LOCUS       sequence-69241-e         186 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  use to create N-terminal Strep-tagII fusion proteins or dual tagged 
            fusion with N-terminal Strep-tagII and C-terminal 6x His-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 186)
  AUTHORS   Dammeyer T, Timmis KN, Tinnefeld P
  TITLE     Broad host range vectors for expression of proteins with (Twin-) 
            Strep-tag, His-tag and engineered, export optimized yellow 
            fluorescent protein.
  JOURNAL   Microb Cell Fact. 2013 May 20;12(1):49.
  PUBMED    23687945
REFERENCE   2  (bases 1 to 186)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2013-05-20"; volume: "12(1)"; pages: "49"
COMMENT     Sequence Label: pTD-NStrepHis
FEATURES             Location/Qualifiers
     source          1..186
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..57
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             154..171
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 caattgttta aaggagatat acaaatggct agctggagcc acccgcagtt cgaaaaaggc
       61 gccgagaccg cggtcccgaa ttcgagctcg gtacccgggg atccctcgag gtcgacctgc
      121 agggggacca tggtctcagg cctgagagga tcgcatcacc atcaccatca ctaatttaaa
      181 aagctt
//
