LOCUS       sequence-69224-e         979 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Codes for the SSA-TLR with the target site for I-Sce I in a 
            lentiviral backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Kuhar R, Gwiazda KS, Humbert O, Mandt T, Pangallo J, Brault M, Khan 
            I, Maizels N, Rawlings DJ, Scharenberg AM, Certo MT
  TITLE     Novel fluorescent genome editing reporters for monitoring DNA repair
            pathway utilization at endonuclease-induced breaks.
  JOURNAL   Nucleic Acids Res. 2013 Oct 10.
  PUBMED    24121685
REFERENCE   2  (bases 1 to 979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Oct 10."
COMMENT     Sequence Label: pCVL.SSA TLR (Sce)
FEATURES             Location/Qualifiers
     source          1..979
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     233..254
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(297..350)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg
       61 tggtcggggt agcggctgaa gcactgcacg ccgtaggtca gggttcacac tagttaggga
      121 taacagggta atacctgcag gttgccggtg gtgcagatga acttcagggt cagcttgccg
      181 taggtggcat cgccctcgcc ctcgccggac acgctgaact tgtggccgtt tacgtcgccg
      241 tccagctcga ccaggatggg caccaccccg gtgaacagct cctcgccctt gctcacgggg
      301 cccggattct cctccacgtc accgcatgtt agaagacttc ctctgccctc tctagaccac
      361 agtcgctcgc cgcgcaaaat cgagatcgac atcgtgccgt gcatgcctat gttgcgcatg
      421 aattccagat ggacgggcga gacgctgcgc aggatggcga agctaagatc aatcggccgc
      481 ccggtgaccg gattgaggtc tggggtgacc ggcaccggcc gataattgat atcgggaatg
      541 atccgtaccg ggttgatggt atagagccga cgggcctgcg ccggcacggt tgaggcagga
      601 tagtgcaggc ctagttttga ctcgacctcg gcgcaccgat cctctgcgat cacttcgccg
      661 ctgaagtcgg aggcgaagcg atagatcatc acccgatcga agccggtaat cttccgcacc
      721 tcttgcgccg cggcggcgca ggcgctttcc aaggtttcgg cggcctgcag gcggcggatg
      781 gcgctgttgg tgcggcggaa gaacgcctgc ggctcggcga cgtcccgctg gggaggctcg
      841 agctcgagga agatgagctg atcatggcga tgccaggagc cgatgaagcc tgcgtccttt
      901 cgcatcgtga agccgacagt gatcggtgct ccgacggccg ccccgggctc ggccaaggcg
      961 atcgtcagac ggttgtgcg
//
