LOCUS       sequence-69083-e         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Katoh Y, Ritter B, Gaffry T, Blondeau F, Honing S, McPherson PS
  TITLE     The clavesin family, neuron-specific lipid- and clathrin-binding 
            Sec14 proteins regulating lysosomal morphology.
  JOURNAL   J Biol Chem. 2009 Oct 2;284(40):27646-54. doi: 
            10.1074/jbc.M109.034884. Epub 2009 Aug 3.
  PUBMED    19651769
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M109.034884"; journalName: "J Biol Chem"; date: 
            "2009-10-2- 2"; volume: "284"; issue: "40"; pages: "27646-54"
COMMENT     Sequence Label: Flag-Tagged Clavesin2 (pCMV-Tag2B-Sec14b)
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        36..54
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      89..98
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             98..121
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 accgtcagat ccgctagcga ttacgccaag ctcgaaatta accctcacta aagggaacaa
       61 aagctggagc tccaccgcgg tggcggccgc caccatggat tacaaggatg acgacgataa
      121 gagcccgggc ggatccatga ctcatttgca agccggtctc tcccctgaga ccctggagaa
      181 agctcgcctg gagctcaatg aaaacccaga cacgctgcac caggacatcc aggaggtgag
      241 ggatatggtc atcaccaggc cggacattgg ctttctgcgc acggatgatg ccttcatctt
      301 acgcttcttg cgggctagga agtttcatca ctttgaggcc ttccgcctcc tggcgcagta
      361 ctttgagtac cggcagcaga acctggacat gttcaaaagc tttaaggcca ccgaccctgg
      421 catcaagcag gcactgaagg atggcttccc tgggggcctg gccaatctgg accactatgg
      481 caggaagatt ctagtccttt ttgctgccaa ttgggatcag agcaggtaca cactggtgga
      541 tattttgcgt gccatcttac tttctttaga agccatgatt gaagatcctg agcttcaagt
      601 gaatgggttt gttttgatca tagactggag taacttcact ttcaagcaag cctctaaact
      661 cacaccaagt atgctgcgat tagctattga aggcctgcag gatagtttcc cagcgcgatt
      721 tggaggaatt cattttgtca atcaaccatg gtatatccat gccctgtaca ccgtgatccg
      781 gcctttcctg aaggagaaaa ctcggaaaag gatatttttg catggtaaca acctgaacag
      841 tctacaccaa ctaattcatc ctgagatcct gccctctgag tttggaggaa tgctgcctcc
      901 ttatgacatg gggacatggg caagaacact gctagaccat gaata
//
