LOCUS       sequence-69157-e         704 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid containing the targeting construct to insert a GFP-FLAG tag 
            at the N-terminus of the human XRCC6 gene coding for Ku70..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Britton S, Coates J, Jackson SP
  TITLE     A new method for high-resolution imaging of Ku foci to decipher 
            mechanisms of DNA double-strand break repair.
  JOURNAL   J Cell Biol. 2013 Jul 29.
  PUBMED    23897892
REFERENCE   2  (bases 1 to 704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2013 Jul 29."
COMMENT     Sequence Label: pAAV-Ku70-TC
FEATURES             Location/Qualifiers
     source          1..704
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             30..53
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 acgagctgta caagtccgga ctcagatctg attacaagga tgacgacgat aagaccggtt
       61 cagggtggga gtcatattac aaaaccgagg gcgatgaaga agcagaggaa gaacaagaag
      121 agaaccttga agcaagtggt aagtgacttc agcatgtagt gccattcggt gtgtggaaga
      181 aagaccttcc ctgcctatct ctgctggatg gactttgctg tttgatggcc tgcccttctc
      241 ctcccagata attctgaaat tcttttcttc ctttgaactt cgcagagcta ccctaggctc
      301 ttatatgcag atcttactct agtgtgccct aagttttttt tggacactgg tagtttgaca
      361 tcttttggtc cctgataaat ggccatgacc ataggtattt tttgtcatct taagtggttc
      421 aggtgagacc cttctcatcc aggaaagtag tgttaccatt ccaggacctc agtgtaaaaa
      481 tgcccccacc ctgcaataac tacaccaacc aaagaataaa cgcaagcaac acagttacag
      541 gaaagtgatt gattccctca ctatagcctc cacgtttggg agtgtatgtg caggcaacac
      601 agtaacacat aaaacaaagg aagagaaata ggggtacatg taatcttcat actggttaat
      661 ttaactcctg agcagaaaaa ttttgaatat ttttattaaa aatg
//
