LOCUS       sequence-69082-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human KRas protein from chimeric cDNA sequence derived 
            primarily from HRAS codons with a KRAS 3' tail. Results in higher 
            than normal exogenous expression of KRas protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Lampson BL, Pershing NL, Prinz JA, Lacsina JR, Marzluff WF, 
            Nicchitta CV, MacAlpine DM, Counter CM
  TITLE     Rare codons regulate KRas oncogenesis.
  JOURNAL   Curr Biol. 2013 Jan 7;23(1):70-5. doi: 10.1016/j.cub.2012.11.031. 
            Epub 2012 Dec 13.
  PUBMED    23246410
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2012.11.031"; journalName: "Curr Biol"; date: 
            "2013-01-7- 7"; volume: "23"; issue: "1"; pages: "70-5"
COMMENT     Sequence Label: pBABE-Puro-KRas*
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..27
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(603..623)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 atggactaca aggacgacga tgacaagacg gaatataagc ttgtggtggt gggcgctgga
       61 ggcgtgggaa agagtgccct gaccatccag ctgatccaga accactttgt ggacgaatac
      121 gaccccacta tagaggattc ctaccggaag caggtggtca ttgatgggga gacgtgcctg
      181 ttggacatcc tggataccgc cggccaggag gagtacagcg ccatgcggga ccagtacatg
      241 cgcaccgggg agggcttcct gtgtgtgttt gccatcaaca acaccaagtc ttttgaggac
      301 atccaccatt acagggagca gatcaaacgg gtgaaggact cggaggacgt gcccatggtg
      361 ctggtgggga acaagtgtga cctgccttca cgcactgtgg acactaagca ggctcaggac
      421 ctcgcccgaa gctacggcat ccccttcatc gagacctcgg ccaagacccg gcagggagtg
      481 gatgatgcct tctatacact agttcgagaa attcgaaaac ataaagaaaa gatgagcaaa
      541 gacggtaaaa agaagaaaaa gaagtcaaag acaaagtgtg taattatgta agtcgaccct
      601 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
      661 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
      721 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
      781 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat t
//
