgacgtggcg
     5101 attcaaaagc ttccggtcct gttcgccatc gaccgcgcgg gcattgttgg tgctgacggt
     5161 caaacccatc agggtgcttt tgatctctct tacctgcgct gcataccgga aatggtcatt
     5221 atgaccccga gcgatgaaaa cgaatgtcgc cagatgctct ataccggcta tcactataac
     5281 gatggcccgt cagcggtgcg ctacccgcgt ggcaacgcgg tcggcgtgga actgacgccg
     5341 ctggaaaaac taccaattgg caaaggcatt gtgaagcgtc gtggcgagaa actggcgatc
     5401 cttaactttg gtacgctgat gccagaagcg gcgaaagtcg ccgaatcgct gaacgccacg
     5461 ctggtcgata tgcgttttgt gaaaccgctt gatgaagcgt taattctgga aatggccgcc
     5521 agccatgaag cgctggtcac cgtagaagaa aacgccatta tgggcggcgc aggcagcggc
     5581 gtgaacgaag tgctgatggc ccatcgtaaa ccagtacccg tgctgaacat tggcctgccg
     5641 gacttcttta ttccgcaagg aactcaggaa gaaatgcgcg ccgaactcgg cctcgatgcc
     5701 gctggtatgg aagccaaaat caaggcctgg ctggcataag ctttgtgnva ggadnnnnnn
     5761 aatgagcgca catgccctgc ccaaggcaga tctgaccgcc accagcctga tcgtatcggg
     5821 cggcatcatc gccgcttggc tggccctgca tgtgcatgcg ctgtggtttc tggacgcagc
     5881 ggcgcatccc atcctggcga tcgcaaattt cctggggctg acctggctgt cggtcggatt
     5941 gttcatcatc gcgcatgacg cgatgcacgg gtcggtggtg ccggggcgtc cgcgcgccaa
     6001 tgcggcgatg ggccagcttg tcctgtggct gtatgccgga ttttcgtggc gcaagatgat
     6061 cgtcaagcac atggcccatc accgccatgc cggaaccgac gacgaccccg atttcgacca
     6121 tggcggcccg gtccgctggt acgcccgctt catcggcacc tatttcggct ggcgcgaggg
     6181 gctgcttctg cccgtcatcg tgacggtcta tgcgctgatc cttggggatc gctggatgta
     6241 cgtggtattc tggccgctgc cgtcgatcct ggcgtcgatc cagctgttcg tgttcggcac
     6301 ctggctgccg caccgccccg gccacgacgc gttcccggac cgccacaatg cgcggtcgtc
     6361 gcggatcagc gaccccgtgt cgctgctgac ctgttttcac tttggcggtt atcatcacga
     6421 acaccacctg cacccgacgg tgccgtggtg gcgcctgccc agcacccgca ccaagggcga
     6481 caccgcataa gcttgagcnv aggadnnnnn naatgcaaac ggaacacgtc attttattga
     6541 atgcacaggg agttcccacg ggtacgctgg aaaagtatgc cgcacacacg gcagacaccc
     6601 gcttacatct cgcgttctcc agttggctgt ttaatgccaa aggacaatta ttagttaccc
     6661 gccgcgcact gagcaaaaaa gcatggcctg gcgtgtggac taactcggtt tgtgggcacc
     6721 cacaactggg agaaagcaac gaggacgcag tgatccgccg ttgccgttat gagcttggcg
     6781 tggaaattac gcctcctgaa tctatctatc ctgactttcg ctaccgcgcc accgatccga
     6841 gtggcattgt ggaaaatgaa gtgtgtccgg tatttgccgc acgcaccact agtgcgttac
     6901 agatcaatga tgatgaagtg atggattatc aatggtgtga tttagcagat gtattacacg
     6961 gtattgatgc aacgccgtgg gcgttcagtc cgtggatggt gatgcaggcg acaaatcgcg
     7021 aagccagaaa acgattatct gcatttaccc agcttaaata agctttgccn vaggadnnnn
     7081 nnaatgacgg tctgcgcaaa aaaacacgtt catctcactc gcgatgctgc ggagcagtta
     7141 ctggctgata ttgatcgacg ccttgatcag ttattgcccg tggagggaga acgggatgtt
     7201 gtgggtgccg cgatgcgtga aggtgcgctg gcaccgggaa aacgtattcg ccccatgttg
     7261 ctgttgctga ccgcccgcga tctgggttgc gctgtcagcc atgacggatt actggatttg
     7321 gcctgtgcgg tggaaatggt ccacgcggct tcgctgatcc ttgacgatat gccctgcatg
     7381 gacgatgcga agctgcggcg cggacgccct accattcatt ctcattacgg agagcatgtg
     7441 gcaatactgg cggcggttgc cttgctgagt aaagcctttg gcgtaattgc cgatgcagat
     7501 ggcctcacgc cgctggcaaa aaatcgggcg gtttctgaac tgtcaaacgc catcggcatg
     7561 caaggattgg ttcagggtca gttcaaggat ctgtctgaag gggataagcc gcgcagcgct
     7621 gaagctattt tgatgacgaa tcactttaaa accagcacgc tgttttgtgc ctcgatgcag
     7681 atggcctcga ttgttgcgaa tgcctccagc gaagcgcgtg attgcctgca tcgtttttca
     7741 cttgatcttg gtcaggcatt tcaactgctg gacgatttga ccgatggcat gaccgacacc
     7801 ggtaaggata gcaatcagga cgccggtaaa tcgacgctgg tcaatctgtt aggcccgagg
     7861 gcggttgaag aacgtctgag acaacatctt cagcttgcca gtgagcatct ctctgcggcc
     7921 tgccaacacg ggcacgccac tcaacatttt attcaggcct ggtttgacaa aaaactcgct
     7981 gccgtcagtt aagcttgcaa gaattcaagc ttggagtacg ttaattagtt gtctgtttta
     8041 agccccgtgc tctccgccgg ggctttttta ttaaaaacca ctcaagtgga agacaaggga
     8101 cacgaagtga tccgtttaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa
     8161 gagaaaagag cgtttattag aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg
     8221 ttcgtccatt tgtatgtgcc agccgccttt gcgacgctca ccgggctggt tgccctcgcc
     8281 gctgggctgg cggccgtcta tggccctgca aacgcgccag aaacgccgtc gaagccgtgt
     8341 gcgagacacc gcggccgccg gcgttgtgga tacctcgcgg aaaacttggc cctcactgac
     8401 agatgagggg cggacgttga cacttgaggg gccgactcac ccggcgcggc gttgacagat
     8461 gaggggcagg ctcgatttcg gccggcgacg tggagctggc cagcctcgca aatcggcgaa
     8521 aacgcctgat tttacgcgag tttcccacag atgatgtgga caagcctggg gataagtgcc
     8581 ctgcggtatt gacacttgag gggcgcgact actgacagat gaggggcgcg atccttgaca
     8641 cttgaggggc agagtgctga cagatgaggg gcgcacctat tgacatttga ggggctgtcc
     8701 acaggcagaa aatccagcat ttgcaagggt ttccgcccgt ttttcggcca ccgctaacct
     8761 gtcttttaac ctgcttttaa accaatattt ataaaccttg tttttaacca gggctgcgcc
     8821 ctgtgcgcgt gaccgcgcac gccgaagggg ggtgcccccc cttctcgaac cctcccggcc
     8881 cgctaacgcg ggcctcccat ccccccaggg gctgcgcccc tcggccgcga acggcctcac
     8941 cccaaaaatg gcagcgctgg ccaattcccg agtgcgcgga acccctattt gtttattttt
     9001 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     9061 atattgaaaa aggaagagta tggctaaaat gagaatatca ccggaattga aaaaactgat
     9121 cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg tatataagct
     9181 ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata aagggaccac
     9241 ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc tgcctgttcc
     9301 aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga gtgaggccga
     9361 tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct
     9421 gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc cctatacgaa
     9481 tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc tggccgatgt
     9541 ggattgcgaa aactgggaag aggacactcc atttaaagat ccgcgcgagc tgtatgattt
     9601 tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc tgggagacag
     9661 caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga gaagcggcag
     9721 ggcggacaag tggtatgaca ttgccttctg cgtccggtcg ctcagggagg atatcgggga
     9781 agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt gggagaaaat
     9841 aaaatattat attttactgg atgaattgtt ttagctgtca gaccaagttt actcatatat
     9901 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     9961 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
    10021 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
    10081 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
    10141 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
    10201 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
    10261 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
    10321 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
    10381 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
    10441 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
    10501 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
    10561 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
    10621 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcag atcctagatg
    10681 tggcgcaacg atgccggcga caagcaggag cgcaccgact tcttccgcat caagtgtttt
    10741 ggctctcagg ccgaggccca cggcaagtat ttgggcaagg ggtcgctggt attcgtgcag
    10801 ggcaagattc ggaataccaa gtacgagaag gacggccaga cggtctacgg gaccgacttc
    10861 attgccgata aggtggatta tctggacacc aaggcaccag gcgggtcaaa tcaggaataa
    10921 gggcacattg ccccggcgtg agtcggggca atcccgcaag gagggtgaat gaatcggacg
    10981 tttgaccgga aggcatacag gcaagaactg atcgacgcgg ggttttccgc cgaggatgcc
    11041 gaaaccatcg caagccgcac cgtcatgcgt gcgccccgcg aaaccttcca gtccgtcggc
    11101 tcgatggtcc agcaagctac ggccaagatc gagcgcgaca gcgtgcaact ggctccccct
    11161 gccctgcccg cgccatcggc cgccgtggag cgttcgcgtc gtcttgaaca ggaggcggca
    11221 ggtttggcga agtcgatgac catcgacacg cgaggaacta tgacgaccaa gaagcgaaaa
    11281 accgccggcg aggacctggc aaaacaggtc agcgaggcca agcaggccgc gttgctgaaa
    11341 cacacgaagc agcagatcaa ggaaatgcag ctttccttgt tcgatattgc gccgtggccg
    11401 gacacgatgc gagcgatgcc aaacgacacg gcccgctctg ccctgttcac cacgcgcaac
    11461 aagaaaatcc cgcgcgaggc gctgcaaaac aaggtcattt tccacgtcaa caaggacgtg
    11521 aagatcacct acaccggcgt cgagctgcgg gccgacgatg acgaactggt gtggcagcag
    11581 gtgttggagt acgcgaagcg cacccctatc ggcgagccga tcaccttcac gttctacgag
    11641 ctttgccagg acctgggctg gtcgatcaat ggccggtatt acacgaaggc cgaggaatgc
    11701 ctgtcgcgcc tacaggcgac ggcgatgggc ttcacgtccg accgcgttgg gcacctggaa
    11761 tcggtgtcgc tgctgcaccg cttccgcgtc ctggaccgtg gcaagaaaac gtcccgttgc
    11821 caggtcctga tcgacgagga aatcgtcgtg ctgtttgctg gcgaccacta cacgaaattc
    11881 atatgggaga agtaccgcaa gctgtcgccg acggcccgac ggatgttcga ctatttcagc
    11941 tcgcaccggg agccgtaccc gctcaagctg gaaaccttcc gcctcatgtg cggatcggat
    12001 tccacccgcg tgaagaagtg gcgcgagcag gtcggcgaag cctgcgaaga gttgcgaggc
    12061 agcggcctgg tggaacacgc ctgggtcaat gatgacctgg tgcattgcaa acgctagggc
    12121 cttgtggggt cagttccggc tgggggttca gcagcccctg ctcggatctg ttggaccgga
    12181 cagtagtcat ggttgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt
    12241 cggggagctg ttggctggct ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta
    12301 gacaacttaa taacacattg cggacgtttt taatgtactg gggttgaaca ctct
//
