LOCUS       sequence-69114-e         754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Same as pdCas9, but with the w-dCas9 fusion (w is fused at the 
            N-terminal end of dCas9).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Bikard D, Jiang W, Samai P, Hochschild A, Zhang F, Marraffini LA
  TITLE     Programmable repression and activation of bacterial gene expression 
            using an engineered CRISPR-Cas system.
  JOURNAL   Nucleic Acids Res. 2013 Jun 12.
  PUBMED    23761437
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Jun 12."
COMMENT     Sequence Label: pWJ68
FEATURES             Location/Qualifiers
     source          1..754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(86..91,92..108,109..114)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     misc_RNA        complement(432..510)
                     /label=tracrRNA
                     /note="trans-activating CRISPR RNA for the Streptococcus 
                     pyogenes CRISPR/Cas9 system"
ORIGIN
        1 atatttctag atttcagtgc aatttatctc ttcaaatgta gcacctgaag tcagccccat
       61 acgatataag ttgtaattct aatgtttgac agcttatcat cgataagctt taatgcggta
      121 gtttgtcaca gttaaattgc taacgcagtc aggcaccgtg tatgaaatct aacaatgcgc
      181 tcatcgtcat cctcggcacc gtcaccctgg atgctgtagg cataggcttg gttatgccgg
      241 tactgccggg cctcttgcgg gattacgaaa tcatcctgtg gagcttagta ggtttagcaa
      301 gatggcagcg cctaaatgta gaatgataaa aggattaaga gattaatttc cctaaaaatg
      361 ataaaacaag cgttttgaaa gcgcttgttt ttttggtttg cagtcagagt agaatagaag
      421 tatcaaaaaa agcaccgact cggcgccact ttttcaagtt gataacggac tagccttatt
      481 ttaacttgct atgctgtttt gaatggttcc aacaagatta ttttataact tttataacaa
      541 ttaatcaagg agaaattcaa agaaatttat cagccataaa acaatactta atactataga
      601 atgataacaa aataaactac tttttaaaag aattttgtgt tataatctat ttattattaa
      661 gtattgggta atattttttg aagagatatt ttggaaaaga aaaattaaag catattaaac
      721 taatttcgga ggtcattaaa actattattg aaca
//
