LOCUS       sequence-69201-e         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For constitutive or doxycycline-inducible expression of mutant human
            Ku70 unable to bind DNA and resistant to siRNA. Confers resistance 
            to puro. Use T-REx cells for doxycycline-inducible expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Britton S, Coates J, Jackson SP
  TITLE     A new method for high-resolution imaging of Ku foci to decipher 
            mechanisms of DNA double-strand break repair.
  JOURNAL   J Cell Biol. 2013 Jul 29.
  PUBMED    23897892
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2013 Jul 29."
COMMENT     Sequence Label: pICE-EGFP-FLAG-Ku70siR-Mut6E
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ggaagcgagc actcagcagg ttaaagctga agctcaacaa agatatagtg atctctgtgg
       61 gcatttataa tctggtccag aaggctctcg aacctcctcc aatagagctc tatcgggaaa
      121 caaatgaacc agtgaaaacc aaggagcgga cctttaatac aagtacaggc ggtttgcttc
      181 tgcctagcga taccaagagg tctcagatct atgggagtcg tcagattata ctggaggaag
      241 aggaaacaga agagctagaa cggtttgatg atccaggttt gatgctcatg ggtttcaagc
      301 cgttggtact gctgaagaaa caccattacc tgaggccctc cctgttcgtg tacccagagg
      361 agtcgctggt gattgggagc tcaaccctgt tcagtgctct gctcatcaag tgtctggaga
      421 aggaggttgc agcattgtgc agatacacac ccgagaggaa catccctcct tattttgtgg
      481 ctttggtgcc acaggaagaa gagttggatg accagaaaat tcaggtgact cctccaggct
      541 tccagctggt ctttttaccc tttgctgatg ataaaaggaa gatgcccttt actgaaaaaa
      601 tcatggcaac tccagagcag gtgggcaaga tgaaggctat cgttgagaag cttcgcttca
      661 catacagaag tgacagcttt gagaaccccg tgctgcagca gcacttcagg aacctggagg
      721 ccttggcctt ggatttgatg gagccggaac aagcagtgga cctgacattg cccaaggttg
      781 aagcaatgaa taaaagactg ggctccttgg tggatgagtt taa
//
