LOCUS       sequence-69250-e         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PcHA-Bmal backbone used for the above mutation. Ref.Genes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Huang N, Chelliah Y, Shan Y, Taylor CA, Yoo SH, Partch C, Green CB, 
            Zhang H, Takahashi JS
  TITLE     Crystal structure of the heterodimeric CLOCK:BMAL1 transcriptional 
            activator complex.
  JOURNAL   Science. 2012 Jul 13;337(6091):189-94. doi: 10.1126/science.1222804.
            Epub 2012 May 31.
  PUBMED    22653727
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1222804"; journalName: "Science"; date: 
            "2012-07-13- 13"; volume: "337"; issue: "6091"; pages: "189-94"
COMMENT     Sequence Label: pCDNA3-HA/Bmal L115E
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             82..108
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttcga tcgtcgacat gtacccatac gatgttccag attacgcttc tagaggatcc
      121 gcggaccaga gaatggacat ttcctcaacc atcagcgact tcatgtctcc gggccccacc
      181 gacctactct ccggttccct gggcaccagt ggtgtggact gcaatcgcaa gaggaaaggc
      241 agtgccactg actaccaaga aagtatggac acagacaaag atgaccctca tggaaggtta
      301 gaatatgcag aacaccaagg aaggatcaag aatgcaaggg aggcccacag tcagattgaa
      361 aagaggcgtc gggacaaaat gaacagtttc attgatgaat tggcttcttt ggtaccaaca
      421 tgcaatgcaa tgtccaggaa gttagataaa ctcaccgtgg agaggatggc tgttcagcac
      481 atgaaaactt tgagaggtgc caccaaccca tacacagaag caaactacaa gccaacattt
      541 ctatcagatg acgaactgaa acacctaatt ctcagggcag cagatggatt tttgtttgtc
      601 gtaggatgtg accgagggaa gatcctcttt gtctccgagt ctgtcttcaa gatcctcaat
      661 tatagccaga atgaccttat tggccagagc ttgtttgact acctgcatcc aaaagatatt
      721 gccaaagtta aggaacagct atcttcctcg gacactgcgc cccgggagcg actcattgat
      781 gccaagactg gacttccggt taaaacggat ataacccctg ggccctcccg gctatgctct
      841 ggagcccgc
//
