LOCUS       sequence-69086-e         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR
            array designed for the easy cloning of new spacers..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Bikard D, Jiang W, Samai P, Hochschild A, Zhang F, Marraffini LA
  TITLE     Programmable repression and activation of bacterial gene expression 
            using an engineered CRISPR-Cas system.
  JOURNAL   Nucleic Acids Res. 2013 Jun 12.
  PUBMED    23761437
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Jun 12."
COMMENT     Sequence Label: pdCas9
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tgctgttttg aatggttcca acaagattat tttataactt ttataacaaa taatcaagga
       61 gaaattcaaa gaaatttatc agccataaaa caatacttaa tactatagaa tgataacaaa
      121 ataaactact ttttaaaaga attttgtgtt ataatctatt tattattaag tattgggtaa
      181 tattttttga agagatattt tgaaaaagaa aaattaaagc atattaaact aatttcggag
      241 gtcattaaaa ctattattga aatcatcaaa ctcattatgg atttaattta aactttttat
      301 tttaggaggc aaaaatggat aagaaatact caataggctt agctatcggc acaaatagcg
      361 tcggatgggc ggtgatcact gatgaatata aggttccgtc taaaaagttc aaggttctgg
      421 gaaatacaga ccgccacagt atcaaaaaaa atcttatagg ggctctttta tttgacagtg
      481 gagagacagc ggaagcgact cgtctcaaac ggacagctcg tagaaggtat acacgtcgga
      541 agaatcgtat ttgttatcta caggagattt tttcaaatga gatggcgaaa gtagatgata
      601 gtttctttca tcgacttgaa gagtcttttt tggtggaaga agacaagaag catgaacgtc
      661 atcctatttt tggaaatata gtagatgaag ttgcttatca tgagaaatat ccaactatct
      721 atcatctgcg aaaaaaattg gtagattcta ctgataaagc ggatttgcgc ttaatctatt
      781 tggccttagc gcatatgatt aagtttcgtg gtcatttttt gattgaggga gatt
//
