LOCUS       sequence-69161-e         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For constitutive or doxycycline-inducible expression of the site 
            specific homing nuclease I-PpoI (human codon optimized). Confers 
            resistance to puro. Use T-REx cells for doxy-inducible expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Britton S, Coates J, Jackson SP
  TITLE     A new method for high-resolution imaging of Ku foci to decipher 
            mechanisms of DNA double-strand break repair.
  JOURNAL   J Cell Biol. 2013 Jul 29.
  PUBMED    23897892
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2013 Jul 29."
COMMENT     Sequence Label: pICE-HA-NLS-I-PpoI
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(510..530)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(534..560)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(646..670)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    680..698
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    701..719
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(721..772)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(750..770)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gtctcgagtt acaccacgaa gtgggagccc ctctgctgag ggccagccac tgtagcgcca
       61 gggccataca gaggtccctg tctcaggcac acgacggcat gcacacatcc gccattaggg
      121 ccagggcacc aattccggcc cttgttgtcg tccaggctct cccagcacag gtgcaggggg
      181 ttgtggcatc tggtgttgtg gcacaggtgg ctggcggtgc aggtcttgcc gttgatgttg
      241 tcgggttcca gcagaaaggg cacggtgtgg ctgccccatc tgtgcaccac ctggttgatg
      301 gtccgcttgt actgccatct ggtggggccg ttcttggcaa agcccacgcc gtaaggagcg
      361 gccaggggga tctcgtaaca gtgcagggtt ccctggaggc ctccgcccag aggcacatgg
      421 tgggtgatca cggggaactg gcccacggtt tcctcccagc tgtcgatcac ggccaggatc
      481 tgggcgttgg tcagggccat ggatccgccc actttccgct ttttcttggg tccggcgtaa
      541 tcgggcacgt cgtaggggta catggtggcg gcaagcttgc cggaggctgg atcggtcccg
      601 gtgtcttcta tggaggtcaa aacagcgtgg atggcgtctc caggcgatct gacggttcac
      661 taaacgagct cgtcgacgat ctctatcact gatagggaga tctctatcac tgatagggag
      721 agctctgctt atatagacct cccaccgtac acgcctaccg cccatttgcg tcaatggggc
      781 ggagttgtta cgacattttg gaaagtcccg ttg
//
