LOCUS       sequence-69234-e       10147 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Destination plasmid (Gateway cloning) for cumate-inducible 
            expression of myc-tagged fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10147)
  AUTHORS   Kaczmarczyk A, Vorholt JA, Francez-Charlot A
  TITLE     Cumate-inducible gene expression system for sphingomonads and other 
            Alphaproteobacteria.
  JOURNAL   Appl Environ Microbiol. 2013 Nov;79(21):6795-802. doi: 
            10.1128/AEM.02296-13. Epub 2013 Aug 30.
  PUBMED    23995928
REFERENCE   2  (bases 1 to 10147)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.02296-13"; journalName: "Appl Environ Microbiol"; date:
            "2013-11"; volume: "79"; issue: "21"; pages: "6795-802"
COMMENT     Sequence Label: pQMD
FEATURES             Location/Qualifiers
     source          1..10147
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      11..642
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      1105..1693
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1755..2366)
                     /codon_start=1
                     /gene="cymR from Pseudomonas putida"
                     /product="cumate repressor (Mullick et al., 2006)"
                     /label=CymR
                     /translation="MSPKRRTQAERAMETQGKLIAAALGVLREKGYAGFRIADVPGAAG
                     VSRGAQSHHFPTKLELLLATFEWLYEQITERSRARLAKLKPEDDVIQQMLDDAAEFFLD
                     DDFSISLDLIVAADRDPALREGIQRTVERNRFVVEDMWLGVLVSRGLSRDDAEDILWLI
                     FNSVRGLAVRSLWQKDKERFERVRNSTLEIARERYAKFKR"
     protein_bind    2443..2470
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2513..2540
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     CDS             2565..2594
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    2619..2743
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2768..2773,2774..2791,2792..2798)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2852..3511
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2918..2937)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      3564..4152
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4306..4323
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             4785..5090
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4969..4988
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5131..5255)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             5297..5362
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(5434..5451)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5434..5450)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5443..5465)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5659..5678)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5778..5800
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5838..5856)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             6642..7841
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(8072..9220)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(9492..9863)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            9896..10005
                     /note="incP origin of transfer"
ORIGIN
        1 gaccctttcc gacgctcacc gggctggttg ccctcgccgc tgggctggcg gccgtctatg
       61 gccctgcaaa cgcgccagaa acgccgtcga agccgtgtgc gagacaccgc ggccgccggc
      121 gttgtggata cctcgcggaa aacttggccc tcactgacag atgaggggcg gacgttgaca
      181 cttgaggggc cgactcaccc ggcgcggcgt tgacagatga ggggcaggct cgatttcggc
      241 cggcgacgtg gagctggcca gcctcgcaaa tcggcgaaaa cgcctgattt tacgcgagtt
      301 tcccacagat gatgtggaca agcctgggga taagtgccct gcggtattga cacttgaggg
      361 gcgcgactac tgacagatga ggggcgcgat ccttgacact tgaggggcag agtgctgaca
      421 gatgaggggc gcacctattg acatttgagg ggctgtccac aggcagaaaa tccagcattt
      481 gcaagggttt ccgcccgttt ttcggccacc gctaacctgt cttttaacct gcttttaaac
      541 caatatttat aaaccttgtt tttaaccagg gctgcgccct gtgcgcgtga ccgcgcacgc
      601 cgaagggggg tgccccccct tctcgaaccc tcccggcccg ctaacgcggg cctcccatcc
      661 ccccaggggc tgcgcccctc ggccgcgaac ggcctcaccc caaaaatggc agccaagctg
      721 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
      781 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
      841 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
      901 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
      961 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     1021 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     1081 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     1141 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     1201 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     1261 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     1321 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     1381 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     1441 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     1501 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     1561 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     1621 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     1681 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     1741 ttttgctcac atgttcagcg cttgaacttg gcgtagcgct cgcgggcgat ttccagggtc
     1801 gagttgcgga cgcgctcgaa gcgctccttg tccttctgcc acagcgagcg gaccgccagg
     1861 ccgcggaccg agttgaagat cagccacagg atgtcctccg cgtcgtcgcg gctcaggccg
     1921 cgcgacacca gcacgcccag ccacatatcc tcgacgacga agcggttgcg ctcgacggtg
     1981 cgctggatgc cctcgcgcag cgccgggtcg cggtcggcgg ccacgatcag gtccagcgag
     2041 atcgagaagt cgtcgtccag gaagaactcg gcggcgtcgt ccagcatctg ctggatgacg
     2101 tcgtcctcgg gcttcagctt ggccaggcgg gcgcggctgc gctcggtgat ctgctcgtac
     2161 agccactcga aggtggccag cagcagttcc agcttggtcg ggaagtggtg cgactgggcg
     2221 ccgcggctga cgccggcggc gcccgggacg tcggcgatgc ggaagccggc atagcccttc
     2281 tcgcgcagga cgcccagggc ggcggcgatc agcttgccct gggtttccat cgcgcgctcg
     2341 gcctgggtgc ggcgcttcgg cgacatgatg accattcggg gtctctccct gctagcatca
     2401 gggttattgt atcatgagcg gatacatatg tcaatgtacc ggaacaaaca gacaatctgg
     2461 tctgtttgta catggttgac ggatcgccgc gaagccgcta actgcgccgg ttaacaaaca
     2521 gacaatctgg tctgtttgta actagtagag gaagcttccg catggagcag aagctgatct
     2581 cggaagagga cctgggctcg attaatatga ctctagagac aagtttgtac aaaaaagctg
     2641 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac
     2701 agactacata atactgtaaa acacaacata tccagtcact atggcggccg cattaggcac
     2761 cccaggcttt acactttatg cttccggctc gtataatgtg tggattttga gttaggatcc
     2821 ggcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac
     2881 cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca
     2941 atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa
     3001 aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca
     3061 tccggaattc cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc
     3121 ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca
     3181 cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa
     3241 cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg
     3301 ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt
     3361 tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca
     3421 ggttcatcat gccgtctgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca
     3481 gtactgcgat gagtggcagg gcggggcgta aacgcgtcgt tccactgagc gtcagacccc
     3541 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     3601 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     3661 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     3721 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     3781 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     3841 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     3901 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     3961 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     4021 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     4081 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     4141 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     4201 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     4261 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     4321 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     4381 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     4441 aatacgcgtg gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat
     4501 ttttgcggta taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtgtg
     4561 ctatgaagca gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat
     4621 atatgatgtc aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct
     4681 gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat
     4741 tgaaatgaac ggctcttttg ctgacgagaa cagggactgg tgaaatgcag tttaaggttt
     4801 acacctataa aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg
     4861 acacgcccgg gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag
     4921 tctcccgtga actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca
     4981 ccgatatggc cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc
     5041 gcgaaaatga catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct
     5101 ccgttataca cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag
     5161 tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt
     5221 tacgtttctc gttcagcttt cttgtacaaa gtggtgatat cgctagctag aggtaccgag
     5281 ctccaattgg gatccggact acaaggacca cgacggcgac tataaggacc acgacatcga
     5341 ctacaaggat gacgacgaca agtgaggatc cggtgaagtg acccgcaaaa aaaaaggcca
     5401 ggggatcgct cccccggcct tttttttgaa ttcactggcc gtcgttttac aacgtcgtga
     5461 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     5521 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     5581 tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     5641 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     5701 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     5761 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     5821 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     5881 aatggtttct tagcaccctt tctcggtcct tcaacgttcc tgacaacgag cctccttttc
     5941 gccaatccat cgacaatcac cgcgagtccc tgctcgaacg ctgcgtccgg accggcttcg
     6001 tcgaaggcgt ctatcgcggc ccgcaacagc ggcgagagcg gagcctgttc aacggtgccg
     6061 ccgcgctcgc cggcatcgct gtcgccggcc tgctcctcaa gcacggcccc aacagtgaag
     6121 tagctgattg tcatcagcgc attgacggcg tccccggccg aaaaacccgc ctcgcagagg
     6181 aagcgaagct gcgcgtcggc cgtttccatc tgcggtgcgc ccggtcgcgt gccggcatgg
     6241 atgcgcgcgc catcgcggta ggcgagcagc gcctgcctga agctgcgggc attcccgatc
     6301 agaaatgagc gccagtcgtc gtcggctctc ggcaccgaat gcgtatgatt ctccgccagc
     6361 atggcttcgg ccagtgcgtc gagcagcgcc cgcttgttcc tgaagtgcca gtaaagcgcc
     6421 ggctgctgaa cccccaaccg ttccgccagt ttgcgtgtcg tcagaccgtc tacgccgacc
     6481 tcgttcaaca ggtccagggc ggcacggatc actgtattcg gctgcaactt tgtcatgatt
     6541 gacactttat cactgataaa cataatatgt ccaccaactt atcagtgata aagaatccgc
     6601 gcgttcaatc ggaccagcgg aggctggtcc ggaggccaga cgtgaaaccc aacatacccc
     6661 tgatcgtaat tctgagcact gtcgcgctcg acgctgtcgg catcggcctg attatgccgg
     6721 tgctgccggg cctcctgcgc gatctggttc actcgaacga cgtcaccgcc cactatggca
     6781 ttctgctggc gctgtatgcg ttggtgcaat ttgcctgcgc acctgtgctg ggcgcgctgt
     6841 cggatcgttt cgggcggcgg ccaatcttgc tcgtctcgct ggccggcgcc actgtcgact
     6901 acgccatcat ggcgacagcg cctttccttt gggttctcta tatcgggcgg atcgtggccg
     6961 gcatcaccgg ggcgactggg gcggtagccg gcgcttatat tgccgatatc actgatggcg
     7021 atgagcgcgc gcggcacttc ggcttcatga gcgcctgttt cgggttcggg atggtcgcgg
     7081 gacctgtgct cggtgggctg atgggcggtt tctcccccca cgctccgttc ttcgccgcgg
     7141 cagccttgaa cggcctcaat ttcctgacgg gctgtttcct tttgccggag tcgcacaaag
     7201 gcgaacgccg gccgttacgc cgggaggctc tcaacccgct cgcttcgttc cggtgggccc
     7261 ggggcatgac cgtcgtcgcc gccctgatgg cggtcttctt catcatgcaa cttgtcggac
     7321 aggtgccggc cgcgctttgg gtcattttcg gcgaggatcg ctttcactgg gacgcgacca
     7381 cgatcggcat ttcgcttgcc gcatttggca ttctgcattc actcgcccag gcaatgatca
     7441 ccggccctgt agccgcccgg ctcggcgaaa ggcgggcact catgctcgga atgattgccg
     7501 acggcacagg ctacatcctg cttgccttcg cgacacgggg atggatggcg ttcccgatca
     7561 tggtcctgct tgcttcgggt ggcatcggaa tgccggcgct gcaagcaatg ttgtccaggc
     7621 aggtggatga ggaacgtcag gggcagctgc aaggctcact ggcggcgctc accagcctga
     7681 cctcgatcgt cggacccctc ctcttcacgg cgatctatgc ggcttctata acaacgtgga
     7741 acgggtgggc atggattgca ggcgctgccc tctacttgct ctgcctgccg gcgctgcgtc
     7801 gcgggctttg gagcggcgca gggcaacgag ccgatcgctg atcgtggaaa cgataggcct
     7861 atgccatgcg ggtcaaggcg acttccggca agctatacgc gccctagaat tgtcaatttt
     7921 aatcctctgt ttatcggcag ttcgtagagc gcgccgtgcg tcccgagcga tactgagcga
     7981 agcaagtgcg tcgagcagtg cccgcttgtt cctgaaatgc cagtaaagcg ctggctgctg
     8041 aacccccagc cggaactgac cccacaaggc cctagcgttt gcaatgcacc aggtcatcat
     8101 tgacccaggc gtgttccacc aggccgctgc ctcgcaactc ttcgcaggct tcgccgacct
     8161 gctcgcgcca cttcttcacg cgggtggaat ccgatccgca catgaggcgg aaggtttcca
     8221 gcttgagcgg gtacggctcc cggtgcgagc tgaaatagtc gaacatccgt cgggccgtcg
     8281 gcgacagctt gcggtacttc tcccatatga atttcgtgta gtggtcgcca gcaaacagca
     8341 cgacgatttc ctcgtcgatc aggacctggc aacgggacgt tttcttgcca cggtccagga
     8401 cgcggaagcg gtgcagcagc gacaccgatt ccaggtgccc aacgcggtcg gacgtgaagc
     8461 ccatcgccgt cgcctgtagg cgcgacaggc attcctcggc cttcgtgtaa taccggccat
     8521 tgatcgacca gcccaggtcc tggcaaagct cgtagaacgt gaaggtgatc ggctcgccga
     8581 taggggtgcg cttcgcgtac tccaacacct gctgccacac cagttcgtca tcgtcggccc
     8641 gcagctcgac gccggtgtag gtgatcttca cgtccttgtt gacgtggaaa atgaccttgt
     8701 tttgcagcgc ctcgcgcggg attttcttgt tgcgcgtggt gaacagggca gagcgggccg
     8761 tgtcgtttgg catcgctcgc atcgtgtccg gccacggcgc aatatcgaac aaggaaagct
     8821 gcatttcctt gatctgctgc ttcgtgtgtt tcagcaacgc ggcctgcttg gcctcgctga
     8881 cctgttttgc caggtcctcg ccggcggttt ttcgcttctt ggtcgtcata gttcctcgcg
     8941 tgtcgatggt catcgacttc gccaaacctg ccgcctcctg ttcgagacga cgcgaacgct
     9001 ccacggcggc cgatggcgcg ggcagggcag ggggagccag ttgcacgctg tcgcgctcga
     9061 tcttggccgt agcttgctgg accatcgagc cgacggactg gaaggtttcg cggggcgcac
     9121 gcatgacggt gcggcttgcg atggtttcgg catcctcggc ggaaaacccc gcgtcgatca
     9181 gttcttgcct gtatgccttc cggtcaaacg tccgattcat tcaccctcct tgcgggattg
     9241 ccccgactca cgccggggca atgtgccctt attcctgatt tgacccgcct ggtgccttgg
     9301 tgtccagata atccacctta tcggcaatga agtcggtccc gtagaccgtc tggccgtcct
     9361 tctcgtactt ggtattccga atcttgccct gcacgaatac cagctccgcg aagtcgctct
     9421 tcttgatgga gcgcatgggg acgtgcttgg caatcacgcg caccccccgg ccgttttagc
     9481 ggctaaaaaa gtcatggctc tgccctcggg cggaccacgc ccatcatgac cttgccaagc
     9541 tcgtcctgct tctcttcgat cttcgccagc agggcgagga tcgtggcatc accgaaccgc
     9601 gccgtgcgcg ggtcgtcggt gagccagagt ttcagcaggc cgcccaggcg gcccaggtcg
     9661 ccattgatgc gggccagctc gcggacgtgc tcatagtcca cgacgcccgt gattttgtag
     9721 ccctggccga cggccagcag gtaggcctac aggctcatgc cggccgccgc cgccttttcc
     9781 tcaatcgctc ttcgttcgtc tggaaggcag tacaccttga taggtgggct gcccttcctg
     9841 gttggcttgg tttcatcagc catccgcttg ccctcatctg ttacgccggc ggtagccggc
     9901 cagcctcgca gagcaggatt cccgttgagc accgccaggt gcgaataagg gacagtgaag
     9961 aaggaacacc cgctcgcggg tgggcctact tcacctatcc tgcccggctg acgccgttgg
    10021 atacaccaag gaaagtctac acgaaccctt tggcaaaatc ctgtatatcg tgcgaaaaag
    10081 gatggatata ccgaaaaaat cgctataatg accccgaagc agggttatgc agcggaaaag
    10141 atccgtc
//
