LOCUS       sequence-69084-e         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human oncogenic KRas protein from chimeric cDNA sequence 
            derived primarily from HRAS codons with a KRAS 3' tail. Results in 
            higher than normal exogenous expression of KRas protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Lampson BL, Pershing NL, Prinz JA, Lacsina JR, Marzluff WF, 
            Nicchitta CV, MacAlpine DM, Counter CM
  TITLE     Rare codons regulate KRas oncogenesis.
  JOURNAL   Curr Biol. 2013 Jan 7;23(1):70-5. doi: 10.1016/j.cub.2012.11.031. 
            Epub 2012 Dec 13.
  PUBMED    23246410
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2012.11.031"; journalName: "Curr Biol"; date: 
            "2013-01-7- 7"; volume: "23"; issue: "1"; pages: "70-5"
COMMENT     Sequence Label: pBABE-Puro-KRas*G12V
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             5..28
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(604..624)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 catggactac aaggacgacg atgacaagac ggaatataag cttgtggtgg tgggcgctgt
       61 aggcgtggga aagagtgccc tgaccatcca gctgatccag aaccactttg tggacgaata
      121 cgaccccact atagaggatt cctaccggaa gcaggtggtc attgatgggg agacgtgcct
      181 gttggacatc ctggataccg ccggccagga ggagtacagc gccatgcggg accagtacat
      241 gcgcaccggg gagggcttcc tgtgtgtgtt tgccatcaac aacaccaagt cttttgagga
      301 catccaccat tacagggagc agatcaaacg ggtgaaggac tcggaggacg tgcccatggt
      361 gctggtgggg aacaagtgtg acctgccttc acgcactgtg gacactaagc aggctcagga
      421 cctcgcccga agctacggca tccccttcat cgagacctcg gccaagaccc ggcagggagt
      481 ggatgatgcc ttctatacac tagttcgaga aattcgaaaa cataaagaaa agatgagcaa
      541 agacggtaaa aagaagaaaa agaagtcaaa gacaaagtgt gtaattatgt aagtcgaccc
      601 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
      661 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
      721 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
      781 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
      841 taatttttt
//
