LOCUS       sequence-69090-e         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR
            array designed for the easy cloning of new spacers..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Bikard D, Jiang W, Samai P, Hochschild A, Zhang F, Marraffini LA
  TITLE     Programmable repression and activation of bacterial gene expression 
            using an engineered CRISPR-Cas system.
  JOURNAL   Nucleic Acids Res. 2013 Jun 12.
  PUBMED    23761437
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Jun 12."
COMMENT     Sequence Label: pdCas9
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     repeat_region   397..432
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   463..498
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     misc_feature    complement(499..630)
                     /label=crRNA leader
                     /note="crRNA leader sequence for the Streptococcus pyogenes
                     CRISPR/Cas system"
ORIGIN
        1 ccggccacga tgcgtccggc gtagaggatc cacaggacgg gtgtggtcgc catgatcgcg
       61 tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg
      121 gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc
      181 acgccatagt gactggcgat gctgtcggaa tggacgatca cactactctt cttttgccta
      241 ttataacatg aaaacgcttt aacagaaaga ataggaaggt atccgactgc tggtattaac
      301 cctctttctc aagttatcat cggcaatgtt gaatggagtc cattcaaaac agcatagctc
      361 taaaacatgg aactcaacaa gtctcagtgt gctgaagttt tgggaccatt caaaacagca
      421 tagctctaaa acgagacctt tgagcttccg agactggtct cagttttggg accattcaaa
      481 acagcatagc tctaaaacct cgtagactat ttttgtctaa aaaatttcgt aatcgcacta
      541 tttgtctcag ctagacttca gtcttgaaaa gcccctgtat tactgcattt attaagagta
      601 ttataccata tttttagtta ttaagaaata atcttcatct aaaatatact tcagtcacct
      661 cctagctgac tcaaatcaat gcgtgtttca taaagaccag tgatggattg atggataaga
      721 gtggcatcta aaacttcttt tgtagacgta tatcgtttac gatcaattgt tgtatcaaaa
      781 tatttaaaag cagcgggagc tccaagattc gtcaacgtaa ataaatgaat aatat
//
