LOCUS       sequence-69254-e         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PcHA-Bmal backbone used for the above mutation. Ref.Genes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Huang N, Chelliah Y, Shan Y, Taylor CA, Yoo SH, Partch C, Green CB, 
            Zhang H, Takahashi JS
  TITLE     Crystal structure of the heterodimeric CLOCK:BMAL1 transcriptional 
            activator complex.
  JOURNAL   Science. 2012 Jul 13;337(6091):189-94. doi: 10.1126/science.1222804.
            Epub 2012 May 31.
  PUBMED    22653727
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1222804"; journalName: "Science"; date: 
            "2012-07-13- 13"; volume: "337"; issue: "6091"; pages: "189-94"
COMMENT     Sequence Label: pCDNA3-HA/Bmal I317D
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..51
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        32..50
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             78..104
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag
       61 cttcgatcgt cgacatgtac ccatacgatg ttccagatta cgcttctaga ggatccgcgg
      121 accagagaat ggacatttcc tcaaccatca gcgacttcat gtctccgggc cccaccgacc
      181 tactctccgg ttccctgggc accagtggtg tggactgcaa tcgcaagagg aaaggcagtg
      241 ccactgacta ccaagaaagt atggacacag acaaagatga ccctcatgga aggttagaat
      301 atgcagaaca ccaaggaagg atcaagaatg caagggaggc ccacagtcag attgaaaaga
      361 ggcgtcggga caaaatgaac agtttcattg atgaattggc ttctttggta ccaacatgca
      421 atgcaatgtc caggaagtta gataaactca ccgtgctaag gatggctgtt cagcacatga
      481 aaactttgag aggtgccacc aacccataca cagaagcaaa ctacaagcca acatttctat
      541 cagatgacga actgaaacac ctaattctca gggcagcaga tggatttttg tttgtcgtag
      601 gatgtgaccg agggaagatc ctctttgtct ccgagtctgt cttcaagatc ctcaattata
      661 gccagaatga ccttattggc cagagcttgt ttgactacct gcatccaaaa gatattgcca
      721 aagttaagga acagctatct tcctcggaca ctgcgccccg ggagcgactc attgatgcca
      781 agactggact tccggttaaa acggatataa cccctgggcc ctcccggcta tgctctggag
      841 cccgccgctc tttcttctgt agaatgaagt gcaacagg
//
