LOCUS       sequence-69101-e         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Same as pdCas9, but with the dCas9-w fusion (w is fused at the 
            C-terminal end of dCas9).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Bikard D, Jiang W, Samai P, Hochschild A, Zhang F, Marraffini LA
  TITLE     Programmable repression and activation of bacterial gene expression 
            using an engineered CRISPR-Cas system.
  JOURNAL   Nucleic Acids Res. 2013 Jun 12.
  PUBMED    23761437
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Jun 12."
COMMENT     Sequence Label: pWJ66
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(84..89,90..106,107..112)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     misc_RNA        complement(430..508)
                     /label=tracrRNA
                     /note="trans-activating CRISPR RNA for the Streptococcus 
                     pyogenes CRISPR/Cas9 system"
ORIGIN
        1 atttctagat ttcagtgcaa tttatctctt caaatgtagc acctgaagtc agccccatac
       61 gatataagtt gtaattctaa tgtttgacag cttatcatcg ataagcttta atgcggtagt
      121 ttatcacagt taaattgcta acgcagtcag gcaccgtgta tgaaatctaa caatgcgctc
      181 atcgtcatcc tcggcaccgt caccctggat gctgtaggca taggcttggt tatgccggta
      241 ctgccgggcc tcttgcggga ttacgaaatc atcctgtgga gcttagtagg tttagcaaga
      301 tggcagcgcc taaatgtaga atgataaaag gattaagaga ttaatttccc taaaaatgat
      361 aaaacaagcg ttttgaaagc gcttgttttt ttggtttgca gtcagagtag aatagaagta
      421 tcaaaaaaag caccgactcg gtgccacttt ttcaagttga taacggacta gccttatttt
      481 aacttgctat gctgttttga atggttccaa caagattatt ttataacttt tataacaaat
      541 aatcaaggag aaattcaaag aaatttatca gccataaaac aatacttaat actatagaat
      601 gataacaaaa taaactactt tttaaaagaa ttttgtgtta taatctattt attattaagt
      661 attgggtaat attttttgaa gagatatttt gaaaaagaaa aattaaagca tattaaacta
      721 atttcggagg tcattaaaac tattattgaa atcatcaaac tcattatgga ttta
//
