LOCUS       sequence-69238-e         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses E.coli codon optimized EYFP-Twin-Strep fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Dammeyer T, Timmis KN, Tinnefeld P
  TITLE     Broad host range vectors for expression of proteins with (Twin-) 
            Strep-tag, His-tag and engineered, export optimized yellow 
            fluorescent protein.
  JOURNAL   Microb Cell Fact. 2013 May 20;12(1):49.
  PUBMED    23687945
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2013-05-20"; volume: "12(1)"; pages: "49"
COMMENT     Sequence Label: pTD-C_eYFPTwinStrep
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             760..783
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             820..843
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 atggatccga attcgatcgt ttctaaaggt gaagaactgt tcaccggtgt tgttccgatc
       61 ctggttgaac tggacggtga cgttaacggt cacaaattct ctgtttctgg tgaaggtgaa
      121 ggtgacgcta cctacggtaa actgaccctg aaattcatct gcaccaccgg taaactgccg
      181 gttccgtggc cgaccctggt taccaccttc ggttacggtc tgcagtgctt cgctcgttac
      241 ccggaccaca tgaaacagca cgacttcttc aaatctgcta tgccggaagg ttacgttcag
      301 gaacgtacca tcttcttcaa agacgacggt aactacaaaa cccgtgctga agttaaattc
      361 gaaggtgaca ccctggttaa ccgtatcgaa ctgaaaggta tcgacttcaa agaagacggt
      421 aacatcctgg gtcacaaact ggaatacaac tacaactctc acaacgttta catcatggct
      481 gacaaacaga aaaacggtat caaagttaac ttcaaaatcc gtcacaacat cgaagacggt
      541 tctgttcagc tggctgacca ctaccagcag aacaccccga tcggtgacgg tccggttctg
      601 ctgccggaca accactacct gtcttaccag tctaaactgt ctaaagaccc gaacgaaaaa
      661 cgtgaccaca tggttctgct ggaatttgtt accgctgctg gtatcaccct gggtatggac
      721 gaactgtaca aatctagagc ggccgcactc gagagcgctt ggagccaccc gcagttcgag
      781 aaaggtggag gttccggagg tggatcggga ggtggatcgt ggagccaccc gcagttcgaa
      841 aaa
//
