LOCUS       sequence-69245-e         309 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  use to create N-terminal PelB-Twin-Strep-tag fusion proteins or dual
            tagged fusion with N-terminal PelB-Twin-Strep-tag and C-terminal 6x 
            His-tag for periplasmic translocation via PelB signal sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Dammeyer T, Timmis KN, Tinnefeld P
  TITLE     Broad host range vectors for expression of proteins with (Twin-) 
            Strep-tag, His-tag and engineered, export optimized yellow 
            fluorescent protein.
  JOURNAL   Microb Cell Fact. 2013 May 20;12(1):49.
  PUBMED    23687945
REFERENCE   2  (bases 1 to 309)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2013-05-20"; volume: "12(1)"; pages: "49"
COMMENT     Sequence Label: pTDpelB-NTwinStrep
FEATURES             Location/Qualifiers
     source          1..309
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     28..93
                     /codon_start=1
                     /gene="pelB (fragment)"
                     /product="leader peptide for secretion"
                     /label=pelB signal sequence
                     /translation="MKYLLPTAAAGLLLLAAQPAMA"
     CDS             join(94..99,100..123,124..159,160..183)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             280..297
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cgtagccaat tggaaggaga tatacaaatg aagtacctgc tgccgaccgc cgccgccggc
       61 ctgctgctgc tggccgccca gccggccatg gccagcgctt ggagccaccc gcagttcgag
      121 aaaggtggag gttccggagg tggatcggga ggttcggcgt ggagccaccc gcagttcgaa
      181 aaaggcgccg agaccgcggt cccgaattcg agctcggtac ccggggatcc ctcgaggtcg
      241 acctgcaggg ggaccatggt ctcaggcctg agaggatcgc atcaccatca ccatcactaa
      301 aagcttgtc
//
