LOCUS       sequence-69237-e        9903 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lacZ promoter-probe plasmid with pVS1 origin of replication and 
            chloramphenicol resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9903)
  AUTHORS   Kaczmarczyk A, Vorholt JA, Francez-Charlot A
  TITLE     Cumate-inducible gene expression system for sphingomonads and other 
            Alphaproteobacteria.
  JOURNAL   Appl Environ Microbiol. 2013 Nov;79(21):6795-802. doi: 
            10.1128/AEM.02296-13. Epub 2013 Aug 30.
  PUBMED    23995928
REFERENCE   2  (bases 1 to 9903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.02296-13"; journalName: "Appl Environ Microbiol"; date:
            "2013-11"; volume: "79"; issue: "21"; pages: "6795-802"
COMMENT     Sequence Label: pAK501
FEATURES             Location/Qualifiers
     source          1..9903
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     234..250
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     234..250
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             246..3320
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYG
                     QDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQ
                     ISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIID
                     ERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIEN
                     GLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLW
                     YTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWS
                     LGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVP
                     KWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSL
                     IKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQ
                     TIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQ
                     LWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELG
                     NKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWK
                     AAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDV
                     EVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPY
                     VFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDG
                     FHMGIGGDDSWSPSVSAELQLSAGRYHYQLVWCQK"
     primer_bind     complement(266..283)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(266..282)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(275..297)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        3938..4040
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             4041..4700
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(4107..4126)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     complement(4922..4939)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5056..5601)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(5136..5155)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      6311..6505
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(6571..7644)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(8073..8702)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gtctaagaac atggatggat atctgcagaa tccgcccttt tttgtttgcg ggggttcgcc
       61 ggtcacgcaa aaaaaggccg ggggagcgat cccccggcct ttttgtttgc gggggttcgc
      121 cggtcacgca aaaaaggcgg gggagcgatc ccccggcctt tttgtttgcg ggaagggcga
      181 atcccagcac actggcggca agctttttct agagggccgg atcccggtac ccacaggaaa
      241 cagctatgac catgattacg gattcactgg ccgtcgtttt acaacgtcgt gactgggaaa
      301 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
      361 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
      421 ggcgctttgc ctggtttccg gcaccagaag cggtgccgga aagctggctg gagtgcgatc
      481 ttcctgaggc cgatactgtc gtcgtcccct caaactggca gatgcacggt tacgatgcgc
      541 ccatctacac caacgtaacc tatcccatta cggtcaatcc gccgtttgtt cccacggaga
      601 atccgacggg ttgttactcg ctcacattta atgttgatga aagctggcta caggaaggcc
      661 agacgcgaat tatttttgat ggcgttaact cggcgtttca tctgtggtgc aacgggcgct
      721 gggtcggtta cggccaggac agtcgtttgc cgtctgaatt tgacctgagc gcatttttac
      781 gcgccggaga aaaccgcctc gcggtgatgg tgctgcgttg gagtgacggc agttatctgg
      841 aagatcagga tatgtggcgg atgagcggca ttttccgtga cgtctcgttg ctgcataaac
      901 cgactacaca aatcagcgat ttccatgttg ccactcgctt taatgatgat ttcagccgcg
      961 ctgtactgga ggctgaagtt cagatgtgcg gcgagttgcg tgactaccta cgggtaacag
     1021 tttctttatg gcagggtgaa acgcaggtcg ccagcggcac cgcgcctttc ggcggtgaaa
     1081 ttatcgatga gcgtggtggt tatgccgatc gcgtcacact acgtctgaac gtcgaaaacc
     1141 cgaaactgtg gagcgccgaa atcccgaatc tctatcgtgc ggtggttgaa ctgcacaccg
     1201 ccgacggcac gctgattgaa gcagaagcct gcgatgtcgg tttccgcgag gtgcggattg
     1261 aaaatggtct gctgctgctg aacggcaagc cgttgctgat tcgaggcgtt aaccgtcacg
     1321 agcatcatcc tctgcatggt caggtcatgg atgagcagac gatggtgcag gatatcctgc
     1381 tgatgaagca gaacaacttt aacgccgtgc gctgttcgca ttatccgaac catccgctgt
     1441 ggtacacgct gtgcgaccgc tacggcctgt atgtggtgga tgaagccaat attgaaaccc
     1501 acggcatggt gccaatgaat cgtctgaccg atgatccgcg ctggctaccg gcgatgagcg
     1561 aacgcgtaac gcgaatggtg cagcgcgatc gtaatcaccc gagtgtgatc atctggtcgc
     1621 tggggaatga atcaggccac ggcgctaatc acgacgcgct gtatcgctgg atcaaatctg
     1681 tcgatccttc ccgcccggtg cagtatgaag gcggcggagc cgacaccacg gccaccgata
     1741 ttatttgccc gatgtacgcg cgcgtggatg aagaccagcc cttcccggct gtgccgaaat
     1801 ggtccatcaa aaaatggctt tcgctacctg gagagacgcg cccgctgatc ctttgcgaat
     1861 acgcccacgc gatgggtaac agtcttggcg gtttcgctaa atactggcag gcgtttcgtc
     1921 agtatccccg tttacagggc ggcttcgtct gggactgggt ggatcagtcg ctgattaaat
     1981 atgatgaaaa cggcaacccg tggtcggctt acggcggtga ttttggcgat acgccgaacg
     2041 atcgccagtt ctgtatgaac ggtctggtct ttgccgaccg cacgccgcat ccagcgctga
     2101 cggaagcaaa acaccagcag cagtttttcc agttccgttt atccgggcaa accatcgaag
     2161 tgaccagcga atacctgttc cgtcatagcg ataacgagct cctgcactgg atggtggcgc
     2221 tggatggtaa gccgctggca agcggtgaag tgcctctgga tgtcgctcca caaggtaaac
     2281 agttgattga actgcctgaa ctaccgcagc cggagagcgc cgggcaactc tggctcacag
     2341 tacgcgtagt gcaaccgaac gcgaccgcat ggtcagaagc cgggcacatc agcgcctggc
     2401 agcagtggcg tctggcggaa aacctcagtg tgacgctccc cgccgcgtcc cacgccatcc
     2461 cgcatctgac caccagcgaa atggattttt gcatcgagct gggtaataag cgttggcaat
     2521 ttaaccgcca gtcaggcttt ctttcacaga tgtggattgg cgataaaaaa caactgctga
     2581 cgccgctgcg cgatcagttc acccgtgcac cgctggataa cgacattggc gtaagtgaag
     2641 cgacccgcat tgaccctaac gcctgggtcg aacgctggaa ggcggcgggc cattaccagg
     2701 ccgaagcagc gttgttgcag tgcacggcag atacacttgc tgatgcggtg ctgattacga
     2761 ccgctcacgc gtggcagcat caggggaaaa ccttatttat cagccggaaa acctaccgga
     2821 ttgatggtag tggtcaaatg gcgattaccg ttgatgttga agtggcgagc gatacaccgc
     2881 atccggcgcg gattggcctg aactgccagc tggcgcaggt agcagagcgg gtaaactggc
     2941 tcggattagg gccgcaagaa aactatcccg accgccttac tgccgcctgt tttgaccgct
     3001 gggatctgcc attgtcagac atgtataccc cgtacgtctt cccgagcgaa aacggtctgc
     3061 gctgcgggac gcgcgaattg aattatggcc cacaccagtg gcgcggcgac ttccagttca
     3121 acatcagccg ctacagtcaa cagcaactga tggaaaccag ccatcgccat ctgctgcacg
     3181 cggaagaagg cacatggctg aatatcgacg gtttccatat ggggattggt ggcgacgact
     3241 cctggagccc gtcagtatcg gcggaattac agctgagcgc cggtcgctac cattaccagt
     3301 tggtctggtg tcaaaaataa taataaccgg gcaggccatg tctgcccgtg ctagcttcac
     3361 gctgccgcaa gcactcaggg cgcaagggct gctaaaggaa gcggaacacg tagaaagcca
     3421 gtccgcagaa acggtgctga ccccggatga atgtcagcta ctgggctatc tggacaaggg
     3481 aaaacgcaag cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag
     3541 actgggcggt tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta
     3601 aggttgggaa gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc
     3661 gcaggggatc aagatccgct gatgtccggc ggtgcttttg ccgttacgca ccaccccgtc
     3721 agtagctgaa caggagggac agctgataga aacagaagcc actggagcac ctcaaaaaca
     3781 ccatcataca ctaaatcagt aagttggcag catcacccga cgcactttgc gccgaataaa
     3841 tacctgtgac ggaagatcac ttcgcagaat aaataaatcc tggtgtccct gttgataccg
     3901 ggaagccctg ggccaacttt tggcgaaaat gagacgttga tcggcacgta agaggttcca
     3961 actttcacca taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt
     4021 caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat
     4081 cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata
     4141 accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca
     4201 agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc
     4261 gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg
     4321 ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc
     4381 ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt
     4441 tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca
     4501 ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg
     4561 gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg
     4621 ccgtctgtga tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg
     4681 agtggcaggg cggggcgtaa tttttttaag gcagttattg gtgcccttaa acgcctggtg
     4741 ctaggcctgg tatgagtcag caacaccttc ttcacgaggc agacctcagc gctagcggag
     4801 tgtatactgg cttactatgt tggcactgat gagggtgtca gtgaagtgct tcatgtggca
     4861 ggagaaaaaa ggctgcaccg gtgcgtcagc agaatatgtg atacaggata tattccgctt
     4921 cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg
     4981 aacggggcgg agatttcctg gaagatgcca ggaagatact taacagggaa gtgagagggc
     5041 cgcggcaaag ccgtttttcc ataggctccg cccccctgac aagcatcacg aaatctgacg
     5101 ctcaaatcag tggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     5161 cggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca ttccgctgtt
     5221 atggccgcgt ttgtctcatt ccacgcctga cactcagttc cgggtaggca gttcgctcca
     5281 agctggactg tatgcacgaa ccccccgttc agtccgaccg ctgcgcctta tccggtaact
     5341 atcgtcttga gtccaacccg gaaagacatg caaaagcacc actggcagca gccactggta
     5401 attgatttag aggagttagt cttgaagtca tgcgccggtt aaggctaaac tgaaaggaca
     5461 agttttggtg actgcgctcc tccaagccag ttacctcggt tcaaagagtt ggtagctcag
     5521 agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt tttcagagca agagattacg
     5581 cgcagaccaa aacgatctca agaagatcat cttattaagg ggtctgacgc tcagtggaac
     5641 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     5701 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     5761 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     5821 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     5881 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     5941 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     6001 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     6061 cgcaacgttg ttgccattgc cctggtatga gtcagcaaca ccttcttcac gaggcagacc
     6121 tcagcgccgg cggtcgagtg gcgacggcgc ggcttgtccg cgccctggta gattgcctgg
     6181 ccgtaggcca gccatttttg agcggccagc ggccgcgata ggccgacgcg aagcggcggg
     6241 gcgtagggag cgcagcgacc gaagggtagg cgctttttgc agctcttcgg ctgtgcgctg
     6301 gccagacagt tatgcacagg ccaggcgggt tttaagagtt ttaataagtt ttaaagagtt
     6361 ttaggcggaa aaatcgcctt ttttctcttt tatatcagtc acttacatgt gtgaccggtt
     6421 cccaatgtac ggctttgggt tcccaatgta cgggttccgg ttcccaatgt acggctttgg
     6481 gttcccaatg tacgtgctat ccacaggaaa gagacctttt cgaccttttt cccctgctag
     6541 ggcaatttgc cctagcatct gctccgtaca ttaggaaccg gcggatgctt cgccctcgat
     6601 caggttgcgg tagcgcatga ctaggatcgg gccagcctgc cccgcctcct ccttcaaatc
     6661 gtactccggc aggtcatttg acccgatcag cttgcgcacg gtgaaacaga acttcttgaa
     6721 ctctccggcg ctgccactgc gttcgtagat cgtcttgaac aaccatctgg cttctgcctt
     6781 gcctgcggcg cggcgtgcca ggcggtagag aaaacggccg atgccgggat cgatcaaaaa
     6841 gtaatcgggg tgaaccgtca gcacgtccgg gttcttgcct tctgtgatct cgcggtacat
     6901 ccaatcagct agctcgatct cgatgtactc cggccgcccg gtttcgctct ttacgatctt
     6961 gtagcggcta atcaaggctt caccctcgga taccgtcacc aggcggccgt tcttggcctt
     7021 cttcgtacgc tgcatggcaa cgtgcgtggt gtttaaccga atgcaggttt ctaccaggtc
     7081 gtctttctgc tttccgccat cggctcgccg gcagaacttg agtacgtccg caacgtgtgg
     7141 acggaacacg cggccgggct tgtctccctt cccttcccgg tatcggttca tggattcggt
     7201 tagatgggaa accgccatca gtaccaggtc gtaatcccac acactggcca tgccggccgg
     7261 ccctgcggaa acctctacgt gcccgtctgg aagctcgtag cggatcacct cgccagctcg
     7321 tcggtcacgc ttcgacagac ggaaaacggc cacgtccatg atgctgcgac tatcgcgggt
     7381 gcccacgtca tagagcatcg gaacgaaaaa atctggttgc tcgtcgccct tgggcggctt
     7441 cctaatcgac ggcgcaccgg ctgccggcgg ttgccgggat tctttgcgga ttcgatcagc
     7501 ggccgcttgc cacgattcac cggggcgtgc ttctgcctcg atgcgttgcc gctgggcggc
     7561 ctgcgcggcc ttcaacttct ccaccaggtc atcacccagc gccgcgccga tttgtaccgg
     7621 gccggatggt ttgcgaccgc tcacgccgat tcctcgggct tgggggttcc agtgccattg
     7681 cagggccggc agacaaccca gccgcttacg cctggccaac cgcccgttcc tccacacatg
     7741 gggcattcca cggcgtcggt gcctggttgt tcttgatttt ccatgccgcc tcctttagcc
     7801 gctaaaattc atctactcat ttattcattt gctcatttac tctggtagct gcgcgatgta
     7861 ttcagatagc agctcggtaa tggtcttgcc ttggcgtacc gcgtacatct tcagcttggt
     7921 gtgatcctcc gccggcaact gaaagttgac ccgcttcatg gctggcgtgt ctgccaggct
     7981 ggccaacgtt gcagccttgc tgctgcgtgc gctcggacgg ccggcactta gcgtgtttgt
     8041 gcttttgctc attttctctt tacctcatta actcaaatga gttttgattt aatttcagcg
     8101 gccagcgcct ggacctcgcg ggcagcgtcg ccctcgggtt ctgattcaag aacggttgtg
     8161 ccggcggcgg cagtgcctgg gtagctcacg cgctgcgtga tacgggactc aagaatgggc
     8221 agctcgtacc cggccagcgc ctcggcaacc tcaccgccga tgcgcgtgcc tttgatcgcc
     8281 cgcgacacga caaaggccgc ttgtagcctt ccatccgtga cctcaatgcg ctgcttaacc
     8341 agctccacca ggtcggcggt ggcccatatg tcgtaagggc ttggctgcac cggaatcagc
     8401 acgaagtcgg ctgccttgat cgcggacaca gccaagtccg ccgcctgggg cgctccgtcg
     8461 atcactacga agtcgcgccg gccgatggcc ttcacgtcgc ggtcaatcgt cgggcggtcg
     8521 atgccgacaa cggttagcgg ttgatcttcc cgcacggccg cccaatcgcg ggcactgccc
     8581 tggggatcgg aatcgactaa cagaacatcg gccccggcga gttgcagggc gcgggctaga
     8641 tgggttgcga tggtcgtctt gcctgacccg cctttctggt taagtacagc gataaccttc
     8701 atgcgttccc cttgcgtatt tgtttattta ctcatcgcat catatacgca gcgaccgcat
     8761 gacgcaagct gttttactca aatacacatc acctttttag acggcggcgc tcggtttctt
     8821 cagcggccaa gctggccggc caggccgcca gcttggcatc agacaaaccg gccaggattt
     8881 catgcagccg cacggttgag acgtgcgcgg gcggctcgaa cacgtacccg gccgcgatca
     8941 tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc ctggccgtcc tggtgcggtt
     9001 tcatgcttgt tcctcttggc gttcattctc ggcggccgcc agggcgtcgg cctcggtcaa
     9061 tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg ggcgtcactt cctcgctgcg
     9121 ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc agtgcagccg cctctttcac
     9181 ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg atctgtgccg gggtgagggt
     9241 agggcggggg ccaaacttca cgcctcgggc cttggcggcc tcgcgcccgc tccgggtgcg
     9301 gtcgatgatt agggaacgct cgaactcggc aatgccggcg aacacggtca acaccatgcg
     9361 gccggccggc gtggtggtgt cggcccacgg ctctgccagg ctacgcaggc ccgcgccggc
     9421 ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg ctgcgggcca ggcggtctag
     9481 cctggtcact gtcacaacgt cgccagggcg taggtggtca agcatcctgg ccagctccgg
     9541 gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc ttggtgcagc cggccgcgtg
     9601 cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg ctgacgcggg catagcccag
     9661 caggccagcg gcggcgctct tgttcatggc gtaatgtctc cggttctagt cgcaagtatt
     9721 ctactttatg cgactaaaac acgcgacaag aaaacgccag gaaaagggca gggcggcagc
     9781 ctgtcgcgta acttaggact tgtgcgacat gtcgttttca gaagacggct gcactgaacg
     9841 tcagaagccg actgcactat agcagcggag gggttggatc ttccccgaaa agtgccacct
     9901 gac
//
