LOCUS       sequence-69166-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for cloning and constitutive or doxycycline-inducible 
            expression of cDNAs. Confers resistance to puromycin. Use T-REx 
            cells for doxycycline-inducible expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Britton S, Coates J, Jackson SP
  TITLE     A new method for high-resolution imaging of Ku foci to decipher 
            mechanisms of DNA double-strand break repair.
  JOURNAL   J Cell Biol. 2013 Jul 29.
  PUBMED    23897892
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2013 Jul 29."
COMMENT     Sequence Label: pICE
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      84..93
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(167..191)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    201..219
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    222..240
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(242..445)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(271..291)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        446..825
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 cagacgcgtc tcgagggtac cggatccgaa ttcgcggccg catttgtctg tgatgccggc
       61 ggcgttcacg tactcgtgca gcaccatggt ggcaagcttg ccggaggctg gatcggtccc
      121 ggtgtcttct atggaggtca aaacagcgtg gatggcgtct ccaggcgatc tgacggttca
      181 ctaaacgagc tcgtcgacga tctctatcac tgatagggag atctctatca ctgataggga
      241 gagctctgct tatatagacc tcccaccgta cacgcctacc gcccatttgc gtcaatgggg
      301 cggagttgtt acgacatttt ggaaagtccc gttgattttg gtgccaaaac aaactcccat
      361 tgacgtcaat ggggtggaga cttggaaatc cccgtgagtc aaaccgctat ccacgcccat
      421 tgatgtactg ccaaaaccgc atcaccatgg taatagcgat gactaatacg tagatgtact
      481 gccaagtagg aaagtcccat aaggtcatgt actgggcata atgccaggcg ggccatttac
      541 cgtcattgac gtcaataggg ggcgtacttg gcatatgata cacttgatgt actgccaagt
      601 gggcagttta ccgtaaatac tccacccatt gacgtcaatg gaaagtccct attggcgtta
      661 ctatgggaac atacgtcatt attgacgtca atgggcgggg gtcgttgggc ggtcagccag
      721 gcgggccatt taccgtaagt tatgtaacgc ggaactccat atatgggcta tgaactaatg
      781 accccgtaat tgattactat taataactag tcaataatca atgtcaacgc ggatatctgg
      841 cccgtacagg atctcccgat
//
