LOCUS       sequence-69073-e       12848 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12848)
  AUTHORS   Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S
  TITLE     A modular cloning system for standardized assembly of multigene 
            constructs.
  JOURNAL   PLoS One. 2011 Feb 18;6(2):e16765. doi: 
            10.1371/journal.pone.0016765.
  PUBMED    21364738
REFERENCE   2  (bases 1 to 12848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011-02-18"; volume: "6(2):e16765. doi"; pages: " 
            10.1371/journal.pone.0016765"
COMMENT     Sequence Label: pICH82113
FEATURES             Location/Qualifiers
     source          1..12848
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    26..50
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        4751..4855
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4856..5650
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      6465..6684
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             6775..7530
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     misc_feature    7533..7783
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             8109..9284
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             9284..10255
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     misc_feature    10328..10801
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     misc_feature    11151..11175
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      11433..12021
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11922..11941
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12175..12192
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12309..12330
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12345..12350,12351..12368,12369..12375)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12383..12399
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12388..12410
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12407..12423
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12407..12423
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             12419..12742
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    12436..12492
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(12493..12510)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12493..12509)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12502..12524)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(12718..12737)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gtgatccgtt taaactatca gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa
       61 agagcgttta ttagaataat cggatattta aaagggcgtg aaaaggttta tccgttcgtc
      121 catttgtatg tgccagccgc aagggtgact tcgcctatat tggacgacgg cgcgcagagg
      181 gcgacctctt tttgggttac gattgtagga ttatcactaa aacaatacat gaacatattc
      241 aaatggcaat ctctctaagg cattggaaat aaatacaaat aacagttggg tggagttttt
      301 cgacctgagg gcgttaacct tctgttaacc taaaagctct tgcccaaaca gcagaatcgg
      361 cgctaattgc cagcggcgga acttttccag tttcgcgaaa aatatcgcca ctggcaagga
      421 atgggtttga gatggcgaag tctgtcctaa aagcagcgcc tgtagttgta gggttgacgg
      481 ccttgatgga gcgtcatgcc gatgccctct cgagccaact tcaagcacat catcttaagg
      541 ttttcccgcc gcattccgag aagggcattc gaacattcgg gccatcggag gcgtccaagc
      601 tgctcggcgt tggcgagtca tatttacggc agaccgcgtc tgagatgcca gagttgaatg
      661 ttagcatgag cccgggtggc aggcgaatgt tctcaattga agatatccat gtgattcgga
      721 agtatatgga tcaggtcggc cgcgggaacc ggcgctacct gccacatcgt cgaggcggcg
      781 agcagcttca ggttatctct gtgatgaatt tcaaaggtgg gtcgggtaag accaccaccg
      841 ccgcgcatct ggcgcagtac ctcgctatgc gcggatatcg agtcttggcc attgatctcg
      901 atcctcaagc gagcctttct gcactctttg ggagccaacc ggaaacggac gttggcccga
      961 acgaaacgct ctacggcgct ataaggtatg atgatgagca ggtggcaatc gaacgagtcg
     1021 tccgagggac ttacattccc gacctccacc tgattcctgg taaccttgag ctgatggagt
     1081 ttgaacacga tacgccacgc gcgctgatga accgcaaaga gggcgacacg ctcttttatg
     1141 gtcgcatcag ccaagtaatt gaagatatcg cggataacta tgacgtcgtg gtcatcgact
     1201 gccctcccca gcttgggtat ctcacgctat ccgcattgac tgcggcgacg tccattcttg
     1261 tcacggtcca tccgcagatg ctggatgtga tgtcgatgaa ccagtttctg gcaatgacat
     1321 cgaacctttt gcgtgaaatc gagaatgctg gcgccaagtt caagtttaat tggatgcgct
     1381 atctgataac ccgtttcgaa ccgagcgacg gaccacagaa ccaaatggta ggttatctgc
     1441 ggtcgatttt tggcgaaaat gtcctcaatt ttccgatgct taaaaccacc gcggtttcgg
     1501 acgctggcct gacaaaccag actctattcg aagtggagcg tggcctgttc acgcgctcga
     1561 cctatgatcg agccttggag gcgatgaacg ccgtcaacga cgagatcgaa acactgatca
     1621 aaaaagcatg gggtaggccc acatgagccg gaagcacatc cttggcgtgt caactgacgc
     1681 ccctgaaacg tcgcccgccg acaataggac ggcaaagaac cgctccatgc cgctcctcgg
     1741 cgtaacaagg aaggagcgcg atccggcaac gaagctcaca gcgaacattg gtaacgcact
     1801 gcgagagcaa aacgatcgtc ttagccgtgc cgaagagatc gagcggcgac tcgctgaagg
     1861 tcaggcagtg atagagttgg atgcctcgtc aatagaaccg tctttcgtgc aggatcgtat
     1921 gcgaggggac attgacgggc tccttacttc gatccgggaa caaggacagc aagtcccaat
     1981 ccttgtgcga ccgcatccga gccagccggg ccgatatcag gttgccttcg gccaccgccg
     2041 gctacgcgcc gtttcagaac tcggacttcc ggtcagagcg gtcgttcgcg aactgacgga
     2101 cgagcaagtg gtcgtagcac agggtcagga aaacaatgag cgcgaagatc ttaccttcat
     2161 cgaaaaggcg cgcttcgcac atcgcctgaa caggcagttt tctcgagaga ttgtcatcgc
     2221 cgcgatgtcg atcgacaaga gcaatttgtc caagatgctt ctgctcgtcg acgccctccc
     2281 ctctgaactg accgatgcta ttggtgccgc tcctggtgtt ggacggccga gttggcaaca
     2341 acttgccgag ctgattgaga aagtttcttc accggccgac gtggctaaat atgctatgtc
     2401 ggaggaagtt caagcgctgc catcggcaga acgattcaag gcggtgatcg ctagtctgaa
     2461 gcccagtcgg gttgcgcgtg gacttcccga ggtcatggcc accccagacg gcaccagaat
     2521 tgcacaggtg acgcagagca aggccaaact ggaaatcacg attgacagga aggcgacgcc
     2581 cgattttgcg accttcgtgc tcgatcatgt gccagcgctg tatcaagcgt accacgctga
     2641 gaaccaacgg aaacggggag agtaaaccgc aaaagaaaag agccccctca acgtcgccgt
     2701 cgcggaagcc cttctgtctc tctagcgcga acagaatcgc atttcctcga atcctcgtca
     2761 agagttttta gcgccgtttt ggtgagctga tttcctttgc ctgctgaaag gtgaaagatg
     2821 atgcagacag gaagtgtaac gacgccattc gggcggcggc caatgacgct tgcgcttgtg
     2881 cggcgccaga cggcgctggc cgatatcaaa caaggcaaga cagcggacaa gtggaaggtc
     2941 tttagagatg cgtccgcggc catggaacta cttggaatcc agtccaacag tcttgccgtc
     3001 cttgatgcgc tattgagctt tcacccggaa acggagttgc gtcaggaggc acagctgatc
     3061 gtgttcccgt cgaatgctca gcttgccctt cgggcgcatg ggatggctgg cgcgactttg
     3121 cgtaggcaca tcgccatgct cgtggagtca ggcttgatcg tccggaagga tagcgccaac
     3181 ggaaagcgtt acgctcgtaa ggatggcgct ggtcagatcg agcgcgcgtt tggcttcgat
     3241 ttgtctccgc ttctcgcgcg gtccgaggag ctagcgatga tggcacagca ggtgatggcc
     3301 gatcgagcag cattcaggat ggccaaagaa agtctgacga tttgccgacg ggacgttcgg
     3361 aagctaatta cggcagctat ggaagaggga gcggagggcg actggcaagc tgtcgaggaa
     3421 gtctatgtgg aacttgtggg tagaattcca cgcgccccga cgcttgctga tgtagagtca
     3481 attctcgaag agatgtggat gctccaggaa gagataatca accggttgga aattagagac
     3541 aattcagaaa ataatagcac caatgctgcc cagagcgagc agcacataca gaattcaaaa
     3601 cccgaatccg ttaatgaact tgaacctcgc tctgaaaagg agcagggcgc taagccgagt
     3661 gaaatagacc gggcaaggag cgagccgata aaagcgttcc ccctcgggat gatcctgaaa
     3721 gcatgcccga ccattggcaa ttatgggccg agcggtgcgg ttgctagctg gcgtgacctc
     3781 atgtcggctg cggtggtggt tcggtctatg ctgggggtca gcccgtcggc ttaccaagac
     3841 gcgtgtgagg caatgggacc ggagaatgcg gcagcagcga tggcgtgcat tttggagcga
     3901 gcgaacttca tcaattcgcc cgggggctat ctccgagatc tgacacggcg gagcgagctc
     3961 gggaagtttt cacttggccc gatgataatg gcgctcttga aggctagcgg gcaggggacg
     4021 ttgcggtttg gctagaatta gcgagtatgg agcaggatgg tctgtggtca gctgaccaca
     4081 gacctaatag gttgaaaaca tgagcgtttt ttggatgatc gacagaccat ccgattcccg
     4141 gagtaccaag cgtgctctga tgggagcgat aacattactc aacaagcacg aaggccccat
     4201 gccgatcgtt gatcgtgaag gagagcctgc tctacatgcg gcggtatttt gccggccgag
     4261 gcatgtagtc gcggagcact gcctatttac tgccctaggc acaaacgttg actcttggat
     4321 cgagctggca gacaaagcaa taacccacac agaggacgat taatggctga cgaagagatc
     4381 cagaatccgc cggacggtac tgctgctgcc gaagttgagc cggctgctcc tagaggtaga
     4441 agagaaaaga aagcaccagc cgaaacagcc cgcacgggat cgttcaaatc cgtgaagccg
     4501 aaaacccgcg gcctcagcaa ccgagaaaaa ctggagaaga tcggtcaaat cgaagctcag
     4561 gtcgctggcg gcgcaacctt gaaggacgcc gttaagatcg tgggtatttc cgttcagacc
     4621 tattatcaat ggaagagagc tgcggttcaa cctgtctcac agaatccggc cgtgtctgtt
     4681 tcagttgacg atgaactcgg cgagttcatc caactcgagg aggaaaatcg gcggctcaga
     4741 aagccgagtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     4801 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatggc
     4861 taaaatgaga atatcaccgg aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga
     4921 tacggaagga atgtctcctg ctaaggtata taagctggtg ggagaaaatg aaaacctata
     4981 tttaaaaatg acggacagcc ggtataaagg gaccacctat gatgtggaac gggaaaagga
     5041 catgatgcta tggctggaag gaaagctgcc tgttccaaag gtcctgcact ttgaacggca
     5101 tgatggctgg agcaatctgc tcatgagtga ggccgatggc gtcctttgct cggaagagta
     5161 tgaagatgaa caaagccctg aaaagattat cgagctgtat gcggagtgca tcaggctctt
     5221 tcactccatc gacatatcgg attgtcccta tacgaatagc ttagacagcc gcttagccga
     5281 attggattac ttactgaata acgatctggc cgatgtggat tgcgaaaact gggaagagga
     5341 cactccattt aaagatccgc gcgagctgta tgatttttta aagacggaaa agcccgaaga
     5401 ggaacttgtc ttttcccacg gcgacctggg agacagcaac atctttgtga aagatggcaa
     5461 agtaagtggc tttattgatc ttgggagaag cggcagggcg gacaagtggt atgacattgc
     5521 cttctgcgtc cggtcgctca gggaggatat cggggaagaa cagtatgtcg agctattttt
     5581 tgacttactg gggatcaagc ctgattggga gaaaataaaa tattatattt tactggatga
     5641 attgttttag ctgttggagc ggcgtaaccg tcgcacagga aggacagaga aagcgcggat
     5701 ctgggaagtg acggacagaa cggtcaggac ctggattggg gaggcggttg ccgccgctgc
     5761 tgctgacggt gtgacgttct ctgttccggt cacaccacat acgttccgcc attcctatgc
     5821 gatgcacatg ctgtatgccg gtataccgct gaaagttctg caaagcctga tgggacataa
     5881 gtccatcagt tcaacggaag tctacacgaa ggtttttgcg ctggatgtgg ctgcccggct
     5941 ccgggtgcag tttgcgatgc cggagtctga tgcggttgcg atgctgaaac aattatcctg
     6001 agaataaatg ccttggcctt tatatggaaa tgtggaactg agtggatatg ctgtttttgt
     6061 ctgttaaaca gagaagctgg ctgttatcca ctgagaagcg aacgaaacag tcgggaaaat
     6121 ctcccattat cgtagagatc cgcattatta atctcaggag cctgtgtagc gtttatagga
     6181 agtagtgttc tgtcatgatg cctgcaagcg gtaacgaaaa cgatttgaat atgccttcag
     6241 gaacaataga aatcttcgtg cggtgttacg ttgaagtgga gcggattatg tcagcaatgg
     6301 acagaacaac ctaatgaaca cagaaccatg atgtggtctg tccttttaca gccagtagtg
     6361 ctcgccgcag tcgagcgaca gggcgaagcc ctcgagtgag cgaggaagca ccagggaaca
     6421 gcacttatat attctgctta cacacgatgc ctgaaaaaac ttcccttggg gttatccact
     6481 tatccacggg gatattttta taattatttt ttttatagtt tttagatctt cttttttaga
     6541 gcgccttgta ggcctttatc catgctggtt ctagagaagg tgttgtgaca aattgccctt
     6601 tcagtgtgac aaatcaccct caaatgacag tcctgtctgt gacaaattgc ccttaaccct
     6661 gtgacaaatt gccctcagaa gaagctgttt tttcacaaag ttatccctgc ttattgactc
     6721 ttttttattt agtgtgacaa tctaaaaact tgtcacactt cacatggatc tgtcatggcg
     6781 gaaacagcgg ttatcaatca caagaaacgt aaaaatagcc cgcgaatcgt ccagtcaaac
     6841 gacctcactg aggcggcata tagtctctcc cgggatcaaa aacgtatgct gtatctgttc
     6901 gttgaccaga tcagaaaatc tgatggcacc ctacaggaac atgacggtat ctgcgagatc
     6961 catgttgcta aatatgctga aatattcgga ttgacctctg cggaagccag taaggatata
     7021 cggcaggcat tgaagagttt cgcggggaag gaagtggttt tttatcgccc tgaagaggat
     7081 gccggcgatg aaaaaggcta tgaatctttt ccttggttta tcaaacgtgc gcacagtcca
     7141 tccagagggc tttacagtgt acatatcaac ccatatctca ttcccttctt tatcgggtta
     7201 cagaaccggt ttacgcagtt tcggcttagt gaaacaaaag aaatcaccaa tccgtatgcc
     7261 atgcgtttat acgaatccct gtgtcagtat cgtaagccgg atggctcagg catcgtgtct
     7321 ctgaaaatcg actggatcat agagcgttac cagctgcctc aaagttacca gcgtatgcct
     7381 gacttccgcc gccgcttcct gcaggtctgt gttaatgaga tcaacagcag aactccaatg
     7441 cgcctctcat acattgagaa aaagaaaggc cgccagacga ctcatatcgt attttccttc
     7501 cgcgatatca cttccatgac gacaggatag tctgagggtt atctgtcaca gatttgaggg
     7561 tggttcgtca catttgttct gacctactga gggtaatttg tcacagtttt gctgtttcct
     7621 tcagcctgca tggattttct catacttttt gaactgtaat ttttaaggaa gccaaatttg
     7681 agggcagttt gtcacagttg atttccttct ctttcccttc gtcatgtgac ctgatatcgg
     7741 gggttagttc gtcatcattg atgagggttg attatcacag tttattactc tgaattggct
     7801 atccgcgtgt gtacctctac ctggagtttt tcccacggtg gatatttctt cttgcgctga
     7861 gcgtaagagc tatctgacag aacagttctt ctttgcttcc tcgccagttc gctcgctatg
     7921 ctcggttaca cggctgcggc gagcgctagt gataataagt gactgaggta tgtggtgttc
     7981 ttatctcctt ttgtagtgtt gctcttattt taaacaactt tgcggttttt tgatgacttt
     8041 gcgattttgt tgttgctttg cagtaaattg caagatttaa taaaaaaacg caaagcaatg
     8101 attaaaggat gttcagaatg aaactcatgg aaacacttaa ccagtgcata aacgctggtc
     8161 atgaaatgac gaaggctatc gccattgcac agtttaatga tgacagcccg gaagcgagga
     8221 aaataacccg gcgctggaga ataggtgaag cagcggattt agttggggtt tcttctcagg
     8281 ctatcagaga tgccgagaaa gcagggcgac taccgcaccc ggatatggaa attcgaggac
     8341 gggttgagca acgtgttggt tatacaattg aacaaattaa tcatatgcgt gatgtgtttg
     8401 gtacgcgatt gcgacgtgct gaggacgtat ttccaccggt gatcggggtt gctgcccata
     8461 aaggtggcgt ttacaaaacc tcagtttctg ttcatcttgc tcaggatctg gctctgaagg
     8521 ggctacgtgt tttgctcgtg gaaggtaacg acccccaggg aacagcctca atgtatcacg
     8581 gatgggtacc agatcttcat attcatgcag aagatactct cctgcctttc tatcttgggg
     8641 aaaaggacga tgtcacttat gcaataaagc ccacttgctg gccggggctt gacattattc
     8701 cttcctgtct ggctctgcac cgtattgaaa ctgagttaat gggcaaattt gatgaaggta
     8761 aactgcccac cgatccacac ctgatgctcc gactggccat tgaaactgtt gctcatgact
     8821 atgatgtcat agttattgac agcgcgccta acctgggtat cggcacgatt aatgtcgtat
     8881 gtgctgctga tgtgctgatt gttcccacgc ctgctgagtt gtttgactac acctccgcac
     8941 tgcagttttt cgatatgctt cgtgatctgc tcaagaacgt tgatcttaaa gggttcgagc
     9001 ctgatgtacg tattttgctt accaaataca gcaatagtaa tggctctcag tccccgtgga
     9061 tggaggagca aattcgggat gcctggggaa gcatggttct aaaaaatgtt gtacgtgaaa
     9121 cggatgaagt tggtaaaggt cagatccgga tgagaactgt ttttgaacag gccattgatc
     9181 aacgcagttc aactggtgcc tggagaaatg ctctttctat ttgggaacct gtctgcaatg
     9241 aaattttcga tcgtctgatt aaaccacgct gggagattag ataatgaagc gtgcgcctgt
     9301 tattccaaaa catacgctca atactcaacc ggttgaagat acttcgttat cgacaccagc
     9361 tgccccgatg gtggattcgt taattgcgcg cgtaggagta atggctcgcg gtaatgccat
     9421 tactttgcct gtatgtggtc gggatgtgaa gtttactctt gaagtgctcc ggggtgatag
     9481 tgttgagaaa acctctcggg tatggtcagg taatgaacgt gaccaggagc tgcttactga
     9541 ggacgcactg gatgatctca tcccttcttt tctactgact ggtcaacaga caccggcgtt
     9601 cggtcgaaga gtatctggtg tcatagaaat tgccgatggg agtcgccgtc gtaaagctgc
     9661 tgcacttacc gaaagtgatt atcgtgttct ggttggcgag ctggatgatg agcagatggc
     9721 tgcattatcc agattgggta acgattatcg cccaacaagt gcttatgaac gtggtcagcg
     9781 ttatgcaagc cgattgcaga atgaatttgc tggaaatatt tctgcgctgg ctgatgcgga
     9841 aaatatttca cgtaagatta ttacccgctg tatcaacacc gccaaattgc ctaaatcagt
     9901 tgttgctctt ttttctcacc ccggtgaact atctgcccgg tcaggtgatg cacttcaaaa
     9961 agcctttaca gataaagagg aattacttaa gcagcaggca tctaaccttc atgagcagaa
    10021 aaaagctggg gtgatatttg aagctgaaga agttatcact cttttaactt ctgtgcttaa
    10081 aacgtcatct gcatcaagaa ctagtttaag ctcacgacat cagtttgctc ctggagcgac
    10141 agtattgtat aagggcgata aaatggtgct taacctggac aggtcacgtg ttccaactga
    10201 gtgtatagag aaaattgagg ccattcttaa ggaacttgaa aagccagcac cctgatgcga
    10261 ccacgtttta gtctacgttt atctgtcttt acttaatgtc ctttgttaca ggccagaaag
    10321 cataactggc ctgaatattc tctctgggcc cactgttcca cttgtatcgt cggtctgata
    10381 atcagactgg gaccacggtc ccactcgtat cgtcggtctg attattagtc tgggaccacg
    10441 gtcccactcg tatcgtcggt ctgattatta gtctgggacc acggtcccac tcgtatcgtc
    10501 ggtctgataa tcagactggg accacggtcc cactcgtatc gtcggtctga ttattagtct
    10561 gggaccatgg tcccactcgt atcgtcggtc tgattattag tctgggacca cggtcccact
    10621 cgtatcgtcg gtctgattat tagtctggaa ccacggtccc actcgtatcg tcggtctgat
    10681 tattagtctg ggaccacggt cccactcgta tcgtcggtct gattattagt ctgggaccac
    10741 gatcccactc gtgttgtcgg tctgattatc ggtctgggac cacggtccca cttgtattgt
    10801 cgatcagact atcagcgtga gactacgatt ccatcaatgc ctgtcaaggg caagtattga
    10861 catgtcgtcg taacctgtag aacggagtaa cctcggtgtg cggttgtatg cctgctgtgg
    10921 attgctgctg tgtcctgctt atccacaaca ttttgcgcac ggttatgtgg acaaaatacc
    10981 tggttaccca ggccgtgccg gcacgttaac cgggctgcat ccgatgcaag tgtgtcgctg
    11041 tcgacctgct cggatctgtt ggaccggaca gtagtcatgg ttgatgggct gcctgtatcg
    11101 agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg tggcaggata
    11161 tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg gacgttttta
    11221 atgtactggg gttgaacact ctgtgaagac aatgcctgag acctgtcaga ccaagtttac
    11281 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
    11341 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
    11401 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
    11461 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
    11521 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
    11581 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
    11641 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
    11701 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
    11761 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
    11821 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
    11881 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
    11941 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
    12001 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
    12061 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
    12121 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
    12181 tcagtgagcg aggaagcgga tgagcgccca atacgcaaac cgcctctccc cgcgcgttgg
    12241 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
    12301 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
    12361 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
    12421 gaccatgatt acgccaagct tgcatgcctg caggtcgact ctagaggatc cccgggtacc
    12481 gagctcgaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
    12541 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
    12601 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg
    12661 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac
    12721 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
    12781 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga cggtctcagg
    12841 gacacgaa
//
