LOCUS       sequence-69089-e         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Same as pdCas9, but with the w-dCas9 fusion (w is fused at the 
            N-terminal end of dCas9).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Bikard D, Jiang W, Samai P, Hochschild A, Zhang F, Marraffini LA
  TITLE     Programmable repression and activation of bacterial gene expression 
            using an engineered CRISPR-Cas system.
  JOURNAL   Nucleic Acids Res. 2013 Jun 12.
  PUBMED    23761437
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Jun 12."
COMMENT     Sequence Label: pWJ68
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             335..607
                     /codon_start=1
                     /gene="E. coli rpoZ"
                     /product="DNA-directed RNA polymerase subunit omega"
                     /label=RNAP omega
                     /note="promotes RNA polymerase assembly"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEENDK
                     TTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
ORIGIN
        1 tgctgttttg aatggttcca acaagattat tttataactt ttataacaat taatcaagga
       61 gaaattcaaa gaaatttatc agccataaaa caatacttaa tactatagaa tgataacaaa
      121 ataaactact ttttaaaaga attttgtgtt ataatctatt tattattaag tattgggtaa
      181 tattttttga agagatattt tggaaaagaa aaattaaagc atattaaact aatttcggag
      241 gtcattaaaa ctattattga acaaattgtt ggcagactga acctgatttc agtatcatgc
      301 ccagtcattt cttcacctgt ggagcttttt aagtatggca cgcgtaactg ttcaggacgc
      361 tgtagagaaa attggtaacc gttttgacct ggtactggtc gccgcgcgtc gcgctcgtca
      421 gatgcaggta ggcggaaagg atccgctggt accggaagaa aacgataaaa ccactgtaat
      481 cgcgctgcgc gaaatcgaag aaggtctgat caacaaccag atcctcgacg ttcgcgaacg
      541 ccaggaacag caagagcagg aagccgctga attacaagcc gttaccgcta ttgctgaagg
      601 tcgtcgtgct gcagataaga aatactcaat aggcttagct atcggcacaa atagcgtcgg
      661 atgggcggtg atcactgatg aatataaggt tccgtctaaa aagttcaagg ttctgggaaa
      721 tacagaccgc cacagtatca aaaaaaatct tataggggct cttttatttg acagtggaga
      781 gacagcggaa gcgactcgtc tcaaacggac agctcgtaga aggtatacac gtcggaa
//
