LOCUS       sequence-69093-e         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Same as pdCas9, but with the dCas9-w fusion (w is fused at the 
            C-terminal end of dCas9).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Bikard D, Jiang W, Samai P, Hochschild A, Zhang F, Marraffini LA
  TITLE     Programmable repression and activation of bacterial gene expression 
            using an engineered CRISPR-Cas system.
  JOURNAL   Nucleic Acids Res. 2013 Jun 12.
  PUBMED    23761437
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Jun 12."
COMMENT     Sequence Label: pWJ66
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     repeat_region   397..432
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   463..498
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     misc_feature    complement(499..600)
                     /label=crRNA leader
                     /note="crRNA leader sequence for the Streptococcus pyogenes
                     CRISPR/Cas system"
ORIGIN
        1 ccggccacga tgcgtccggc gtagaggatc cacaggacgg gtgtggtcgc catgatcgcg
       61 tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg
      121 gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc
      181 acgccatagt gactggcgat gctgtcggaa tggacgatca cactactctt cttttgccta
      241 ttataacatg aaaacgcttt aacagaaaga ataggaaggt atccgactgc tggtattaac
      301 cctctttctc aagttatcat cggcaatgtt gaatggagac cattcaaaac agcatagctc
      361 taaaacatgg aactcaacaa gtctcagtgt gctgaagttt tgggaccatt caaaacagca
      421 tagctctaaa acgagacctt tgagcttccg agactggtct cagttttggg accattcaaa
      481 acagcatagc tctaaaacct cgtagactat ttttgtctaa aaaatttcgt aatcgcacta
      541 tttgtctcag ctagacttca gtcttgaaaa gcccctgtat tactgcattt attaagagta
      601 ttaacgacga ccttcagcaa tagcggtaac ggcttgtaat tcagcggctt cctgctcttg
      661 ctgttcctgg cgttcgcgaa cgtcgaggat ctggttgttg atcagacctt cttcgatttc
      721 gcgcagcgcg attacagtgg ttttatcgtt ttcttccggt accagcggat cctttccgcc
      781 tacctgcatc tgacgagcgc gacgcgcggc gaccagtacc aggtcaaaac g
//
