LOCUS       sequence-69078-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of query pri-miRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 V5-DEST mir-1-1 reporter
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(541..846)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(643..662)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 aaaaagctga acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc
       61 ataaaaaaca gactacataa tactgtaaaa cacaacatat ccagtcacta tgaatcaact
      121 acttagatgg tattagtgac ctgtagtcga ccgacagcct tccaaatgtt cttcgggtga
      181 tgctgccaac ttagtcgacc gacagccttc caaatgttct tctcaaacgg aatcgtcgta
      241 tccagcctac tcgctattgt cctcaatgcc gtattaaatc ataaaaagaa ataagaaaaa
      301 gaggtgcgag cctctttttt gtgtgacaaa ataaaaacat ctacctattc atatacgcta
      361 gtgtcatagt cctgaaaatc atctgcatca agaacaattt cacaactctt atacttttct
      421 cttacaagtc gttcggcttc atctggattt tcagcctcta tacttactaa acgtgataaa
      481 gtttctgtaa tttctactgt atcgacctgc agactggctg tgtataaggg agcctgacat
      541 ttatattccc cagaacatca ggttaatggc gtttttgatg tcattttcgc ggtggctgag
      601 atcagccact tcttccccga taacggagac cggcacactg gccatatcgg tggtcatcat
      661 gcgccagctt tcatccccga tatgcaccac cgggtaaagt tcacgggaga ctttatctga
      721 cagcagacgt gcactggcca gggggatcac catccgtcgc ccgggcgtgt caataatatc
      781 actctgtaca tccacaaaca gacgataacg gctctctctt ttataggtgt aaaccttaaa
      841 ctgcatttca ccag
//
