LOCUS       sequence-69169-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For constitutive or doxycycline-inducible expression of mutant human
            Ku70 unable to bind DNA and resistant to siRNA. Confers resistance 
            to puro. Use T-REx cells for doxycycline-inducible expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Britton S, Coates J, Jackson SP
  TITLE     A new method for high-resolution imaging of Ku foci to decipher 
            mechanisms of DNA double-strand break repair.
  JOURNAL   J Cell Biol. 2013 Jul 29.
  PUBMED    23897892
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2013 Jul 29."
COMMENT     Sequence Label: pICE-EGFP-FLAG-Ku70siR-Mut6E
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             32..55
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ggacgagctg tacaagtccg gactcagatc tgattacaag gatgacgacg ataagctcga
       61 gtcagggtgg gagtcatatt acaaaaccga gggcgatgaa gaagcagagg aagaacaaga
      121 agagaacctt gaagcaagtg gagactataa atattcagga agagatagtt tgattttttt
      181 ggttgatgcc tccaaggcta tgtttgaatc tcaatccgag gacgaactca ccccttttga
      241 catgagcatc cagtgtatcc aaagtgtgta catcagtaag atcataagca gtgatcgaga
      301 tctcttggct gtggtgttct atggtaccga gaaagacaaa aattcagtga attttaaaaa
      361 tatttacgtc ttacaggagc tggataatcc aggtgcaaaa cgaattctag agcttgacca
      421 gtttaagggg cagcagggac aaaaacgttt ccaagacatg atgggccacg gatctgacta
      481 ctcactcagt gaagtgctgt gggtctgtgc caacctcttt agtgatgtcc aattcaagat
      541 gagtcataag aggatcatgc tgttcaccaa tgaagacaac ccccatggca atgacagtgc
      601 caaagccagc cgggccagga ccaaagccgg tgatctccga gatacaggca tcttccttga
      661 cttgatgcac ctgaagaaac ctgggggctt tgacatatcc ttgttctaca gagatatcat
      721 cagcatagca gaggatgagg acctcagggt tcactttgag gaatccagca agctagaaga
      781 cctgttgcgg aaggttcgcg ccaaggagac aaggaagcga gcactcagca ggttaaagct
      841 gaagctcaac aaagatatag tgatctctgt ggg
//
