LOCUS       sequence-69079-e         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of query pri-miRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 V5-DEST mir-1-1 reporter
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(3..44)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    397..521
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        699..801
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(868..887)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cgcgtagaat cgagaccgag gagagggtta gggataggct taccttcgaa tgtctggtga
       61 gcacttccac ctgctgacac aggcaaagtg acagaacaat gctggcgggg acacgaccgt
      121 ccaccaacgc ccaggcagcc tgccgcccca gcctcacccc caccacagcc gcctggctgg
      181 ctgtcggccg cggcggcccc cgtggtgtgg agtgcccctc agtgcggcga gagaggtccc
      241 ggcctgagat acatacttct ttacattcca tagcttagca ggtccatatg ggcatataaa
      301 gaagtatgtt tcccaagcag gcagtctgca tgcagttcca agaccccggg acgccgcccc
      361 ggtagaagct gaccccactg cgggccctct agatcaacca ctttgtacaa gaaagctgaa
      421 cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca taaaaaacag
      481 actacataat actgtaaaac acaacatatc cagtcactat gaatcaacta cttagatggt
      541 attagtgacc tgtagtcgac taagttggca gcatcacccg acgcactttg cgccgaataa
      601 atacctgtga cggaagatca cttcgcagaa taaataaatc ctggtgtccc tgttgatacc
      661 gggaagccct gggccaactt ttggcgaaaa tgagacgttg atcggcacgt aagaggttcc
      721 aactttcacc ataatgaaat aagatcacta ccgggcgtat tttttgagtt atcgagattt
      781 tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata
      841 tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat
      901 aaccagaccg ttcagctgg
//
