LOCUS       sequence-69160-e         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for constitutive or doxycycline-inducible expression of 
            wild-type human Ku70 resistant to a siRNA. Confers resistance to 
            puromycin. Use T-REx cells for doxycycline-inducible expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Britton S, Coates J, Jackson SP
  TITLE     A new method for high-resolution imaging of Ku foci to decipher 
            mechanisms of DNA double-strand break repair.
  JOURNAL   J Cell Biol. 2013 Jul 29.
  PUBMED    23897892
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2013 Jul 29."
COMMENT     Sequence Label: pICE-EGFP-FLAG-Ku70siR-WT
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             30..53
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 acgagctgta caagtccgga ctcagatctg attacaagga tgacgacgat aagctcgagt
       61 cagggtggga gtcatattac aaaaccgagg gcgatgaaga agcagaggaa gaacaagaag
      121 agaaccttga agcaagtgga gactataaat attcaggaag agatagtttg atttttttgg
      181 ttgatgcctc caaggctatg tttgaatctc aatccgagga cgaactcacc ccttttgaca
      241 tgagcatcca gtgtatccaa agtgtgtaca tcagtaagat cataagcagt gatcgagatc
      301 tcttggctgt ggtgttctat ggtaccgaga aagacaaaaa ttcagtgaat tttaaaaata
      361 tttacgtctt acaggagctg gataatccag gtgcaaaacg aattctagag cttgaccagt
      421 ttaaggggca gcagggacaa aaacgtttcc aagacatgat gggccacgga tctgactact
      481 cactcagtga agtgctgtgg gtctgtgcca acctctttag tgatgtccaa ttcaagatga
      541 gtcataagag gatcatgctg ttcaccaatg aagacaaccc ccatggcaat gacagtgcca
      601 aagccagccg ggccaggacc aaagccggtg atctccgaga tacaggcatc ttccttgact
      661 tgatgcacct gaagaaacct gggggctttg acatatcctt gttctacaga gatatcatca
      721 gcatagcaga ggatgaggac ctcagggttc actttgagga atccagcaag ctagaagacc
      781 tgttgcggaa ggttcgcgcc aaggagacaa ggaagcgagc actcagcagg ttaa
//
