LOCUS       sequence-69244-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses P.putida codon optimized PelB-sfYFP-Twin-Strep fusion 
            protein for periplasmic translocation via PelB signal sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Dammeyer T, Timmis KN, Tinnefeld P
  TITLE     Broad host range vectors for expression of proteins with (Twin-) 
            Strep-tag, His-tag and engineered, export optimized yellow 
            fluorescent protein.
  JOURNAL   Microb Cell Fact. 2013 May 20;12(1):49.
  PUBMED    23687945
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2013-05-20"; volume: "12(1)"; pages: "49"
COMMENT     Sequence Label: pTDpelB-C_sfYFPTwinStrep
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             757..780
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             817..840
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 atggatccga attcggtgtc gaagggcgaa gaactgttca ccggcgtggt gccgatcctg
       61 gtggaactgg acggcgacgt gaacggccac aagttccgcg tgtcgggcga aggcgaaggc
      121 gacgccacca acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgccggtg
      181 ccgtggccga ccctggtgac caccctgggc tacggcctgc agtgcttcgc ccgctacccg
      241 gaccacatga agcagcacga cttcttcaag tcggccatgc cggaaggcta cgtgcaggaa
      301 cgcaccatct cgttcaagga cgacggcacc tacaagaccc gcgccgaagt gaagttcgaa
      361 ggcgacaccc tggtgaaccg catcgaactg aagggcatcg acttcaagga agacggcaac
      421 atcctgggcc acaagctgga atacaacttc aactcgcaca acgtgtacat caccgccgac
      481 aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacgtgga agacggcggc
      541 gtgcagctgg ccgaccacta ccagcagaac accccgatcg gcgacggccc ggtgctgctg
      601 ccggacaacc actacctgtc gtaccagtcg aagctgtcga aggacccgaa cgaaaagcgc
      661 gaccacatgg tgctgctgga atttgtgacc gccgccggca tcaccctggg catggacgaa
      721 ctgtacaagt ctagagcggc cgcactcgag agcgcttgga gccacccgca gttcgagaaa
      781 ggtggaggtt ccggaggtgg atcgggaggt ggatcgtgga gccacccgca gttcgaaaaa
//
