LOCUS       sequence-69080-e         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Lemaire JF, McPherson PS
  TITLE     Binding of Vac14 to neuronal nitric oxide synthase: Characterisation
            of a new internal PDZ-recognition motif.
  JOURNAL   FEBS Lett. 2006 Dec 22;580(30):6948-54. Epub 2006 Dec 4.
  PUBMED    17161399
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS 
            Lett."; date: "2006-12-22"; volume: "580(30)"; pages: "6948-54. Epub
            2006 Dec 4"
COMMENT     Sequence Label: Flag-Vac 14 Full Length
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        40..58
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      93..102
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             102..125
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgattacgc caagctcgaa attaaccctc actaaaggga
       61 acaaaagctg gagctccacc gcggtggcgg ccgccaccat ggattacaag gatgacgacg
      121 ataagagccc gggcggatct atgaaccccg agaaggattt cgcgccgctc acgcctaaca
      181 tcgtgcgcgc cctcaatgac aagctgtacg aaaagcggaa ggtggcagcg ctggagatcg
      241 agaagctggt ccgggagttc gtggcccaga acaataccgt gcaaatcaag catgtgatcc
      301 agaccctgtc ccaggagttt gccctgtctc agcaccccca cagccggaaa gggggcctca
      361 tcggcctggc cgcctgctcc atcgcactgg gcaaggactc agggctctac ctgaaggagc
      421 tgatcgagcc agtgctgacc tgcttcaatg atgcagacag caggctgcgc tactatgcct
      481 gcgaggccct ctacaacatc gtcaaggtgg cccggggcgc tgtgctgccc cacttcaacg
      541 tgctctttga cgggctgagc aagctggcag ccgacccaga ccccaatgtg aaaagcggat
      601 ctgagctcct agaccgcctt ttaaaggaca ttgtgactga gagcaacaag tttgacctgg
      661 tgagcttcat ccccttgttg cgagagagga tttactccaa caaccagtat gcccggcagt
      721 tcatcatctc ctggatcctg gttctggagt cggtgccaga cattaacctg ctggattacc
      781 tgccggagat cctggatgga ctcttccaga tcctgggtga caatggcaaa gagattcgca
      841 aaatgtgtga ggttgttctt ggagaattct taaaagaaat taa
//
