LOCUS       sequence-69235-e       10825 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Destination plasmid (Gateway cloning) for cumate-inducible 
            expression of SYFP2-tagged fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10825)
  AUTHORS   Kaczmarczyk A, Vorholt JA, Francez-Charlot A
  TITLE     Cumate-inducible gene expression system for sphingomonads and other 
            Alphaproteobacteria.
  JOURNAL   Appl Environ Microbiol. 2013 Nov;79(21):6795-802. doi: 
            10.1128/AEM.02296-13. Epub 2013 Aug 30.
  PUBMED    23995928
REFERENCE   2  (bases 1 to 10825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.02296-13"; journalName: "Appl Environ Microbiol"; date:
            "2013-11"; volume: "79"; issue: "21"; pages: "6795-802"
COMMENT     Sequence Label: pQYD
FEATURES             Location/Qualifiers
     source          1..10825
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      11..642
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      1105..1693
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1755..2366)
                     /codon_start=1
                     /gene="cymR from Pseudomonas putida"
                     /product="cumate repressor (Mullick et al., 2006)"
                     /label=CymR
                     /translation="MSPKRRTQAERAMETQGKLIAAALGVLREKGYAGFRIADVPGAAG
                     VSRGAQSHHFPTKLELLLATFEWLYEQITERSRARLAKLKPEDDVIQQMLDDAAEFFLD
                     DDFSISLDLIVAADRDPALREGIQRTVERNRFVVEDMWLGVLVSRGLSRDDAEDILWLI
                     FNSVRGLAVRSLWQKDKERFERVRNSTLEIARERYAKFKR"
     protein_bind    2443..2470
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2513..2540
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     CDS             join(2562..2564,2565..2567,2568..3278)
                     /codon_start=1
                     /product="monomeric optimized variant of EYFP, identical to
                     mVenus but with L68V (Kremers et al., 2006)"
                     /label=SYFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2607..2628)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2868..2887)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3215..3236
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    3297..3421
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3446..3451,3452..3469,3470..3476)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3530..4189
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3596..3615)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      4242..4830
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4984..5001
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             5463..5768
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5647..5666
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5809..5933)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             5975..6040
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(6112..6129)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6112..6128)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6121..6143)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(6337..6356)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6456..6478
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6516..6534)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             7320..8519
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(8750..9898)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(10170..10541)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            10574..10683
                     /note="incP origin of transfer"
ORIGIN
        1 gaccctttcc gacgctcacc gggctggttg ccctcgccgc tgggctggcg gccgtctatg
       61 gccctgcaaa cgcgccagaa acgccgtcga agccgtgtgc gagacaccgc ggccgccggc
      121 gttgtggata cctcgcggaa aacttggccc tcactgacag atgaggggcg gacgttgaca
      181 cttgaggggc cgactcaccc ggcgcggcgt tgacagatga ggggcaggct cgatttcggc
      241 cggcgacgtg gagctggcca gcctcgcaaa tcggcgaaaa cgcctgattt tacgcgagtt
      301 tcccacagat gatgtggaca agcctgggga taagtgccct gcggtattga cacttgaggg
      361 gcgcgactac tgacagatga ggggcgcgat ccttgacact tgaggggcag agtgctgaca
      421 gatgaggggc gcacctattg acatttgagg ggctgtccac aggcagaaaa tccagcattt
      481 gcaagggttt ccgcccgttt ttcggccacc gctaacctgt cttttaacct gcttttaaac
      541 caatatttat aaaccttgtt tttaaccagg gctgcgccct gtgcgcgtga ccgcgcacgc
      601 cgaagggggg tgccccccct tctcgaaccc tcccggcccg ctaacgcggg cctcccatcc
      661 ccccaggggc tgcgcccctc ggccgcgaac ggcctcaccc caaaaatggc agccaagctg
      721 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
      781 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
      841 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
      901 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
      961 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     1021 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     1081 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     1141 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     1201 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     1261 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     1321 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     1381 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     1441 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     1501 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     1561 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     1621 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     1681 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     1741 ttttgctcac atgttcagcg cttgaacttg gcgtagcgct cgcgggcgat ttccagggtc
     1801 gagttgcgga cgcgctcgaa gcgctccttg tccttctgcc acagcgagcg gaccgccagg
     1861 ccgcggaccg agttgaagat cagccacagg atgtcctccg cgtcgtcgcg gctcaggccg
     1921 cgcgacacca gcacgcccag ccacatatcc tcgacgacga agcggttgcg ctcgacggtg
     1981 cgctggatgc cctcgcgcag cgccgggtcg cggtcggcgg ccacgatcag gtccagcgag
     2041 atcgagaagt cgtcgtccag gaagaactcg gcggcgtcgt ccagcatctg ctggatgacg
     2101 tcgtcctcgg gcttcagctt ggccaggcgg gcgcggctgc gctcggtgat ctgctcgtac
     2161 agccactcga aggtggccag cagcagttcc agcttggtcg ggaagtggtg cgactgggcg
     2221 ccgcggctga cgccggcggc gcccgggacg tcggcgatgc ggaagccggc atagcccttc
     2281 tcgcgcagga cgcccagggc ggcggcgatc agcttgccct gggtttccat cgcgcgctcg
     2341 gcctgggtgc ggcgcttcgg cgacatgatg accattcggg gtctctccct gctagcatca
     2401 gggttattgt atcatgagcg gatacatatg tcaatgtacc ggaacaaaca gacaatctgg
     2461 tctgtttgta catggttgac ggatcgccgc gaagccgcta actgcgccgg ttaacaaaca
     2521 gacaatctgg tctgtttgta actagtagag gaagcttccg catggtgagc aagggcgagg
     2581 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     2641 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagc
     2701 tgatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgggct
     2761 acggcgtgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     2821 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     2881 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     2941 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     3001 acagccacaa cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca
     3061 agatccgcca caacatcgag gacggcggcg tgcagctcgc cgaccactac cagcagaaca
     3121 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccagtcca
     3181 agctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     3241 ccgccgggat cactctcggc atggacgagc tgtacaagat taatatgact ctagagacaa
     3301 gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt
     3361 agattttgca taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat
     3421 ggcggccgca ttaggcaccc caggctttac actttatgct tccggctcgt ataatgtgtg
     3481 gattttgagt taggatccgg cgagattttc aggagctaag gaagctaaaa tggagaaaaa
     3541 aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc
     3601 atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt
     3661 tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc
     3721 ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat
     3781 atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc
     3841 gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt
     3901 ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt
     3961 cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga
     4021 caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct
     4081 gatgccgctg gcgattcagg ttcatcatgc cgtctgtgat ggcttccatg tcggcagaat
     4141 gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaaa cgcgtcgttc
     4201 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     4261 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     4321 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     4381 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     4441 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     4501 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     4561 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     4621 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     4681 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     4741 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     4801 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     4861 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     4921 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     4981 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     5041 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     5101 agtgagcgca acgcaattaa tacgcgtgga tccggcttac taaaagccag ataacagtat
     5161 gcgtatttgc gcgctgattt ttgcggtata agaatatata ctgatatgta tacccgaagt
     5221 atgtcaaaaa gaggtgtgct atgaagcagc gtattacagt gacagttgac agcgacagct
     5281 atcagttgct caaggcatat atgatgtcaa tatctccggt ctggtaagca caaccatgca
     5341 gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc
     5401 tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct gacgagaaca gggactggtg
     5461 aaatgcagtt taaggtttac acctataaaa gagagagccg ttatcgtctg tttgtggatg
     5521 tacagagtga tattattgac acgcccgggc gacggatggt gatccccctg gccagtgcac
     5581 gtctgctgtc agataaagtc tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa
     5641 gctggcgcat gatgaccacc gatatggcca gtgtgccggt ctccgttatc ggggaagaag
     5701 tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg
     5761 gaatataaat gtcaggctcc gttatacaca gccagtctgc aggtcgacca tagtgactgg
     5821 atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata
     5881 ttgatattta tatcatttta cgtttctcgt tcagctttct tgtacaaagt ggtgatatcg
     5941 ctagctagag gtaccgagct ccaattggga tccggactac aaggaccacg acggcgacta
     6001 taaggaccac gacatcgact acaaggatga cgacgacaag tgaggatccg gtgaagtgac
     6061 ccgcaaaaaa aaaggccagg ggatcgctcc cccggccttt tttttgaatt cactggccgt
     6121 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     6181 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     6241 acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct
     6301 gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata
     6361 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     6421 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     6481 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     6541 ggttaatgtc atgataataa tggtttctta gcaccctttc tcggtccttc aacgttcctg
     6601 acaacgagcc tccttttcgc caatccatcg acaatcaccg cgagtccctg ctcgaacgct
     6661 gcgtccggac cggcttcgtc gaaggcgtct atcgcggccc gcaacagcgg cgagagcgga
     6721 gcctgttcaa cggtgccgcc gcgctcgccg gcatcgctgt cgccggcctg ctcctcaagc
     6781 acggccccaa cagtgaagta gctgattgtc atcagcgcat tgacggcgtc cccggccgaa
     6841 aaacccgcct cgcagaggaa gcgaagctgc gcgtcggccg tttccatctg cggtgcgccc
     6901 ggtcgcgtgc cggcatggat gcgcgcgcca tcgcggtagg cgagcagcgc ctgcctgaag
     6961 ctgcgggcat tcccgatcag aaatgagcgc cagtcgtcgt cggctctcgg caccgaatgc
     7021 gtatgattct ccgccagcat ggcttcggcc agtgcgtcga gcagcgcccg cttgttcctg
     7081 aagtgccagt aaagcgccgg ctgctgaacc cccaaccgtt ccgccagttt gcgtgtcgtc
     7141 agaccgtcta cgccgacctc gttcaacagg tccagggcgg cacggatcac tgtattcggc
     7201 tgcaactttg tcatgattga cactttatca ctgataaaca taatatgtcc accaacttat
     7261 cagtgataaa gaatccgcgc gttcaatcgg accagcggag gctggtccgg aggccagacg
     7321 tgaaacccaa catacccctg atcgtaattc tgagcactgt cgcgctcgac gctgtcggca
     7381 tcggcctgat tatgccggtg ctgccgggcc tcctgcgcga tctggttcac tcgaacgacg
     7441 tcaccgccca ctatggcatt ctgctggcgc tgtatgcgtt ggtgcaattt gcctgcgcac
     7501 ctgtgctggg cgcgctgtcg gatcgtttcg ggcggcggcc aatcttgctc gtctcgctgg
     7561 ccggcgccac tgtcgactac gccatcatgg cgacagcgcc tttcctttgg gttctctata
     7621 tcgggcggat cgtggccggc atcaccgggg cgactggggc ggtagccggc gcttatattg
     7681 ccgatatcac tgatggcgat gagcgcgcgc ggcacttcgg cttcatgagc gcctgtttcg
     7741 ggttcgggat ggtcgcggga cctgtgctcg gtgggctgat gggcggtttc tccccccacg
     7801 ctccgttctt cgccgcggca gccttgaacg gcctcaattt cctgacgggc tgtttccttt
     7861 tgccggagtc gcacaaaggc gaacgccggc cgttacgccg ggaggctctc aacccgctcg
     7921 cttcgttccg gtgggcccgg ggcatgaccg tcgtcgccgc cctgatggcg gtcttcttca
     7981 tcatgcaact tgtcggacag gtgccggccg cgctttgggt cattttcggc gaggatcgct
     8041 ttcactggga cgcgaccacg atcggcattt cgcttgccgc atttggcatt ctgcattcac
     8101 tcgcccaggc aatgatcacc ggccctgtag ccgcccggct cggcgaaagg cgggcactca
     8161 tgctcggaat gattgccgac ggcacaggct acatcctgct tgccttcgcg acacggggat
     8221 ggatggcgtt cccgatcatg gtcctgcttg cttcgggtgg catcggaatg ccggcgctgc
     8281 aagcaatgtt gtccaggcag gtggatgagg aacgtcaggg gcagctgcaa ggctcactgg
     8341 cggcgctcac cagcctgacc tcgatcgtcg gacccctcct cttcacggcg atctatgcgg
     8401 cttctataac aacgtggaac gggtgggcat ggattgcagg cgctgccctc tacttgctct
     8461 gcctgccggc gctgcgtcgc gggctttgga gcggcgcagg gcaacgagcc gatcgctgat
     8521 cgtggaaacg ataggcctat gccatgcggg tcaaggcgac ttccggcaag ctatacgcgc
     8581 cctagaattg tcaattttaa tcctctgttt atcggcagtt cgtagagcgc gccgtgcgtc
     8641 ccgagcgata ctgagcgaag caagtgcgtc gagcagtgcc cgcttgttcc tgaaatgcca
     8701 gtaaagcgct ggctgctgaa cccccagccg gaactgaccc cacaaggccc tagcgtttgc
     8761 aatgcaccag gtcatcattg acccaggcgt gttccaccag gccgctgcct cgcaactctt
     8821 cgcaggcttc gccgacctgc tcgcgccact tcttcacgcg ggtggaatcc gatccgcaca
     8881 tgaggcggaa ggtttccagc ttgagcgggt acggctcccg gtgcgagctg aaatagtcga
     8941 acatccgtcg ggccgtcggc gacagcttgc ggtacttctc ccatatgaat ttcgtgtagt
     9001 ggtcgccagc aaacagcacg acgatttcct cgtcgatcag gacctggcaa cgggacgttt
     9061 tcttgccacg gtccaggacg cggaagcggt gcagcagcga caccgattcc aggtgcccaa
     9121 cgcggtcgga cgtgaagccc atcgccgtcg cctgtaggcg cgacaggcat tcctcggcct
     9181 tcgtgtaata ccggccattg atcgaccagc ccaggtcctg gcaaagctcg tagaacgtga
     9241 aggtgatcgg ctcgccgata ggggtgcgct tcgcgtactc caacacctgc tgccacacca
     9301 gttcgtcatc gtcggcccgc agctcgacgc cggtgtaggt gatcttcacg tccttgttga
     9361 cgtggaaaat gaccttgttt tgcagcgcct cgcgcgggat tttcttgttg cgcgtggtga
     9421 acagggcaga gcgggccgtg tcgtttggca tcgctcgcat cgtgtccggc cacggcgcaa
     9481 tatcgaacaa ggaaagctgc atttccttga tctgctgctt cgtgtgtttc agcaacgcgg
     9541 cctgcttggc ctcgctgacc tgttttgcca ggtcctcgcc ggcggttttt cgcttcttgg
     9601 tcgtcatagt tcctcgcgtg tcgatggtca tcgacttcgc caaacctgcc gcctcctgtt
     9661 cgagacgacg cgaacgctcc acggcggccg atggcgcggg cagggcaggg ggagccagtt
     9721 gcacgctgtc gcgctcgatc ttggccgtag cttgctggac catcgagccg acggactgga
     9781 aggtttcgcg gggcgcacgc atgacggtgc ggcttgcgat ggtttcggca tcctcggcgg
     9841 aaaaccccgc gtcgatcagt tcttgcctgt atgccttccg gtcaaacgtc cgattcattc
     9901 accctccttg cgggattgcc ccgactcacg ccggggcaat gtgcccttat tcctgatttg
     9961 acccgcctgg tgccttggtg tccagataat ccaccttatc ggcaatgaag tcggtcccgt
    10021 agaccgtctg gccgtccttc tcgtacttgg tattccgaat cttgccctgc acgaatacca
    10081 gctccgcgaa gtcgctcttc ttgatggagc gcatggggac gtgcttggca atcacgcgca
    10141 ccccccggcc gttttagcgg ctaaaaaagt catggctctg ccctcgggcg gaccacgccc
    10201 atcatgacct tgccaagctc gtcctgcttc tcttcgatct tcgccagcag ggcgaggatc
    10261 gtggcatcac cgaaccgcgc cgtgcgcggg tcgtcggtga gccagagttt cagcaggccg
    10321 cccaggcggc ccaggtcgcc attgatgcgg gccagctcgc ggacgtgctc atagtccacg
    10381 acgcccgtga ttttgtagcc ctggccgacg gccagcaggt aggcctacag gctcatgccg
    10441 gccgccgccg ccttttcctc aatcgctctt cgttcgtctg gaaggcagta caccttgata
    10501 ggtgggctgc ccttcctggt tggcttggtt tcatcagcca tccgcttgcc ctcatctgtt
    10561 acgccggcgg tagccggcca gcctcgcaga gcaggattcc cgttgagcac cgccaggtgc
    10621 gaataaggga cagtgaagaa ggaacacccg ctcgcgggtg ggcctacttc acctatcctg
    10681 cccggctgac gccgttggat acaccaagga aagtctacac gaaccctttg gcaaaatcct
    10741 gtatatcgtg cgaaaaagga tggatatacc gaaaaaatcg ctataatgac cccgaagcag
    10801 ggttatgcag cggaaaagat ccgtc
//
