LOCUS       sequence-69177-e         439 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for cloning and expression of GFP-FLAG tagged proteins 
            (N-terminal tag). Confers resistance to G418..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Britton S, Coates J, Jackson SP
  TITLE     A new method for high-resolution imaging of Ku foci to decipher 
            mechanisms of DNA double-strand break repair.
  JOURNAL   J Cell Biol. 2013 Jul 29.
  PUBMED    23897892
REFERENCE   2  (bases 1 to 439)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2013 Jul 29."
COMMENT     Sequence Label: pEGFP-C1-FLAG
FEATURES             Location/Qualifiers
     source          1..439
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(193..216)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(274..295)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
       61 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atcagttatc
      121 tagatccggt ggatcccggg cccgcggtac cgtcgactgc agaattcgaa gctttccgcg
      181 gccgccctcg agcttatcgt cgtcatcctt gtaatcagat ctgagtccgg acttgtacag
      241 ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac tccagcagga ccatgtgatc
      301 gcgcttctcg ttggggtctt tgctcagggc ggactgggtg ctcaggtagt ggttgtcggg
      361 cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg cgagctgcac
      421 gctgccgtcc tcgatgttg
//
