LOCUS       sequence-69034-e       10988 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10988)
  AUTHORS   Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S
  TITLE     A modular cloning system for standardized assembly of multigene 
            constructs.
  JOURNAL   PLoS One. 2011 Feb 18;6(2):e16765. doi: 
            10.1371/journal.pone.0016765.
  PUBMED    21364738
REFERENCE   2  (bases 1 to 10988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011-02-18"; volume: "6(2):e16765. doi"; pages: " 
            10.1371/journal.pone.0016765"
COMMENT     Sequence Label: pICH84000
FEATURES             Location/Qualifiers
     source          1..10988
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    50..74
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(230..1378)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(1714..2302)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1794..1813)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2473..3264,3265..3333))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3096..3115
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3334..3438)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3525..4156
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    4248..4272
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     regulatory      5279..5288
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtggcgtctc agggattgtc ttcacgaagt ggtttaaact atcagtgttt gacaggatat
       61 attggcgggt aaacctaaga gaaaagagcg tttattagaa taatcggata tttaaaaggg
      121 cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg ccaaccacag ggttccccag
      181 atcaggcgct ggctgctgaa cccccagccg gaactgaccc cacaaggccc tagcgtttgc
      241 aatgcaccag gtcatcattg acccaggcgt gttccaccag gccgctgcct cgcaactctt
      301 cgcaggcttc gccgacctgc tcgcgccact tcttcacgcg ggtggaatcc gatccgcaca
      361 tgaggcggaa ggtttccagc ttgagcgggt acggctcccg gtgcgagctg aaatagtcga
      421 acatccgtcg ggccgtcggc gacagcttgc ggtacttctc ccatatgaat ttcgtgtagt
      481 ggtcgccagc aaacagcacg acgatttcct cgtcgatcag gacctggcaa cgggacgttt
      541 tcttgccacg gtccaggacg cggaagcggt gcagcagcga caccgattcc aggtgcccaa
      601 cgcggtcgga cgtgaagccc atcgccgtcg cctgtaggcg cgacaggcat tcctcggcct
      661 tcgtgtaata ccggccattg atcgaccagc ccaggtcctg gcaaagctcg tagaacgtga
      721 aggtgatcgg ctcgccgata ggggtgcgct tcgcgtactc caacacctgc tgccacacca
      781 gttcgtcatc gtcggcccgc agctcgacgc cggtgtaggt gatcttcacg tccttgttga
      841 cgtggaaaat gaccttgttt tgcagcgcct cgcgcgggat tttcttgttg cgcgtggtga
      901 acagggcaga gcgggccgtg tcgtttggca tcgctcgcat cgtgtccggc cacggcgcaa
      961 tatcgaacaa ggaaagctgc atttccttga tctgctgctt cgtgtgtttc agcaacgcgg
     1021 cctgcttggc ctcgctgacc tgttttgcca ggtcctcgcc ggcggttttt cgcttcttgg
     1081 tcgtcatagt tcctcgcgtg tcgatggtca tcgacttcgc caaacctgcc gcctcctgtt
     1141 caagacgacg cgaacgctcc acggcggccg atggcgcggg cagggcaggg ggagccagtt
     1201 gcacgctgtc gcgctcgatc ttggccgtag cttgctggac catcgagccg acggactgga
     1261 aggtttcgcg gggcgcacgc atgacggtgc ggcttgcgat ggtttcggca tcctcggcgg
     1321 aaaaccccgc gtcgatcagt tcttgcctgt atgccttccg gtcaaacgtc cgattcattc
     1381 accctccttg cgggattgcc ccgactcacg ccggggcaat gtgcccttat tcctgatttg
     1441 acccgcctgg tgccttggtg tccagataat ccaccttatc ggcaatgaag tcggtcccgt
     1501 agaccgtctg gccgtccttc tcgtacttgg tattccgaat cttgccctgc acgaatacca
     1561 gcgacccctt gcccaaatac ttgccgtggg cctcggcctg agagccaaaa cacttgatgc
     1621 ggaagaagtc ggtgcgctcc tgcttgtcgc cggcatcgtt gcgccacatc taggatctgc
     1681 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     1741 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     1801 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     1861 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     1921 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     1981 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     2041 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     2101 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     2161 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     2221 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     2281 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     2341 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     2401 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     2461 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     2521 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     2581 accatctggc cccagtgctg caatgatacc gcgagaacca cgctcaccgg ctccagattt
     2641 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     2701 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     2761 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     2821 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     2881 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     2941 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     3001 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     3061 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     3121 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     3181 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     3241 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     3301 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     3361 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     3421 acaaataggg gttccgcgca cgaattggcc agcgctgcca tttttggggt gaggccgttc
     3481 gcggccgagg ggcgcagccc ctggggggat gggaggcccg cgttagcggg ccgggagggt
     3541 tcgagaaggg ggggcacccc ccttcggcgt gcgcggtcac gcgcacaggg cgcagccctg
     3601 gttaaaaaca aggtttataa atattggttt aaaagcaggt taaaagacag gttagcggtg
     3661 gccgaaaaac gggcggaaac ccttgcaaat gctggatttt ctgcctgtgg acagcccctc
     3721 aaatgtcaat aggtgcgccc ctcatctgtc agcactctgc ccctcaagtg tcaaggatcg
     3781 cgcccctcat ctgtcagtag tcgcgcccct caagtgtcaa taccgcaggg cacttatccc
     3841 caggcttgtc cacatcatct gtgggaaact cgcgtaaaat caggcgtttt cgccgatttg
     3901 cgaggctggc cagctccacg tcgccggccg aaatcgagcc tgcccctcat ctgtcaacgc
     3961 cgcgccgggt gagtcggccc ctcaagtgtc aacgtccgcc cctcatctgt cagtgagggc
     4021 caagttttcc gcgaggtatc cacaacgccg gcggccgcgg tgtctcgcac acggcttcga
     4081 cggcgtttct ggcgcgtttg cagggccata gacggccgcc agcccagcgg cgagggcaac
     4141 cagcccggtg agcgtcgcaa aggagatcct gatctgactg atgggctgcc tgtatcgagt
     4201 ggtgattttg tgccgagctg ccggtcgggg agctgttggc tggctggtgg caggatatat
     4261 tgtggtgtaa acaaattgac gcttagacaa cttaataaca cattgcggac gtttttaatg
     4321 tactggggtg gatgcagtgg gccccactct gtgaagacaa tgtgtgagac gccagtggta
     4381 tggggtaccg cacggtctgc caatccgacg gaggtttatg aattttccac cttttccaca
     4441 agctcaacta gtattaacga tgtggattta gcaaaaaaaa cctgtaaccc taaatgtaaa
     4501 ataacgggta agcctgccaa ccatgttatg gcagattaag cgtctttttg aagggcaccg
     4561 catctttcgc gttgccgtaa atgtatccgt ttagcaanva ggadnnnnnn aatgagcacg
     4621 tattctgaca tttgcatcgt tggcgccggc ataggaggct tgacttgcgc caacaacctg
     4681 atcgacgccg ccgccggcag gaacctgcgc atccgcgtat tcgacctgaa tgccaccgta
     4741 ggcggccgca tccagtcgcg gaaaatagat ggcgaggaaa tcgccgaact cggcgccgcc
     4801 cgctactcgc cgcagctgca tccgcatttc cagcaactca tgcagggcag cggcttgccg
     4861 catgcggtct acccgttcac cgaggtcatc tcccacgata gcgtgctgga ggagctgaag
     4921 gcaacgctgg atgagctgag cccgatgctg aaaatgcatc cgaacgactc cttcctcgag
     4981 ttcgtcagcc attacctggg cgccgccaag gccagccaca tcatcaaggc gaccggctat
     5041 gacgccctgc tgctgccgat ggtgtcggcg gccatggcct acgacatcat caagaagcac
     5101 ccggaaacgc agcactttac ggaaaacgcc gccaaccagt ggcactacgc caccgacggc
     5161 taccacgaat tgctgtgcca gttgcagcac caggcccagg tcgccggggt ggaattcagg
     5221 ctcgaacacc gcttgctgtc cgttgaaaaa tcgggcgccg accatgtgct cgccttcagc
     5281 caccatggcg acacgcagat gcaccgcacg cgccatctgg tgatggccat cccgccgtcc
     5341 gccatgccgc gcctgaacct ggatttcccg aacgcctgga gtccgttcca atacgactcg
     5401 ctgcccctgt tcaagggatt cttcacgttc gacacagcct ggtgggatgc gctggggctg
     5461 accgacaagg tgctgatggc ggcaaatccc ctgcgcaaga tctacttcaa gagcgacaaa
     5521 tacgtgctgt tctacaccga cagcaaaagc gccacctact ggcgggacag cctggagctt
     5581 ggtgaggacg tatacctgga gcgtgtccgc agccacctgg aagaagtcct gccgctcgat
     5641 ggccagcctc tgccgcagat caaggcgcac ttccacaagt tctggccgca tggcgtcgag
     5701 ttttgcgtgg agccggaagc cgaccacccg gccatcctgc tgcaccggga cggcatcatc
     5761 tcctgctcgg atgcctatac cgcgcattgc ggctggatgg aaggcagcct gatcagcgcc
     5821 cagcacgcca gcggcctgtt gctgcagcgc ctcgatcaac ggacggaaga agaagctgcc
     5881 aacgatacct tcatcacttc ctcgaccgag cgcgcatgag cttactanva ggadnnnnnn
     5941 aatgagccta cttgacttcc cccgcctgca ttttcggggt tttgcccgcg ccaatgtgcc
     6001 gacggggaat cgcaatacgc acggcaacat cgatatcgcc acgaatgcgg tatcgatggc
     6061 gggcgaggct gtcgacctga gccggccgcc agccgaattc catgcgcacc tgaaacagct
     6121 cgccccccgc ttcaacgcac agggcaagcc cgatccggac ggcatcttca gccaggcgac
     6181 aggctataat ttttgcggga acaaccattt ctcgtgggaa aacgcgcgga tcacgggcgt
     6241 ccagttgcgt gatggcgagg tcgataccca ggacgcgctg gtgggcgcca agctgggcct
     6301 gtggggccac tacaacgagt acctgcgcac gacgttcaac cgcgcaaggt ggatcgacaa
     6361 caacccggcg cagcccgaca ccacgctgat ctacgcgggc cagttcacct tgagcgacaa
     6421 gctggccacg cccaatacgc ccacgctgtt cacggccgac atcgcgcagg cgcactcggt
     6481 acgctggctc ggcagcggcc acatcacgga acgcagcggg catttcctgg acgaggaatt
     6541 cggccgctcc aggctgttcc agttttccgt ggccaagcag gacccgcatt tcctgttcaa
     6601 tccggacctg ccgctgccgg ccagtatgca tgccttgcag caagccctgg ccgacgacga
     6661 ggtgctgggc ctgacggtgc aatactgcct gttcaatatg tcgacgccgc aaaaacccga
     6721 ttcgcccgtg ttctacgacc tggccggcag catcggcctg tggcggcgcg gcgagctggc
     6781 cacctatccg gccggccgcc tgctgcagcc gcgccagggc agcctggggc cggtgctggt
     6841 gaaagtgcat gcggatcgcg tgagtttcaa catgcctacc gcaatcccgt ttactacccg
     6901 tgatgccggg gctgtatccg agcagcatcc gacacatgcg ctgggtggta aacaagcatt
     6961 gggcgatctg ctgctgcacg atggggcagg gaccgtactc gcccgtattc cggaacagtt
     7021 gtatcgcgac tattggcgtc atcatggcgt gtttgatgtt ccattacagc atgcgggagc
     7081 tgcaccaggt tctctttcgc tcggttcagc ccaggcacag tgggatgaag ccgattgggt
     7141 cctccaaagc gattctaacc agctgtacct ggaggcaccg aatcgcaaca aacacgagca
     7201 gtttccgcag accatcacgg ttcaatcgcg tttccgtggt gaactggctg cacctccgtc
     7261 actggccgaa gcggaagatg gcgtcctctt agccgtcgag cagcaaccga gtccgttagg
     7321 ccacggctat accacgctga ccctcacagg tcgtaaaccg ggagcaacgc gcattgtcct
     7381 tggaactggc aaagcgaaac agtatcttgg cgtgcgggtt ctgccggatg actgggactt
     7441 agacgacgtt ccagcagaac aagtggacta tgccttcttg taccgccatg tgatgagtta
     7501 ctacgaattg gtttacccct ttatgtccga taaggtgttc agcctggctg atcagtgcaa
     7561 atgcgaaacg tattcacgcc tgatgtggca gatgtgtgac ccgcaaaatc gggacaaaag
     7621 ctactacatg cccagcactc gcgaactgtc gctgcctaaa tcccgcttgt ttctgaaata
     7681 tctgacccaa gtagaagccg ccgcggccgc aaaagcggcg gctccggaac cagcggcgcc
     7741 tcatgcgatt ggcggcaaag ctgaactgat agatgagctg aagaaggcga ttgacctgga
     7801 attaagcctg atgctgcagt atctgtacgc tgcgtatagc atcccgaatt atgcgcaagg
     7861 tgccgcgttg gtgcagtctg gtcgctggct tcccgcggaa ctggagttag cgtgtggcgc
     7921 cgaggatcgg cgccgcaaca gcggcacgcg cggcgcgctg ctggaaatcg cccatgaaga
     7981 aatgattcac tacttattgg tgaacaatgt attgatggcg cttggcgaac cgttttacag
     8041 cggtaccccg ctgctgggcc agcaggcgcg ccagcgtttc ggcctggaca cggaatttgc
     8101 gttcgaacca ttttccgaac acgtgctggc ccgcttcgtg cgttttgaat ggcccgacta
     8161 cattcccacg ccgggcaaat ccatcgccac cttctatatc gcgatccgcc aggccctggc
     8221 cgagctgccc ggcctgttcg aaagcggcgg cggcaagcgc ggcggcgagc accacctgtt
     8281 cctgaaagaa ctgaccaacc gcgcctatcc cggctaccag ctggaagtat ccgaccgcga
     8341 cagcgcgttg ttcgccatcg atttcgtcac ggaacagggc gaaggcgtgg ccgtcgattc
     8401 gccgcatttc gcctcctcgc acttccagcg gctgcgcgcc atcgccggca ggttttcggc
     8461 ctgcgacaag ccgttcgaac cggcgctgcc ggcgctgaag aatcccgtgc tggaagcgcg
     8521 cgcggactgc agcgtggtga ccgatcagaa ggcgcgcgcg ctgatgcagc tgtatcaggg
     8581 ctgctatgaa ctgaccttcc tgatgatggc gcaccatttt gcgcagcagc cgctgggcag
     8641 cctgcgccgc tcgcgcctga tgaacgcgtc catcgacatc atgacaggcc tgttgcgccc
     8701 cctgtcggcg gccctgatga acatgccgtc cggcctgcct ggccgccatg ctggaccgcc
     8761 cgtgcccgag ccggtcagca gccgggtcag cagcgactac agcctgggct gcgacatgct
     8821 ggcgcagaga tgcctggcgc tggcgcagta cgcgcgcagc ctggagagcg atgctatcgg
     8881 catggcgccg atagaaatgt tggagttttt taatcagcaa cttaccgatt tatctcgggg
     8941 aaagatgtca agagaggctt gagcttttac nvaggadnnn nnnaatgccg acacacgtca
     9001 gcccgccgct gctgccgatg caatggagca gcgcctatgt ttcctactgg acgccgatgc
     9061 aggcggatga ccaggtcacc tccggctatt gctggttcga ctatgcgcgc aatatctgcc
     9121 gcatcgacgg cctgttcaac ccctggtcgg aaaaggaaca tggacacctg ctgtggatgt
     9181 cggaaatcgg cgacgccagg cgcgaacaaa gccgcaagca gaaagtggcc tacgcaaggc
     9241 aagcggaggc ggctggcgag cagctgcagg gcacggcgct ggccgatgag gtgaccccgt
     9301 tccatgagct gttcctgccg caggcggtgc tgctggacgg cggtgcccgt cacgacggcc
     9361 gccataccgt gctgggccgg gaggcggacg cctgggtagt cgagcgggcg ggcaagccgc
     9421 catcggtctt ttacctggag gccggtggca accgcctgct gcgcatggtc accggcaatg
     9481 acccgcagca cctgtcggta cgcgactttc ccaacctgtt tgtcagcgac attccggaca
     9541 gcgtctttac gtcttgcaac acctgagctt caganvagga dnnnnnnaat gcataaaatc
     9601 attatcgtcg gcggaggcct ggcaggcagc ctcagcgcta tttatctggc gcaacggggg
     9661 cacgatgtcc acgttgtcga aaagcgcggc gatccgctgc tggagaatgc cgcaaacgcc
     9721 gaccccgtca actcgcgcgc catcggcgtg agcatgacgg tacgcggcat caaggccgtc
     9781 ctggccgccg gcatcagcaa gcaggagctc gaccagtgcg gcgaacccat cgtcggcatg
     9841 gcattcagcg tgggcggccg gcaccggata cgcgagctga ccccgctcga aggcctgttc
     9901 cccctgtcgc tggaccgcac cgccttccag cgcctgctga accggcatgc cgccctgcat
     9961 gaggtgaagt attactttga gcataaatgc ctggatgtcg acctggaaag aaagatcgtg
    10021 ctgatccagg gcccggacgg cgccttgcag aagctgcatg gcgacctggt cattggcgcc
    10081 gacggcgccc actctgccgt gcggcgcgcc atgcaaagcg gcatgcgccg tttcgagttc
    10141 aggcaaagtt acttccgcca cggctacaag acgctggtgt tgccgaacgc ggcggatctg
    10201 ggtttcagga aggatttgct gtacttcttc ggcatggatt ccaagggcct gtttgccggc
    10261 cgcgcggcca ccatcccgga cggcagcatc agctttgccc tgtgcctgcc ctacaccggc
    10321 acgcccagcc tgggcacgct caaccgcgaa gccatggccg atttcttcag ccgctacttc
    10381 ggcaccctgc cgccggaccg tcgcaaggaa atgctggacc agttcatggc gctgcccagc
    10441 aacgacctca tcaatgtccg ttccagcacc ttccactaca aggccaatat cctgctgatc
    10501 ggcgatgcgg cgcatgccac cgccccgttc ctcgggcaag gcatgaacat ggcgctggag
    10561 gacgtccacg tattcgtgtc cctgctggaa aagcacggca atgccctggg ccctgccctg
    10621 tccgaattca cgcagcagcg caaggtgcag gcggacgcca tgcaggacat ggcgatcgcc
    10681 aactatgagg cgctgagcaa tccgaacctg attttcttcc tgcagacgcg ctacacgcgc
    10741 tacatgcaca agaaattccc ccgtgtttat ccgccagaca tggcggagaa actgtacttc
    10801 acatcggttc cttacgatga attgcagcaa atccagaaga aacaaaacgt ttggtacaaa
    10861 cttggaaggg taaattaagc tttgtggctt ttaagccagc cccgacaccc gccaacaccc
    10921 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
    10981 ccactcaa
//
