LOCUS       sequence-69111-e         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Same as pdCas9, but with the w-dCas9 fusion (w is fused at the 
            N-terminal end of dCas9).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Bikard D, Jiang W, Samai P, Hochschild A, Zhang F, Marraffini LA
  TITLE     Programmable repression and activation of bacterial gene expression 
            using an engineered CRISPR-Cas system.
  JOURNAL   Nucleic Acids Res. 2013 Jun 12.
  PUBMED    23761437
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Jun 12."
COMMENT     Sequence Label: pWJ68
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     repeat_region   388..423
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   454..489
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     misc_feature    complement(490..621)
                     /label=crRNA leader
                     /note="crRNA leader sequence for the Streptococcus pyogenes
                     CRISPR/Cas system"
ORIGIN
        1 atgcgtccgg cgtagaggat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata
       61 gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct
      121 ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag
      181 tgactggcga tgctgtcgga atggacgatc acactactct tcttttgcct attataacat
      241 gaaaacgctt taacagaaag aataggaagg tatccgactg ctggtattaa ccctctttct
      301 caagttatca tcggcaatgt tgaatggagt ccattcaaaa cagcatagct ctaaaacatg
      361 gaactcaaca agtctcagtg tgctgaagtt ttgggaccat tcaaaacagc atagctctaa
      421 aacgagacct ttgagcttcc gagactggtc tcagttttgg gaccattcaa aacagcatag
      481 ctctaaaacc tcgtggacta tttttgtcta aaaaatttcg taatcgcact atttgtctca
      541 gctagacttc agtcttgaaa agcccctgta ttactgcatt tattaagagt attataccat
      601 atttttagtt attaagaaat aatcttcatc taaaatatac ttcagtcacc tcctagctga
      661 ctcaaatcaa tgcgtgtttc ataaagacca gtgatggatt gatggataag agtggcatct
      721 aaaacttctt ttgtagacgt gtatcgttta cgatcaattg ttgtatcaaa atatttaaaa
      781 gcagcgggag ctccaagatt cgtcaacgta aataaatgaa taatattttc tgcttg
//
