LOCUS       sequence-69232-e       10180 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Destination plasmid (Gateway cloning) for cumate-inducible 
            expression of 3xFLAG-tagged fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10180)
  AUTHORS   Kaczmarczyk A, Vorholt JA, Francez-Charlot A
  TITLE     Cumate-inducible gene expression system for sphingomonads and other 
            Alphaproteobacteria.
  JOURNAL   Appl Environ Microbiol. 2013 Nov;79(21):6795-802. doi: 
            10.1128/AEM.02296-13. Epub 2013 Aug 30.
  PUBMED    23995928
REFERENCE   2  (bases 1 to 10180)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.02296-13"; journalName: "Appl Environ Microbiol"; date:
            "2013-11"; volume: "79"; issue: "21"; pages: "6795-802"
COMMENT     Sequence Label: pQFD
FEATURES             Location/Qualifiers
     source          1..10180
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      11..642
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      1105..1693
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1755..2366)
                     /codon_start=1
                     /gene="cymR from Pseudomonas putida"
                     /product="cumate repressor (Mullick et al., 2006)"
                     /label=CymR
                     /translation="MSPKRRTQAERAMETQGKLIAAALGVLREKGYAGFRIADVPGAAG
                     VSRGAQSHHFPTKLELLLATFEWLYEQITERSRARLAKLKPEDDVIQQMLDDAAEFFLD
                     DDFSISLDLIVAADRDPALREGIQRTVERNRFVVEDMWLGVLVSRGLSRDDAEDILWLI
                     FNSVRGLAVRSLWQKDKERFERVRNSTLEIARERYAKFKR"
     protein_bind    2443..2470
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2513..2540
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     CDS             2565..2630
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    2652..2776
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2801..2806,2807..2824,2825..2831)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2885..3544
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2951..2970)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      3597..4185
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4339..4356
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             4818..5123
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5002..5021
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5164..5288)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             5330..5395
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(5467..5484)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5467..5483)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5476..5498)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5692..5711)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5811..5833
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5871..5889)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             6675..7874
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(8105..9253)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(9525..9896)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            9929..10038
                     /note="incP origin of transfer"
ORIGIN
        1 gaccctttcc gacgctcacc gggctggttg ccctcgccgc tgggctggcg gccgtctatg
       61 gccctgcaaa cgcgccagaa acgccgtcga agccgtgtgc gagacaccgc ggccgccggc
      121 gttgtggata cctcgcggaa aacttggccc tcactgacag atgaggggcg gacgttgaca
      181 cttgaggggc cgactcaccc ggcgcggcgt tgacagatga ggggcaggct cgatttcggc
      241 cggcgacgtg gagctggcca gcctcgcaaa tcggcgaaaa cgcctgattt tacgcgagtt
      301 tcccacagat gatgtggaca agcctgggga taagtgccct gcggtattga cacttgaggg
      361 gcgcgactac tgacagatga ggggcgcgat ccttgacact tgaggggcag agtgctgaca
      421 gatgaggggc gcacctattg acatttgagg ggctgtccac aggcagaaaa tccagcattt
      481 gcaagggttt ccgcccgttt ttcggccacc gctaacctgt cttttaacct gcttttaaac
      541 caatatttat aaaccttgtt tttaaccagg gctgcgccct gtgcgcgtga ccgcgcacgc
      601 cgaagggggg tgccccccct tctcgaaccc tcccggcccg ctaacgcggg cctcccatcc
      661 ccccaggggc tgcgcccctc ggccgcgaac ggcctcaccc caaaaatggc agccaagctg
      721 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
      781 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
      841 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
      901 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
      961 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     1021 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     1081 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     1141 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     1201 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     1261 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     1321 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     1381 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     1441 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     1501 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     1561 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     1621 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     1681 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     1741 ttttgctcac atgttcagcg cttgaacttg gcgtagcgct cgcgggcgat ttccagggtc
     1801 gagttgcgga cgcgctcgaa gcgctccttg tccttctgcc acagcgagcg gaccgccagg
     1861 ccgcggaccg agttgaagat cagccacagg atgtcctccg cgtcgtcgcg gctcaggccg
     1921 cgcgacacca gcacgcccag ccacatatcc tcgacgacga agcggttgcg ctcgacggtg
     1981 cgctggatgc cctcgcgcag cgccgggtcg cggtcggcgg ccacgatcag gtccagcgag
     2041 atcgagaagt cgtcgtccag gaagaactcg gcggcgtcgt ccagcatctg ctggatgacg
     2101 tcgtcctcgg gcttcagctt ggccaggcgg gcgcggctgc gctcggtgat ctgctcgtac
     2161 agccactcga aggtggccag cagcagttcc agcttggtcg ggaagtggtg cgactgggcg
     2221 ccgcggctga cgccggcggc gcccgggacg tcggcgatgc ggaagccggc atagcccttc
     2281 tcgcgcagga cgcccagggc ggcggcgatc agcttgccct gggtttccat cgcgcgctcg
     2341 gcctgggtgc ggcgcttcgg cgacatgatg accattcggg gtctctccct gctagcatca
     2401 gggttattgt atcatgagcg gatacatatg tcaatgtacc ggaacaaaca gacaatctgg
     2461 tctgtttgta catggttgac ggatcgccgc gaagccgcta actgcgccgg ttaacaaaca
     2521 gacaatctgg tctgtttgta actagtagag gaagcttccg catggactac aaggaccacg
     2581 acggcgacta taaggaccac gacatcgact acaaggatga cgacgacaag cttattaata
     2641 tgactctaga gacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat gatataaata
     2701 tcaatatatt aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac
     2761 atatccagtc actatggcgg ccgcattagg caccccaggc tttacacttt atgcttccgg
     2821 ctcgtataat gtgtggattt tgagttagga tccggcgaga ttttcaggag ctaaggaagc
     2881 taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa
     2941 agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct
     3001 ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt
     3061 tattcacatt cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga
     3121 cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac
     3181 tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat
     3241 atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat
     3301 tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa
     3361 cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca
     3421 aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgtct gtgatggctt
     3481 ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc
     3541 gtaaacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     3601 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     3661 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     3721 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     3781 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     3841 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     3901 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     3961 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     4021 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     4081 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     4141 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     4201 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     4261 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     4321 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     4381 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     4441 gactggaaag cgggcagtga gcgcaacgca attaatacgc gtggatccgg cttactaaaa
     4501 gccagataac agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat
     4561 atgtataccc gaagtatgtc aaaaagaggt gtgctatgaa gcagcgtatt acagtgacag
     4621 ttgacagcga cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt
     4681 aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa
     4741 tcaggaaggg atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga
     4801 gaacagggac tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc
     4861 gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc
     4921 ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc
     4981 atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg
     5041 ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta
     5101 acctgatgtt ctggggaata taaatgtcag gctccgttat acacagccag tctgcaggtc
     5161 gaccatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa
     5221 atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac
     5281 aaagtggtga tatcgctagc tagaggtacc gagctccaat tgggatccgg actacaagga
     5341 ccacgacggc gactataagg accacgacat cgactacaag gatgacgacg acaagtgagg
     5401 atccggtgaa gtgacccgca aaaaaaaagg ccaggggatc gctcccccgg cctttttttt
     5461 gaattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     5521 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     5581 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt
     5641 tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg
     5701 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     5761 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     5821 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
     5881 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagcacc ctttctcggt
     5941 ccttcaacgt tcctgacaac gagcctcctt ttcgccaatc catcgacaat caccgcgagt
     6001 ccctgctcga acgctgcgtc cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac
     6061 agcggcgaga gcggagcctg ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg
     6121 gcctgctcct caagcacggc cccaacagtg aagtagctga ttgtcatcag cgcattgacg
     6181 gcgtccccgg ccgaaaaacc cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc
     6241 atctgcggtg cgcccggtcg cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc
     6301 agcgcctgcc tgaagctgcg ggcattcccg atcagaaatg agcgccagtc gtcgtcggct
     6361 ctcggcaccg aatgcgtatg attctccgcc agcatggctt cggccagtgc gtcgagcagc
     6421 gcccgcttgt tcctgaagtg ccagtaaagc gccggctgct gaacccccaa ccgttccgcc
     6481 agtttgcgtg tcgtcagacc gtctacgccg acctcgttca acaggtccag ggcggcacgg
     6541 atcactgtat tcggctgcaa ctttgtcatg attgacactt tatcactgat aaacataata
     6601 tgtccaccaa cttatcagtg ataaagaatc cgcgcgttca atcggaccag cggaggctgg
     6661 tccggaggcc agacgtgaaa cccaacatac ccctgatcgt aattctgagc actgtcgcgc
     6721 tcgacgctgt cggcatcggc ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg
     6781 ttcactcgaa cgacgtcacc gcccactatg gcattctgct ggcgctgtat gcgttggtgc
     6841 aatttgcctg cgcacctgtg ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct
     6901 tgctcgtctc gctggccggc gccactgtcg actacgccat catggcgaca gcgcctttcc
     6961 tttgggttct ctatatcggg cggatcgtgg ccggcatcac cggggcgact ggggcggtag
     7021 ccggcgctta tattgccgat atcactgatg gcgatgagcg cgcgcggcac ttcggcttca
     7081 tgagcgcctg tttcgggttc gggatggtcg cgggacctgt gctcggtggg ctgatgggcg
     7141 gtttctcccc ccacgctccg ttcttcgccg cggcagcctt gaacggcctc aatttcctga
     7201 cgggctgttt ccttttgccg gagtcgcaca aaggcgaacg ccggccgtta cgccgggagg
     7261 ctctcaaccc gctcgcttcg ttccggtggg cccggggcat gaccgtcgtc gccgccctga
     7321 tggcggtctt cttcatcatg caacttgtcg gacaggtgcc ggccgcgctt tgggtcattt
     7381 tcggcgagga tcgctttcac tgggacgcga ccacgatcgg catttcgctt gccgcatttg
     7441 gcattctgca ttcactcgcc caggcaatga tcaccggccc tgtagccgcc cggctcggcg
     7501 aaaggcgggc actcatgctc ggaatgattg ccgacggcac aggctacatc ctgcttgcct
     7561 tcgcgacacg gggatggatg gcgttcccga tcatggtcct gcttgcttcg ggtggcatcg
     7621 gaatgccggc gctgcaagca atgttgtcca ggcaggtgga tgaggaacgt caggggcagc
     7681 tgcaaggctc actggcggcg ctcaccagcc tgacctcgat cgtcggaccc ctcctcttca
     7741 cggcgatcta tgcggcttct ataacaacgt ggaacgggtg ggcatggatt gcaggcgctg
     7801 ccctctactt gctctgcctg ccggcgctgc gtcgcgggct ttggagcggc gcagggcaac
     7861 gagccgatcg ctgatcgtgg aaacgatagg cctatgccat gcgggtcaag gcgacttccg
     7921 gcaagctata cgcgccctag aattgtcaat tttaatcctc tgtttatcgg cagttcgtag
     7981 agcgcgccgt gcgtcccgag cgatactgag cgaagcaagt gcgtcgagca gtgcccgctt
     8041 gttcctgaaa tgccagtaaa gcgctggctg ctgaaccccc agccggaact gaccccacaa
     8101 ggccctagcg tttgcaatgc accaggtcat cattgaccca ggcgtgttcc accaggccgc
     8161 tgcctcgcaa ctcttcgcag gcttcgccga cctgctcgcg ccacttcttc acgcgggtgg
     8221 aatccgatcc gcacatgagg cggaaggttt ccagcttgag cgggtacggc tcccggtgcg
     8281 agctgaaata gtcgaacatc cgtcgggccg tcggcgacag cttgcggtac ttctcccata
     8341 tgaatttcgt gtagtggtcg ccagcaaaca gcacgacgat ttcctcgtcg atcaggacct
     8401 ggcaacggga cgttttcttg ccacggtcca ggacgcggaa gcggtgcagc agcgacaccg
     8461 attccaggtg cccaacgcgg tcggacgtga agcccatcgc cgtcgcctgt aggcgcgaca
     8521 ggcattcctc ggccttcgtg taataccggc cattgatcga ccagcccagg tcctggcaaa
     8581 gctcgtagaa cgtgaaggtg atcggctcgc cgataggggt gcgcttcgcg tactccaaca
     8641 cctgctgcca caccagttcg tcatcgtcgg cccgcagctc gacgccggtg taggtgatct
     8701 tcacgtcctt gttgacgtgg aaaatgacct tgttttgcag cgcctcgcgc gggattttct
     8761 tgttgcgcgt ggtgaacagg gcagagcggg ccgtgtcgtt tggcatcgct cgcatcgtgt
     8821 ccggccacgg cgcaatatcg aacaaggaaa gctgcatttc cttgatctgc tgcttcgtgt
     8881 gtttcagcaa cgcggcctgc ttggcctcgc tgacctgttt tgccaggtcc tcgccggcgg
     8941 tttttcgctt cttggtcgtc atagttcctc gcgtgtcgat ggtcatcgac ttcgccaaac
     9001 ctgccgcctc ctgttcgaga cgacgcgaac gctccacggc ggccgatggc gcgggcaggg
     9061 cagggggagc cagttgcacg ctgtcgcgct cgatcttggc cgtagcttgc tggaccatcg
     9121 agccgacgga ctggaaggtt tcgcggggcg cacgcatgac ggtgcggctt gcgatggttt
     9181 cggcatcctc ggcggaaaac cccgcgtcga tcagttcttg cctgtatgcc ttccggtcaa
     9241 acgtccgatt cattcaccct ccttgcggga ttgccccgac tcacgccggg gcaatgtgcc
     9301 cttattcctg atttgacccg cctggtgcct tggtgtccag ataatccacc ttatcggcaa
     9361 tgaagtcggt cccgtagacc gtctggccgt ccttctcgta cttggtattc cgaatcttgc
     9421 cctgcacgaa taccagctcc gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct
     9481 tggcaatcac gcgcaccccc cggccgtttt agcggctaaa aaagtcatgg ctctgccctc
     9541 gggcggacca cgcccatcat gaccttgcca agctcgtcct gcttctcttc gatcttcgcc
     9601 agcagggcga ggatcgtggc atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag
     9661 agtttcagca ggccgcccag gcggcccagg tcgccattga tgcgggccag ctcgcggacg
     9721 tgctcatagt ccacgacgcc cgtgattttg tagccctggc cgacggccag caggtaggcc
     9781 tacaggctca tgccggccgc cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg
     9841 cagtacacct tgataggtgg gctgcccttc ctggttggct tggtttcatc agccatccgc
     9901 ttgccctcat ctgttacgcc ggcggtagcc ggccagcctc gcagagcagg attcccgttg
     9961 agcaccgcca ggtgcgaata agggacagtg aagaaggaac acccgctcgc gggtgggcct
    10021 acttcaccta tcctgcccgg ctgacgccgt tggatacacc aaggaaagtc tacacgaacc
    10081 ctttggcaaa atcctgtata tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata
    10141 atgaccccga agcagggtta tgcagcggaa aagatccgtc
//
