LOCUS       sequence-69225-e         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Spangle JM, Munger K
  TITLE     The human papillomavirus type 16 E6 oncoprotein activates mTORC1 
            signaling and increases protein synthesis.
  JOURNAL   J Virol. 2010 Sep;84(18):9398-407. Epub 2010 Jul 14.
  PUBMED    20631133
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol."; 
            date: "2010-09"; volume: "84(18)"; pages: "9398-407. Epub 2010 Jul 
            14"
COMMENT     Sequence Label: pNCMV
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          48..620
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     CDS             690..713
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             726..752
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 accgggaccg atccagcctc cggtcgaccg atcctgagaa cttcagggtg agtttgggga
       61 cccttgattg ttctttcttt ttcgctattg taaaattcat gttatatgga gggggcaaag
      121 ttttcagggt gttgtttaga atgggaagat gtcccttgta tcaccatgga ccctcatgat
      181 aattttgttt ctttcacttt ctactctgtt gacaaccatt gtctcctctt attttctttt
      241 cattttctgt aactttttcg ttaaacttta gcttgcattt gtaacgaatt tttaaattca
      301 cttttgttta tttgtcagat tgtaagtact ttctctaatc actttttttt caaggcaatc
      361 agggtatatt atattgtact tcagcacagt tttagagaac aattgttata attaaatgat
      421 aaggtagaat atttctgcat ataaattctg gctggcgtgg aaatattctt attggtagaa
      481 acaactacac cctggtcatc atcctgcctt tctctttatg gttacaatga tatacactgt
      541 ttgagatgag gataaaatac tctgagtcca aaccgggccc ctctgctaac catgttcatg
      601 ccttcttctc tttcctacag ctcctgggca acgtgctggt tgttgtgctg tctcatcatt
      661 ttggcaaaga attcctcgac ggatctatgg actacaagga tgacgatgac aagctcgatg
      721 gaggataccc atacgatgtt ccagattacg ctgatatcgg atccgtcgag gaattcactc
      781 ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc ctgg
//
