LOCUS       sequence-69252-e         256 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  use to create N-terminal PelB and C-terminal Twin-Strep-tagged 
            fusion proteins for periplasmic translocation via PelB signal 
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Dammeyer T, Timmis KN, Tinnefeld P
  TITLE     Broad host range vectors for expression of proteins with (Twin-) 
            Strep-tag, His-tag and engineered, export optimized yellow 
            fluorescent protein.
  JOURNAL   Microb Cell Fact. 2013 May 20;12(1):49.
  PUBMED    23687945
REFERENCE   2  (bases 1 to 256)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2013-05-20"; volume: "12(1)"; pages: "49"
COMMENT     Sequence Label: pTDpelB-CTwinStrep
FEATURES             Location/Qualifiers
     source          1..256
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     38..103
                     /codon_start=1
                     /gene="pelB (fragment)"
                     /product="leader peptide for secretion"
                     /label=pelB signal sequence
                     /translation="MKYLLPTAAAGLLLLAAQPAMA"
     CDS             join(149..154,155..178,179..214,215..238)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK"
ORIGIN
        1 cgtagccaat tgttaacttt aagaaggaga tatacatatg aagtacctgc tgccgaccgc
       61 cgccgccggc ctgctgctgc tggccgccca gccggccatg gccgatccga attcgagctc
      121 cgtcgactct agagcggccg cactcgagag cgcttggagc cacccgcagt tcgagaaagg
      181 tggaggttcc ggaggtggat cgggaggttc ggcgtggagc cacccgcagt tcgaaaaata
      241 atgatgaaag cttgtc
//
